Highly Effective Photocontrollable Drug Delivery Systems Based on Ultrasensitive Light-Responsive Self-Assembled Polymeric Micelles: An in Vitro Therapeutic Evaluation

Chih Chia Cheng, Jyun Jie Huang, Ai Wei Lee, Shan You Huang, Chien Yu Huang, Juin Yih Lai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An ultrasensitive light-responsive block copolymer, a combination of a multiarmed poly(ethylene glycol)-b-poly(caprolactone) polymer as a water-soluble element and maleimide-anthracene linkers as a photosensitive group, was successfully synthesized and rapidly self-assembled to form spherical micellar nanoparticles in aqueous media and phosphate-buffered saline. Their unique characteristics, such as extremely low critical micelle concentration, desirable micellar stability, well-controlled light-responsiveness, tailorable drug-loading content, and ultrasensitive light-induced drug release, make these micelles potential candidates for development of a more effective, safer drug delivery platform for cancer treatment. In vitro studies revealed that the drug-loaded micelles exhibited high structural stability in serum-containing media and very low toxicity toward normal and cancer cells under physiological conditions. Irradiation of cancer cells incubated with the drug-loaded micelles with ultraviolet light at 254 nm for only 10 s triggered rapid and complete release of the drug in the intracellular environment and induced strong antiproliferative/cytotoxic activity. Importantly, real-time cytotoxic assays and fluorescence imaging analysis further demonstrated that the drug-loaded micelles were rapidly taken up into the cytosol or nuclei of the cells, and subsequent ultraviolet exposure induced drug release and apoptotic cell death. Given their simplicity of design, high reliability, and performance, this new light-sensitive micelle may provide a promising route for developing a multifunctional therapeutic nanocarrier system.

Original languageEnglish
Pages (from-to)2162-2170
Number of pages9
JournalACS Applied Bio Materials
Volume2
Issue number5
DOIs
Publication statusPublished - May 20 2019

Keywords

  • controlled drug delivery
  • photoresponsive micelles
  • polymer assembly
  • real-time cytotoxicity assays
  • water-soluble functional block copolymer

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

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