Abstract

Aim: To investigate the effect of hot water-extracted Lycium barbarum (LBE) and Rehmannia glutinosa (RGE) on cell proliferation and apoptosis in rat and/or human hepatocellular carcinoma (HCC) cells. Methods: Rat (H-4-II-E) and human HCC (HA22T/VGH) cell lines were incubated with various concentrations (0-10 g/L) of hot water-extracted LBE and RGE. After 6-24 h incubation, cell proliferation (n = 6) was measured by a colorimetric method. The apoptotic cells (n = 6) were detected by flow cytometry. The expression of p53 protein (n = 3) was determined by SDS-PAGE and Western blotting. Results: Crude LBE (2-5 g/L) and RGE (2-10 g/L) dose-dependently inhibited proliferation of H-4-II-E cells by 11% (P <0.05) to 85% (P <0.01) after 6-24 h treatment. Crude LBE at a dose of 5 g/L suppressed cell proliferation of H-4-II-E cells more effectively than crude RGE after 6-24 h incubation (P <0.01). Crude LBE (2-10 g/L) and RGE (2-5 g/ L) also dose-dependently inhibited proliferation of HA22T/VGH cells by 14%-43% (P <0.01) after 24 h. Crude LBE at a dose of 10 g/L inhibited the proliferation of HA22T/VGH cells more effectively than crude RGE (56.8% ± 1.6% vs 70.3% ± 3.1% of control, P = 0.0003 <0.01). The apoptotic cells significantly increased in H-4-II-E cells after 24 h treatment with higher doses of crude LBE (2-5 g/L) and RGE (5-10 g/L) (P <0.01). The expression of p53 protein in H-4-II-E cells was 119% and 143% of the control group compared with the LBE-treated (2, 5 g/L) groups, and 110% and 132% of the control group compared with the RGE -treated (5, 10 g/L) groups after 24 h. Conclusion: Hot water-extracted crude LBE (2-5 g/L) and RGE (5-10 g/L) inhibit proliferation and stimulate p53-mediated apoptosis in HCC cells.

Original languageEnglish
Pages (from-to)4478-4484
Number of pages7
JournalWorld Journal of Gastroenterology
Volume12
Issue number28
Publication statusPublished - Jul 28 2006

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Lycium
Rehmannia
Hepatocellular Carcinoma
Apoptosis
Water
Cell Proliferation
Control Groups
Polyacrylamide Gel Electrophoresis
Flow Cytometry
Proteins

Keywords

  • Apoptosis
  • Hepatocellular carcinoma
  • Lycium barbarum extract
  • Proliferation
  • Rehmannia glutinosa extract

ASJC Scopus subject areas

  • Gastroenterology

Cite this

@article{049c9fa34833416e901487c61af592a8,
title = "Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells",
abstract = "Aim: To investigate the effect of hot water-extracted Lycium barbarum (LBE) and Rehmannia glutinosa (RGE) on cell proliferation and apoptosis in rat and/or human hepatocellular carcinoma (HCC) cells. Methods: Rat (H-4-II-E) and human HCC (HA22T/VGH) cell lines were incubated with various concentrations (0-10 g/L) of hot water-extracted LBE and RGE. After 6-24 h incubation, cell proliferation (n = 6) was measured by a colorimetric method. The apoptotic cells (n = 6) were detected by flow cytometry. The expression of p53 protein (n = 3) was determined by SDS-PAGE and Western blotting. Results: Crude LBE (2-5 g/L) and RGE (2-10 g/L) dose-dependently inhibited proliferation of H-4-II-E cells by 11{\%} (P <0.05) to 85{\%} (P <0.01) after 6-24 h treatment. Crude LBE at a dose of 5 g/L suppressed cell proliferation of H-4-II-E cells more effectively than crude RGE after 6-24 h incubation (P <0.01). Crude LBE (2-10 g/L) and RGE (2-5 g/ L) also dose-dependently inhibited proliferation of HA22T/VGH cells by 14{\%}-43{\%} (P <0.01) after 24 h. Crude LBE at a dose of 10 g/L inhibited the proliferation of HA22T/VGH cells more effectively than crude RGE (56.8{\%} ± 1.6{\%} vs 70.3{\%} ± 3.1{\%} of control, P = 0.0003 <0.01). The apoptotic cells significantly increased in H-4-II-E cells after 24 h treatment with higher doses of crude LBE (2-5 g/L) and RGE (5-10 g/L) (P <0.01). The expression of p53 protein in H-4-II-E cells was 119{\%} and 143{\%} of the control group compared with the LBE-treated (2, 5 g/L) groups, and 110{\%} and 132{\%} of the control group compared with the RGE -treated (5, 10 g/L) groups after 24 h. Conclusion: Hot water-extracted crude LBE (2-5 g/L) and RGE (5-10 g/L) inhibit proliferation and stimulate p53-mediated apoptosis in HCC cells.",
keywords = "Apoptosis, Hepatocellular carcinoma, Lycium barbarum extract, Proliferation, Rehmannia glutinosa extract, Apoptosis, Hepatocellular carcinoma, Lycium barbarum extract, Proliferation, Rehmannia glutinosa extract",
author = "Chao, {Jane C J} and Chiang, {Shih Wen} and Wang, {Ching Chiung} and Ya-Hui Tsai and Wu, {Ming Shun}",
year = "2006",
month = "7",
day = "28",
language = "English",
volume = "12",
pages = "4478--4484",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "28",

}

TY - JOUR

T1 - Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells

AU - Chao, Jane C J

AU - Chiang, Shih Wen

AU - Wang, Ching Chiung

AU - Tsai, Ya-Hui

AU - Wu, Ming Shun

PY - 2006/7/28

Y1 - 2006/7/28

N2 - Aim: To investigate the effect of hot water-extracted Lycium barbarum (LBE) and Rehmannia glutinosa (RGE) on cell proliferation and apoptosis in rat and/or human hepatocellular carcinoma (HCC) cells. Methods: Rat (H-4-II-E) and human HCC (HA22T/VGH) cell lines were incubated with various concentrations (0-10 g/L) of hot water-extracted LBE and RGE. After 6-24 h incubation, cell proliferation (n = 6) was measured by a colorimetric method. The apoptotic cells (n = 6) were detected by flow cytometry. The expression of p53 protein (n = 3) was determined by SDS-PAGE and Western blotting. Results: Crude LBE (2-5 g/L) and RGE (2-10 g/L) dose-dependently inhibited proliferation of H-4-II-E cells by 11% (P <0.05) to 85% (P <0.01) after 6-24 h treatment. Crude LBE at a dose of 5 g/L suppressed cell proliferation of H-4-II-E cells more effectively than crude RGE after 6-24 h incubation (P <0.01). Crude LBE (2-10 g/L) and RGE (2-5 g/ L) also dose-dependently inhibited proliferation of HA22T/VGH cells by 14%-43% (P <0.01) after 24 h. Crude LBE at a dose of 10 g/L inhibited the proliferation of HA22T/VGH cells more effectively than crude RGE (56.8% ± 1.6% vs 70.3% ± 3.1% of control, P = 0.0003 <0.01). The apoptotic cells significantly increased in H-4-II-E cells after 24 h treatment with higher doses of crude LBE (2-5 g/L) and RGE (5-10 g/L) (P <0.01). The expression of p53 protein in H-4-II-E cells was 119% and 143% of the control group compared with the LBE-treated (2, 5 g/L) groups, and 110% and 132% of the control group compared with the RGE -treated (5, 10 g/L) groups after 24 h. Conclusion: Hot water-extracted crude LBE (2-5 g/L) and RGE (5-10 g/L) inhibit proliferation and stimulate p53-mediated apoptosis in HCC cells.

AB - Aim: To investigate the effect of hot water-extracted Lycium barbarum (LBE) and Rehmannia glutinosa (RGE) on cell proliferation and apoptosis in rat and/or human hepatocellular carcinoma (HCC) cells. Methods: Rat (H-4-II-E) and human HCC (HA22T/VGH) cell lines were incubated with various concentrations (0-10 g/L) of hot water-extracted LBE and RGE. After 6-24 h incubation, cell proliferation (n = 6) was measured by a colorimetric method. The apoptotic cells (n = 6) were detected by flow cytometry. The expression of p53 protein (n = 3) was determined by SDS-PAGE and Western blotting. Results: Crude LBE (2-5 g/L) and RGE (2-10 g/L) dose-dependently inhibited proliferation of H-4-II-E cells by 11% (P <0.05) to 85% (P <0.01) after 6-24 h treatment. Crude LBE at a dose of 5 g/L suppressed cell proliferation of H-4-II-E cells more effectively than crude RGE after 6-24 h incubation (P <0.01). Crude LBE (2-10 g/L) and RGE (2-5 g/ L) also dose-dependently inhibited proliferation of HA22T/VGH cells by 14%-43% (P <0.01) after 24 h. Crude LBE at a dose of 10 g/L inhibited the proliferation of HA22T/VGH cells more effectively than crude RGE (56.8% ± 1.6% vs 70.3% ± 3.1% of control, P = 0.0003 <0.01). The apoptotic cells significantly increased in H-4-II-E cells after 24 h treatment with higher doses of crude LBE (2-5 g/L) and RGE (5-10 g/L) (P <0.01). The expression of p53 protein in H-4-II-E cells was 119% and 143% of the control group compared with the LBE-treated (2, 5 g/L) groups, and 110% and 132% of the control group compared with the RGE -treated (5, 10 g/L) groups after 24 h. Conclusion: Hot water-extracted crude LBE (2-5 g/L) and RGE (5-10 g/L) inhibit proliferation and stimulate p53-mediated apoptosis in HCC cells.

KW - Apoptosis

KW - Hepatocellular carcinoma

KW - Lycium barbarum extract

KW - Proliferation

KW - Rehmannia glutinosa extract

KW - Apoptosis

KW - Hepatocellular carcinoma

KW - Lycium barbarum extract

KW - Proliferation

KW - Rehmannia glutinosa extract

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M3 - Article

C2 - 16874858

AN - SCOPUS:33747516825

VL - 12

SP - 4478

EP - 4484

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 28

ER -