hnRNP A1 interacts with the 5′ untranslated regions of enterovirus 71 and Sindbis virus RNA and is required for viral replication

Jing Yi Lin, Shin Ru Shih, Manjing Pan, Carol Li, Chia Fang Lue, Victor Stollar, Mei Ling Li

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is involved in pre-mRNA splicing in the nucleus and translational regulation in the cytoplasm. The cytoplasmic redistribution of hnRNP A1 is a regulated process during viral infection and cellular stress. Here we demonstrate that hnRNP A1 not only is an internal ribosome entry site (IRES) trans-acting factor that binds specifically to the 5′ untranslated region (UTR) of enterovirus 71 (EV71) and regulates IRES-dependent translation but also binds to the 5′ UTR of Sindbis virus (SV) and facilitates its translation. The cytoplasmic relocalization of hnRNP A1 in EV71-infected cells leads to the enhancement of EV71 IRES-mediated translation, and its function can be substituted by hnRNP A2, whereas the cytoplasmic relocalization of hnRNP A1 following SV infection enhances the SV translation, but this function cannot be replaced by hnRNP A2. Our study provides the first direct evidence that the cytoplasmic relocalization of hnRNP A1 controls not only the IRES-dependent but also non-IRES-dependent translation initiations of RNA viruses.

Original languageEnglish
Pages (from-to)6106-6114
Number of pages9
JournalJournal of Virology
Volume83
Issue number12
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Fingerprint

Sindbis virus
Heterogeneous-Nuclear Ribonucleoproteins
Sindbis Virus
ribonucleoproteins
Enterovirus
5' Untranslated Regions
5' untranslated regions
virus replication
RNA
ribosomes
translation (genetics)
Virus Diseases
varespladib methyl
Trans-Activators
RNA Viruses
RNA Precursors
Ribosomes
infection
Cytoplasm
cytoplasm

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

hnRNP A1 interacts with the 5′ untranslated regions of enterovirus 71 and Sindbis virus RNA and is required for viral replication. / Lin, Jing Yi; Shih, Shin Ru; Pan, Manjing; Li, Carol; Lue, Chia Fang; Stollar, Victor; Li, Mei Ling.

In: Journal of Virology, Vol. 83, No. 12, 06.2009, p. 6106-6114.

Research output: Contribution to journalArticle

Lin, Jing Yi ; Shih, Shin Ru ; Pan, Manjing ; Li, Carol ; Lue, Chia Fang ; Stollar, Victor ; Li, Mei Ling. / hnRNP A1 interacts with the 5′ untranslated regions of enterovirus 71 and Sindbis virus RNA and is required for viral replication. In: Journal of Virology. 2009 ; Vol. 83, No. 12. pp. 6106-6114.
@article{f9cf66eeaae843b09d3ac80c511247c4,
title = "hnRNP A1 interacts with the 5′ untranslated regions of enterovirus 71 and Sindbis virus RNA and is required for viral replication",
abstract = "Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is involved in pre-mRNA splicing in the nucleus and translational regulation in the cytoplasm. The cytoplasmic redistribution of hnRNP A1 is a regulated process during viral infection and cellular stress. Here we demonstrate that hnRNP A1 not only is an internal ribosome entry site (IRES) trans-acting factor that binds specifically to the 5′ untranslated region (UTR) of enterovirus 71 (EV71) and regulates IRES-dependent translation but also binds to the 5′ UTR of Sindbis virus (SV) and facilitates its translation. The cytoplasmic relocalization of hnRNP A1 in EV71-infected cells leads to the enhancement of EV71 IRES-mediated translation, and its function can be substituted by hnRNP A2, whereas the cytoplasmic relocalization of hnRNP A1 following SV infection enhances the SV translation, but this function cannot be replaced by hnRNP A2. Our study provides the first direct evidence that the cytoplasmic relocalization of hnRNP A1 controls not only the IRES-dependent but also non-IRES-dependent translation initiations of RNA viruses.",
author = "Lin, {Jing Yi} and Shih, {Shin Ru} and Manjing Pan and Carol Li and Lue, {Chia Fang} and Victor Stollar and Li, {Mei Ling}",
year = "2009",
month = "6",
doi = "10.1128/JVI.02476-08",
language = "English",
volume = "83",
pages = "6106--6114",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - hnRNP A1 interacts with the 5′ untranslated regions of enterovirus 71 and Sindbis virus RNA and is required for viral replication

AU - Lin, Jing Yi

AU - Shih, Shin Ru

AU - Pan, Manjing

AU - Li, Carol

AU - Lue, Chia Fang

AU - Stollar, Victor

AU - Li, Mei Ling

PY - 2009/6

Y1 - 2009/6

N2 - Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is involved in pre-mRNA splicing in the nucleus and translational regulation in the cytoplasm. The cytoplasmic redistribution of hnRNP A1 is a regulated process during viral infection and cellular stress. Here we demonstrate that hnRNP A1 not only is an internal ribosome entry site (IRES) trans-acting factor that binds specifically to the 5′ untranslated region (UTR) of enterovirus 71 (EV71) and regulates IRES-dependent translation but also binds to the 5′ UTR of Sindbis virus (SV) and facilitates its translation. The cytoplasmic relocalization of hnRNP A1 in EV71-infected cells leads to the enhancement of EV71 IRES-mediated translation, and its function can be substituted by hnRNP A2, whereas the cytoplasmic relocalization of hnRNP A1 following SV infection enhances the SV translation, but this function cannot be replaced by hnRNP A2. Our study provides the first direct evidence that the cytoplasmic relocalization of hnRNP A1 controls not only the IRES-dependent but also non-IRES-dependent translation initiations of RNA viruses.

AB - Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is involved in pre-mRNA splicing in the nucleus and translational regulation in the cytoplasm. The cytoplasmic redistribution of hnRNP A1 is a regulated process during viral infection and cellular stress. Here we demonstrate that hnRNP A1 not only is an internal ribosome entry site (IRES) trans-acting factor that binds specifically to the 5′ untranslated region (UTR) of enterovirus 71 (EV71) and regulates IRES-dependent translation but also binds to the 5′ UTR of Sindbis virus (SV) and facilitates its translation. The cytoplasmic relocalization of hnRNP A1 in EV71-infected cells leads to the enhancement of EV71 IRES-mediated translation, and its function can be substituted by hnRNP A2, whereas the cytoplasmic relocalization of hnRNP A1 following SV infection enhances the SV translation, but this function cannot be replaced by hnRNP A2. Our study provides the first direct evidence that the cytoplasmic relocalization of hnRNP A1 controls not only the IRES-dependent but also non-IRES-dependent translation initiations of RNA viruses.

UR - http://www.scopus.com/inward/record.url?scp=66149116780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66149116780&partnerID=8YFLogxK

U2 - 10.1128/JVI.02476-08

DO - 10.1128/JVI.02476-08

M3 - Article

VL - 83

SP - 6106

EP - 6114

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 12

ER -