HLA-A*33-B*58: 45-DRB1*03, a deduced probable human leukocyte antigen haplotype associated with a human leukocyte antigen low-incidence allele B*58:45 in Taiwanese unrelated hematopoietic bone marrow stem cell donors

Kuo Liang Yang, Reuy Ho Kao, Chin Lon Lin, Py Yu Lin

Research output: Contribution to journalArticle

Abstract

Objective: HLA-B*58:45 is a low-incidence allele in the human leukocyte antigen-B (HLA) locus. The aim of this study is to confirm the ethnicity of B*58:45 and its deduced probable HLA-associated haplotype in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A total of 40,000 healthy unrelated volunteer bone marrow stem cell donors (aged, 20-45 years) were tested using a sequence-based typing method. We confirmed the low-incidence allele B*58:45 in Taiwanese donors. Polymerase chain reaction was performed to amplify exon 2 and exon 3 of the HLA-A and HLA-B loci and exon 2 of the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced in both directions with the BigDye Terminator Cycle Sequencing Ready Reaction Kit according to the manufacturer's protocols. Results: The DNA sequence of B*58:45 is identical to B*58:01:01 in exon 2 and exon 3 except for residue 595, where G is changed to A (codon 175, GGG→AGG). The nucleotide replacement causes an amino acid change at codon 175 where G (glycine) of B*58:01:01 is replaced by R (arginine). We deduced the probable HLA haplotype associated with B*58:45 in Taiwanese donors to be A*33-B*58:45-DRB1*03. Conclusion: Information on this deduced probable HLA haplotype in association with the low-incidence B*58:45 allele is of value for HLA testing laboratories for reference purposes and can help bone marrow donor registries find compatible donors for patients with this uncommon HLA allele.

Original languageEnglish
Pages (from-to)71-73
Number of pages3
JournalTzu Chi Medical Journal
Volume27
Issue number2
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

Fingerprint

HLA Antigens
Bone Marrow Cells
Haplotypes
Stem Cells
Alleles
Tissue Donors
Incidence
Exons
Codon
Bone Marrow
Hematopoietic Stem Cells
Glycine
Arginine
Registries
Healthy Volunteers
Nucleotides
Amino Acids
Polymerase Chain Reaction

Keywords

  • Haplotype
  • Hematopoietic stem cell
  • Human leukocyte antigen
  • Sequence-based typing
  • Transplantation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{ee5dd8008a074548b7b9caead67a45e7,
title = "HLA-A*33-B*58: 45-DRB1*03, a deduced probable human leukocyte antigen haplotype associated with a human leukocyte antigen low-incidence allele B*58:45 in Taiwanese unrelated hematopoietic bone marrow stem cell donors",
abstract = "Objective: HLA-B*58:45 is a low-incidence allele in the human leukocyte antigen-B (HLA) locus. The aim of this study is to confirm the ethnicity of B*58:45 and its deduced probable HLA-associated haplotype in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A total of 40,000 healthy unrelated volunteer bone marrow stem cell donors (aged, 20-45 years) were tested using a sequence-based typing method. We confirmed the low-incidence allele B*58:45 in Taiwanese donors. Polymerase chain reaction was performed to amplify exon 2 and exon 3 of the HLA-A and HLA-B loci and exon 2 of the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced in both directions with the BigDye Terminator Cycle Sequencing Ready Reaction Kit according to the manufacturer's protocols. Results: The DNA sequence of B*58:45 is identical to B*58:01:01 in exon 2 and exon 3 except for residue 595, where G is changed to A (codon 175, GGG→AGG). The nucleotide replacement causes an amino acid change at codon 175 where G (glycine) of B*58:01:01 is replaced by R (arginine). We deduced the probable HLA haplotype associated with B*58:45 in Taiwanese donors to be A*33-B*58:45-DRB1*03. Conclusion: Information on this deduced probable HLA haplotype in association with the low-incidence B*58:45 allele is of value for HLA testing laboratories for reference purposes and can help bone marrow donor registries find compatible donors for patients with this uncommon HLA allele.",
keywords = "Haplotype, Hematopoietic stem cell, Human leukocyte antigen, Sequence-based typing, Transplantation",
author = "Yang, {Kuo Liang} and Kao, {Reuy Ho} and Lin, {Chin Lon} and Lin, {Py Yu}",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.tcmj.2015.01.004",
language = "English",
volume = "27",
pages = "71--73",
journal = "Tzu Chi Medical Journal",
issn = "1016-3190",
publisher = "財團法人中華民國佛教慈濟慈善事業基金會",
number = "2",

}

TY - JOUR

T1 - HLA-A*33-B*58

T2 - 45-DRB1*03, a deduced probable human leukocyte antigen haplotype associated with a human leukocyte antigen low-incidence allele B*58:45 in Taiwanese unrelated hematopoietic bone marrow stem cell donors

AU - Yang, Kuo Liang

AU - Kao, Reuy Ho

AU - Lin, Chin Lon

AU - Lin, Py Yu

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Objective: HLA-B*58:45 is a low-incidence allele in the human leukocyte antigen-B (HLA) locus. The aim of this study is to confirm the ethnicity of B*58:45 and its deduced probable HLA-associated haplotype in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A total of 40,000 healthy unrelated volunteer bone marrow stem cell donors (aged, 20-45 years) were tested using a sequence-based typing method. We confirmed the low-incidence allele B*58:45 in Taiwanese donors. Polymerase chain reaction was performed to amplify exon 2 and exon 3 of the HLA-A and HLA-B loci and exon 2 of the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced in both directions with the BigDye Terminator Cycle Sequencing Ready Reaction Kit according to the manufacturer's protocols. Results: The DNA sequence of B*58:45 is identical to B*58:01:01 in exon 2 and exon 3 except for residue 595, where G is changed to A (codon 175, GGG→AGG). The nucleotide replacement causes an amino acid change at codon 175 where G (glycine) of B*58:01:01 is replaced by R (arginine). We deduced the probable HLA haplotype associated with B*58:45 in Taiwanese donors to be A*33-B*58:45-DRB1*03. Conclusion: Information on this deduced probable HLA haplotype in association with the low-incidence B*58:45 allele is of value for HLA testing laboratories for reference purposes and can help bone marrow donor registries find compatible donors for patients with this uncommon HLA allele.

AB - Objective: HLA-B*58:45 is a low-incidence allele in the human leukocyte antigen-B (HLA) locus. The aim of this study is to confirm the ethnicity of B*58:45 and its deduced probable HLA-associated haplotype in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A total of 40,000 healthy unrelated volunteer bone marrow stem cell donors (aged, 20-45 years) were tested using a sequence-based typing method. We confirmed the low-incidence allele B*58:45 in Taiwanese donors. Polymerase chain reaction was performed to amplify exon 2 and exon 3 of the HLA-A and HLA-B loci and exon 2 of the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced in both directions with the BigDye Terminator Cycle Sequencing Ready Reaction Kit according to the manufacturer's protocols. Results: The DNA sequence of B*58:45 is identical to B*58:01:01 in exon 2 and exon 3 except for residue 595, where G is changed to A (codon 175, GGG→AGG). The nucleotide replacement causes an amino acid change at codon 175 where G (glycine) of B*58:01:01 is replaced by R (arginine). We deduced the probable HLA haplotype associated with B*58:45 in Taiwanese donors to be A*33-B*58:45-DRB1*03. Conclusion: Information on this deduced probable HLA haplotype in association with the low-incidence B*58:45 allele is of value for HLA testing laboratories for reference purposes and can help bone marrow donor registries find compatible donors for patients with this uncommon HLA allele.

KW - Haplotype

KW - Hematopoietic stem cell

KW - Human leukocyte antigen

KW - Sequence-based typing

KW - Transplantation

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U2 - 10.1016/j.tcmj.2015.01.004

DO - 10.1016/j.tcmj.2015.01.004

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JO - Tzu Chi Medical Journal

JF - Tzu Chi Medical Journal

SN - 1016-3190

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