Honokiol-induced apoptosis and autophagy in glioblastoma multiforme cells

Ken Hu Chang, Ming De Yan, Chih Jung Yao, Pei Chun Lin, Gi Ming Lai

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Honokiol, a hydroxylated biphenyl compound isolated from the Chinese herb Magnolia officinalis, has been reported to have anticancer activities in a variety of cancer cell lines. The present study aimed to evaluate the anticancer effect and possible molecular mechanisms of honokiol in a glioblastoma multiforme (GBM) cell line. The anticancer activities of honokiol were investigated in the DBTRG-05MG GBM cell line. The effect of honokiol on cell growth was determined using a sulforhodamine B assay. Flow cytometry and immunoblotting were used to measure honokiol-induced apoptosis (programmed cell death type I) and autophagy (programmed cell death type II). Honokiol was observed to reduce DBTRG-05MG cell viability in a dose-dependent manner. At a dose of 50 μM, honokiol markedly decreased the expression of Rb protein and led to the cleavage of poly(ADP-ribose) polymerase and Bcl-xL to promote apoptosis in the cancer cells. In addition, markers of autophagy, including Beclin-1 and LC3-II, were also significantly increased. In addition to apoptosis, honokiol was also able to induce autophagy in the DBTRG-05MG cells. The mechanisms that are responsible for the correlation between honokiol-induced apoptosis and autophagy require further investigation. Such efforts may provide a potential strategy for improving the clinical outcome of GBM treatment.

Original languageEnglish
Pages (from-to)1435-1438
Number of pages4
JournalOncology Letters
Volume6
Issue number5
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Autophagy
Glioblastoma
Apoptosis
lissamine rhodamine B
Cell Line
Biphenyl Compounds
Magnolia
honokiol
Retinoblastoma Protein
Poly(ADP-ribose) Polymerases
Immunoblotting
Neoplasms
Cell Survival
Flow Cytometry
Growth

Keywords

  • Apoptosis
  • Autophagy
  • Glioblastoma multiforme
  • Honokiol

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Honokiol-induced apoptosis and autophagy in glioblastoma multiforme cells. / Chang, Ken Hu; Yan, Ming De; Yao, Chih Jung; Lin, Pei Chun; Lai, Gi Ming.

In: Oncology Letters, Vol. 6, No. 5, 11.2013, p. 1435-1438.

Research output: Contribution to journalArticle

Chang, Ken Hu ; Yan, Ming De ; Yao, Chih Jung ; Lin, Pei Chun ; Lai, Gi Ming. / Honokiol-induced apoptosis and autophagy in glioblastoma multiforme cells. In: Oncology Letters. 2013 ; Vol. 6, No. 5. pp. 1435-1438.
@article{a7e40427b2ef4ec0830002ca84f12680,
title = "Honokiol-induced apoptosis and autophagy in glioblastoma multiforme cells",
abstract = "Honokiol, a hydroxylated biphenyl compound isolated from the Chinese herb Magnolia officinalis, has been reported to have anticancer activities in a variety of cancer cell lines. The present study aimed to evaluate the anticancer effect and possible molecular mechanisms of honokiol in a glioblastoma multiforme (GBM) cell line. The anticancer activities of honokiol were investigated in the DBTRG-05MG GBM cell line. The effect of honokiol on cell growth was determined using a sulforhodamine B assay. Flow cytometry and immunoblotting were used to measure honokiol-induced apoptosis (programmed cell death type I) and autophagy (programmed cell death type II). Honokiol was observed to reduce DBTRG-05MG cell viability in a dose-dependent manner. At a dose of 50 μM, honokiol markedly decreased the expression of Rb protein and led to the cleavage of poly(ADP-ribose) polymerase and Bcl-xL to promote apoptosis in the cancer cells. In addition, markers of autophagy, including Beclin-1 and LC3-II, were also significantly increased. In addition to apoptosis, honokiol was also able to induce autophagy in the DBTRG-05MG cells. The mechanisms that are responsible for the correlation between honokiol-induced apoptosis and autophagy require further investigation. Such efforts may provide a potential strategy for improving the clinical outcome of GBM treatment.",
keywords = "Apoptosis, Autophagy, Glioblastoma multiforme, Honokiol",
author = "Chang, {Ken Hu} and Yan, {Ming De} and Yao, {Chih Jung} and Lin, {Pei Chun} and Lai, {Gi Ming}",
year = "2013",
month = "11",
doi = "10.3892/ol.2013.1548",
language = "English",
volume = "6",
pages = "1435--1438",
journal = "Oncology Letters",
issn = "1792-1074",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - Honokiol-induced apoptosis and autophagy in glioblastoma multiforme cells

AU - Chang, Ken Hu

AU - Yan, Ming De

AU - Yao, Chih Jung

AU - Lin, Pei Chun

AU - Lai, Gi Ming

PY - 2013/11

Y1 - 2013/11

N2 - Honokiol, a hydroxylated biphenyl compound isolated from the Chinese herb Magnolia officinalis, has been reported to have anticancer activities in a variety of cancer cell lines. The present study aimed to evaluate the anticancer effect and possible molecular mechanisms of honokiol in a glioblastoma multiforme (GBM) cell line. The anticancer activities of honokiol were investigated in the DBTRG-05MG GBM cell line. The effect of honokiol on cell growth was determined using a sulforhodamine B assay. Flow cytometry and immunoblotting were used to measure honokiol-induced apoptosis (programmed cell death type I) and autophagy (programmed cell death type II). Honokiol was observed to reduce DBTRG-05MG cell viability in a dose-dependent manner. At a dose of 50 μM, honokiol markedly decreased the expression of Rb protein and led to the cleavage of poly(ADP-ribose) polymerase and Bcl-xL to promote apoptosis in the cancer cells. In addition, markers of autophagy, including Beclin-1 and LC3-II, were also significantly increased. In addition to apoptosis, honokiol was also able to induce autophagy in the DBTRG-05MG cells. The mechanisms that are responsible for the correlation between honokiol-induced apoptosis and autophagy require further investigation. Such efforts may provide a potential strategy for improving the clinical outcome of GBM treatment.

AB - Honokiol, a hydroxylated biphenyl compound isolated from the Chinese herb Magnolia officinalis, has been reported to have anticancer activities in a variety of cancer cell lines. The present study aimed to evaluate the anticancer effect and possible molecular mechanisms of honokiol in a glioblastoma multiforme (GBM) cell line. The anticancer activities of honokiol were investigated in the DBTRG-05MG GBM cell line. The effect of honokiol on cell growth was determined using a sulforhodamine B assay. Flow cytometry and immunoblotting were used to measure honokiol-induced apoptosis (programmed cell death type I) and autophagy (programmed cell death type II). Honokiol was observed to reduce DBTRG-05MG cell viability in a dose-dependent manner. At a dose of 50 μM, honokiol markedly decreased the expression of Rb protein and led to the cleavage of poly(ADP-ribose) polymerase and Bcl-xL to promote apoptosis in the cancer cells. In addition, markers of autophagy, including Beclin-1 and LC3-II, were also significantly increased. In addition to apoptosis, honokiol was also able to induce autophagy in the DBTRG-05MG cells. The mechanisms that are responsible for the correlation between honokiol-induced apoptosis and autophagy require further investigation. Such efforts may provide a potential strategy for improving the clinical outcome of GBM treatment.

KW - Apoptosis

KW - Autophagy

KW - Glioblastoma multiforme

KW - Honokiol

UR - http://www.scopus.com/inward/record.url?scp=84884476892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884476892&partnerID=8YFLogxK

U2 - 10.3892/ol.2013.1548

DO - 10.3892/ol.2013.1548

M3 - Article

AN - SCOPUS:84884476892

VL - 6

SP - 1435

EP - 1438

JO - Oncology Letters

JF - Oncology Letters

SN - 1792-1074

IS - 5

ER -