Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice

Cheng chin Hsu, Hui ting Yang, Jing jing Ho, Mei chin Yin, Jen ying Hsu

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9 Citations (Scopus)

Abstract

Purpose: The anti-glycative and anti-oxidative effects from Houttuynia cordata leaves aqueous extract (HCAE) in heart and kidney of diabetic mice were examined. Methods: HCAE, at 1 or 2 %, was supplied in drinking water for 8 weeks. Plasma glucose and blood urea nitrogen (BUN) levels and creatine phosphokinase (CPK) activity were measured. The production of oxidative and inflammatory factors was determined. Activity and protein expression of associated enzymes or regulators were analyzed. Results: HCAE intake at both doses lowered plasma glucose and BUN levels, and CPK activity and also restored creatinine clearance rate in diabetic mice. HCAE intake, only at 2 %, retained plasma insulin levels (P < 0.05). HCAE reduced reactive oxygen species, protein carbonyl, interleukin-6, tumor necrosis factor-alpha, Nε-(carboxymethyl)-lysine, pentosidine and fructose levels, and reserved glutathione content in heart and kidney of diabetic mice (P < 0.05). Diabetes enhanced aldose reductase (AR) activity and protein expression in heart and kidney (P < 0.05). HCAE intake at both doses decreased renal AR activity and protein expression, but only at 2 % lowered cardiac AR activity and protein expression (P < 0.05). Diabetes increased protein expression of RAGE, p47phox and gp91phox, nuclear factor kappa-B (NF-κB) p50, NF-κB p65 and mitogen-activated protein kinase in heart and kidney (P < 0.05). HCAE intake only at 2 % limited RAGE expression, but at 1 and 2 % downregulated p47phox, NF-κB p65 and p-p38 expression in these organs (P < 0.05). Conclusions: These findings suggest that Houttuynia cordata leaves aqueous extract could ameliorate cardiac and renal injury under diabetic condition.

Original languageEnglish
Pages (from-to)845-854
Number of pages10
JournalEuropean Journal of Nutrition
Volume55
Issue number2
DOIs
Publication statusPublished - Mar 1 2016
Externally publishedYes

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Keywords

  • Diabetes
  • Glycation
  • Houttuynia cordata
  • NADPH oxidase
  • Polyol pathway

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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