Honokiol induces cell apoptosis in human chondrosarcoma cells through mitochondrial dysfunction and endoplasmic reticulum stress

Ying Ju Chen, Chien Lin Wu, Ju Fang Liu, Yi Chin Fong, Sheng Feng Hsu, Te Mao Li, Yi Chang Su, Shing Hwa Liu, Chih Hsin Tang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Chondrosarcoma is a malignant primary bone tumor that responds poorly to both chemotherapy and radiation therapy. In the present study, we investigated the anti-cancer effect of a honokiol, an active component isolated and purified from the Magnolia officinalis in human chondrosarcoma cells. Honokiol-induced cell apoptosis in human chondrosarcoma cell lines (including: JJ012 and SW1353) but not primary chondrocytes. Honokiol also induces upregulation of Bax and Bak, downregulation of Bcl-XL and dysfunction of mitochondria in chondrosarcoma cells. Honokiol triggered endoplasmic reticulum (ER) stress, as indicated by changes in cytosol-calcium levels. We also found that honokiol increased the expression and activities of glucose-regulated protein 78 (GRP78) and calpain. Transfection of cells with GRP78 or calpain siRNA reduced honokiol-mediated cell apoptosis in JJ012 cells. Importantly, animal studies have revealed a dramatic 53% reduction in tumor volume after 21 days of treatment. This study demonstrates that honokiol may be a novel anti-cancer agent targeting chondrosarcoma cells.

Original languageEnglish
Pages (from-to)20-30
Number of pages11
JournalCancer Letters
Volume291
Issue number1
DOIs
Publication statusPublished - May 1 2010

Keywords

  • Chondrosarcoma
  • ER
  • GRP78
  • Honokiol

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Honokiol induces cell apoptosis in human chondrosarcoma cells through mitochondrial dysfunction and endoplasmic reticulum stress'. Together they form a unique fingerprint.

  • Cite this