Hormonal regulation of the gene for the type C ecotropic retrovirus receptor in rat liver cells

Jin Yun Wu, Dan Robinson, Hsing Jien Kung, Maria Hatzoglou

Research output: Contribution to journalArticle

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Abstract

The infectibility of the regenerating rat liver by ecotropic retroviruses was studied relative to the expression of the gene coding for the ecotropic retrovirus receptor (Ecor) that functions as a cationic amino acid transporter. It is known that the gene for the receptor is expressed in primary hepatocytes and hepatoma cells but is absent in adult liver cells. Isolation of a 2.85-kb cDNA for the rat Ecor suggested that the rat viral receptor is 97% homologous to the mouse viral receptor and that it contains the envelope-binding domain that determines the host range of ecotropic murine retroviruses. This explains the efficient infection of rat cells by ecotropic retroviruses. Since cell division is required for liver cells to be infected, we determined the susceptibility of the regenerating rat liver to infection at different time points after partial hepatectomy (0 to 24 h) in relation to the presence of receptor mRNA. Infection of the liver occurred only when the liver was exposed to virus 4 h after partial hepatectomy. This time course of infection paralleled expression of the gene for the Ecor, which was rapidly induced between 2 and 6 h during liver regeneration. However, expression of the dormant receptor gene in quiescent liver cells can be induced by insulin, dexamethasone, and arginine, indicating that cell division is not required for expression of the receptor gene in liver cells. A diet high in carbohydrate (low in protein) significantly increased the concentration of receptor mRNA in liver cells, indicating that hormones play a role in the regulation of expression of this gene in vivo. We conclude that the gene for the viral receptor is expressed in the regenerating and quiescent liver when the urea cycle enzymes are down regulated. The infection of the regenerating rat liver by ecotropic retroviruses at the time point of expression of the receptor gene supports the requirement of expression of this transporter for infection.

Original languageEnglish
Pages (from-to)1615-1623
Number of pages9
JournalJournal of Virology
Volume68
Issue number3
Publication statusPublished - Mar 1 1994
Externally publishedYes

Fingerprint

Retroviridae
hormonal regulation
hepatocytes
receptors
Liver
rats
Genes
genes
liver
Infection
Gene Expression
infection
Hepatectomy
Cell Division
Basic Amino Acid Transport Systems
cell division
urea cycle enzymes
liver regeneration
amino acid transporters
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Hormonal regulation of the gene for the type C ecotropic retrovirus receptor in rat liver cells. / Wu, Jin Yun; Robinson, Dan; Kung, Hsing Jien; Hatzoglou, Maria.

In: Journal of Virology, Vol. 68, No. 3, 01.03.1994, p. 1615-1623.

Research output: Contribution to journalArticle

Wu, Jin Yun ; Robinson, Dan ; Kung, Hsing Jien ; Hatzoglou, Maria. / Hormonal regulation of the gene for the type C ecotropic retrovirus receptor in rat liver cells. In: Journal of Virology. 1994 ; Vol. 68, No. 3. pp. 1615-1623.
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