Abstract
This work demonstrates a strategy of double recognition hybrid aptamer–molecular imprinted polymer/quantum dots (apt–MIP/QDs) fluorescent sensor for specific recognition of target protein. We selected vinyl modified Mn–doped ZnS QDs as signal source and support. Instead of relying on single source of weak interaction between protein and small molecule monomer, thiol terminated aptamers were used as specific functional monomers to obtain apt–MIP on the surface of QDs. The double recognition was based on aptamer and imprinted cavity to overcome the challenges of protein imprinting. The synergistic effect of the aptamer and MAA contributed for highly specific recognition ability of the apt–MIP/QDs sensor toward Cyt C. The imprinting factor and the selectivity factor values of the hybrid apt–MIP/QDs sensor were 3.17 and 8.33, respectively. This composite sensor showed an excellent linear correlation for Cyt C in the concentration range of 0.20–2.00 μM. The detection limit for Cyt C was 0.054 μM A sensitive, rapid and convenient recognition system for Cyt C in biological samples was developed. The recoveries were between 94.2% and 102.5% for urine and serum samples.
| Original language | English |
|---|---|
| Pages (from-to) | 627-635 |
| Number of pages | 9 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 274 |
| DOIs | |
| Publication status | Published - Nov 20 2018 |
| Externally published | Yes |
Fingerprint
Keywords
- Aptamer
- Fluorescent sensor
- Molecular imprinting
- Quantum dots
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry
Cite this
Highly efficient fluorescent QDs sensor for specific detection of protein through double recognition of hybrid aptamer-molecular imprinted polymers. / Tan, Jiean; Guo, Manli; Tan, Lei; Geng, Yuanyuan; Huang, Shuyi; Tang, Youwen; Su, Chaochin; Lin, Chun Che; Liang, Yong.
In: Sensors and Actuators, B: Chemical, Vol. 274, 20.11.2018, p. 627-635.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Highly efficient fluorescent QDs sensor for specific detection of protein through double recognition of hybrid aptamer-molecular imprinted polymers
AU - Tan, Jiean
AU - Guo, Manli
AU - Tan, Lei
AU - Geng, Yuanyuan
AU - Huang, Shuyi
AU - Tang, Youwen
AU - Su, Chaochin
AU - Lin, Chun Che
AU - Liang, Yong
PY - 2018/11/20
Y1 - 2018/11/20
N2 - This work demonstrates a strategy of double recognition hybrid aptamer–molecular imprinted polymer/quantum dots (apt–MIP/QDs) fluorescent sensor for specific recognition of target protein. We selected vinyl modified Mn–doped ZnS QDs as signal source and support. Instead of relying on single source of weak interaction between protein and small molecule monomer, thiol terminated aptamers were used as specific functional monomers to obtain apt–MIP on the surface of QDs. The double recognition was based on aptamer and imprinted cavity to overcome the challenges of protein imprinting. The synergistic effect of the aptamer and MAA contributed for highly specific recognition ability of the apt–MIP/QDs sensor toward Cyt C. The imprinting factor and the selectivity factor values of the hybrid apt–MIP/QDs sensor were 3.17 and 8.33, respectively. This composite sensor showed an excellent linear correlation for Cyt C in the concentration range of 0.20–2.00 μM. The detection limit for Cyt C was 0.054 μM A sensitive, rapid and convenient recognition system for Cyt C in biological samples was developed. The recoveries were between 94.2% and 102.5% for urine and serum samples.
AB - This work demonstrates a strategy of double recognition hybrid aptamer–molecular imprinted polymer/quantum dots (apt–MIP/QDs) fluorescent sensor for specific recognition of target protein. We selected vinyl modified Mn–doped ZnS QDs as signal source and support. Instead of relying on single source of weak interaction between protein and small molecule monomer, thiol terminated aptamers were used as specific functional monomers to obtain apt–MIP on the surface of QDs. The double recognition was based on aptamer and imprinted cavity to overcome the challenges of protein imprinting. The synergistic effect of the aptamer and MAA contributed for highly specific recognition ability of the apt–MIP/QDs sensor toward Cyt C. The imprinting factor and the selectivity factor values of the hybrid apt–MIP/QDs sensor were 3.17 and 8.33, respectively. This composite sensor showed an excellent linear correlation for Cyt C in the concentration range of 0.20–2.00 μM. The detection limit for Cyt C was 0.054 μM A sensitive, rapid and convenient recognition system for Cyt C in biological samples was developed. The recoveries were between 94.2% and 102.5% for urine and serum samples.
KW - Aptamer
KW - Fluorescent sensor
KW - Molecular imprinting
KW - Quantum dots
UR - http://www.scopus.com/inward/record.url?scp=85051126762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051126762&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2018.07.126
DO - 10.1016/j.snb.2018.07.126
M3 - Article
AN - SCOPUS:85051126762
VL - 274
SP - 627
EP - 635
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -