HOTAIR is a REST-regulated lncRNA that promotes neuroendocrine differentiation in castration resistant prostate cancer

Yi Ting Chang, Tzu Ping Lin, Jui Ting Tang, Mel Campbell, Yun Li Luo, Shih Yen Lu, Chia Pei Yang, Ting Yu Cheng, Ching Hsin Chang, Tze Tze Liu, Chi Hung Lin, Hsing Jein Kung, Chin Chen Pan, Pei Ching Chang

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21 Citations (Scopus)

Abstract

Long non-coding RNAs (lncRNAs) are emerging as novel diagnostic markers of prostate cancer (PCa) and new determinants of castration-resistant PCa (CRPC), an aggressive and metastatic form of PCa. In addition to androgen receptor (AR) signaling, neuroendocrine differentiation (NED) is associated with CRPC. Recent reports demonstrate that the downregulation of repressor element-1 silencing transcription factor (REST) protein is a key step in NED of PCa cells. Here, we report HOTAIR as a novel REST-repressed lncRNA that is upregulated in NED PCa cells and in CRPC. HOTAIR overexpression is sufficient to induce, whereas knockdown of HOTAIR suppressed NED of PCa cells. Gene ontology (GO) analysis of differentially expressed genes under HOTAIR overexpression and in CRPC versus benign prostatic hyperplasia (BPH) suggests that HOTAIR may participate in PCa progression. Taken together, our results provide the first evidence of lncRNA HOTAIR as a driver for NED of PCa cells.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalCancer Letters
Volume433
DOIs
Publication statusPublished - Oct 1 2018

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Keywords

  • Castration-resistant prostate cancer
  • HOTAIR
  • Long non-coding RNAs
  • Neuroendocrine differentiation
  • REST

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Chang, Y. T., Lin, T. P., Tang, J. T., Campbell, M., Luo, Y. L., Lu, S. Y., Yang, C. P., Cheng, T. Y., Chang, C. H., Liu, T. T., Lin, C. H., Kung, H. J., Pan, C. C., & Chang, P. C. (2018). HOTAIR is a REST-regulated lncRNA that promotes neuroendocrine differentiation in castration resistant prostate cancer. Cancer Letters, 433, 43-52. https://doi.org/10.1016/j.canlet.2018.06.029