Highly sensitive color-indicating and quantitative biosensor based on cholesteric liquid crystal

Yu Cheng Hsiao, Yu Chien Sung, Mon Juan Lee, Wei Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Liquid crystal (LC)-based biosensors employ highly sensitive interfaces between the alignment layers and LCs to detect biomolecules and their interactions. Present techniques based on optical texture observation of the homeotropic-to-planar response of nematic LCs are limited by their quantitative reproducibility of results, indicating that both the accuracy and reliability of LC-based detection require further improvements. Here we show that cholesteric LC (CLC) can be used as a novel sensing element in the design of an alternative LC-based biosensing device. The chirality of the vertically anchored (VA) CLC was exploited in the detection of bovine serum albumin (BSA), a protein standard commonly used in protein quantitation. The color appearance and the corresponding transmission spectrum of the cholesteric phase changed with the concentration of BSA, by which a detection limit of 1 fg/ml was observed. The optical response of the VA CLC interface offers a simple and inexpensive platform for highly sensitive and naked-eye color-indicating detection of biomolecules, and, thus, may facilitate the development of point-of-care devices for the detection of disease-related biomarkers.

Original languageEnglish
Article number250493
Pages (from-to)5033-5038
Number of pages6
JournalBiomedical Optics Express
Volume6
Issue number12
DOIs
Publication statusPublished - Nov 23 2015
Externally publishedYes

Fingerprint

Liquid Crystals
Biosensing Techniques
bioinstrumentation
Color
liquid crystals
color
Bovine Serum Albumin
Point-of-Care Systems
Eye Color
albumins
Equipment and Supplies
serums
Reproducibility of Results
proteins
Limit of Detection
Proteins
biomarkers
Biomarkers
Observation
chirality

Keywords

  • Chiral media
  • Liquid crystals
  • Liquid-crystal devices
  • Medical optics and biotechnology

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Highly sensitive color-indicating and quantitative biosensor based on cholesteric liquid crystal. / Hsiao, Yu Cheng; Sung, Yu Chien; Lee, Mon Juan; Lee, Wei.

In: Biomedical Optics Express, Vol. 6, No. 12, 250493, 23.11.2015, p. 5033-5038.

Research output: Contribution to journalArticle

Hsiao, Yu Cheng ; Sung, Yu Chien ; Lee, Mon Juan ; Lee, Wei. / Highly sensitive color-indicating and quantitative biosensor based on cholesteric liquid crystal. In: Biomedical Optics Express. 2015 ; Vol. 6, No. 12. pp. 5033-5038.
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