High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field

Yu Nan Lin, Peng Chun Chen, Ren Guei Wu, Li Chern Pan, Fan Gang Tseng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we propose a microfluidic device for differentiating sperm motilities by interacting sperm's flowing upstream property with dual gradient flow field. Non-motile sperms will be flushed out of the second acceleration flow field while the motile sperms will be separated by the first gradient flow field with decreasing flow speed according to their motilities. This gentle sperm sorting process can accumulate millions of motile sperms from billions of sperms in half an hour, and categorized them into 3 different levels, suitable for in vitro fertilization (IVF) and artificial insemination (AI) applications.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages645-647
Number of pages3
Volume1
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Sorting
Flow fields
Throughput
Microfluidics

ASJC Scopus subject areas

  • Bioengineering

Cite this

Lin, Y. N., Chen, P. C., Wu, R. G., Pan, L. C., & Tseng, F. G. (2013). High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 645-647). Chemical and Biological Microsystems Society.

High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field. / Lin, Yu Nan; Chen, Peng Chun; Wu, Ren Guei; Pan, Li Chern; Tseng, Fan Gang.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 645-647.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lin, YN, Chen, PC, Wu, RG, Pan, LC & Tseng, FG 2013, High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 645-647, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Lin YN, Chen PC, Wu RG, Pan LC, Tseng FG. High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1. Chemical and Biological Microsystems Society. 2013. p. 645-647
Lin, Yu Nan ; Chen, Peng Chun ; Wu, Ren Guei ; Pan, Li Chern ; Tseng, Fan Gang. / High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. pp. 645-647
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