HMGB-1 induces cell motility and α5β1 integrin expression in human chondrosarcoma cells

Chih Hsin Tang, Yun Ting Keng, Ju Fang Liu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. High mobility group box chromosomal protein 1 (HMGB)-1 is a widely studied, ubiquitous nuclear protein that is present in eukaryotic cells, and plays a crucial role in inflammatory response. However, the effects of HMGB-1 on human chondrosarcoma cells are largely unknown. In this study, we found that HMGB-1 increased the migration and the expression of α5β1 integrin in human chondrosarcoma cells. Transfection of cells with receptor for advanced glycation end products (RAGE) receptor siRNA reduced HMGB-1-induced cell migration and integrin expression. Activations of phosphatidylinositol 3-kinase (PI3K), Akt, and AP-1 pathways after HMGB-1 treatment were demonstrated, and HMGB-1-induced expression of integrin and migration activity was inhibited by the specific inhibitor and mutant of PI3K, Akt, and AP-1 cascades. Taken together, our results indicated that HMGB-1 enhances the migration of chondrosarcoma cells by increasing α5β1 integrin expression through the RAGE receptor/PI3K/Akt/c-Jun/AP-1 signal transduction pathway.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalCancer Letters
Volume322
Issue number1
DOIs
Publication statusPublished - Sep 1 2012

Keywords

  • Chondrosarcoma
  • HMGB-1
  • Integrin
  • Migration
  • RAGE

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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