High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field

Yu Nan Lin; Peng Chun Chen; Ren Guei Wu; Li Chern Pan; Fan Gang Tseng

High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field

Yu Nan Lin, Peng Chun Chen, Ren Guei Wu, Li Chern Pan, Fan Gang Tseng

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this study, we propose a microfluidic device for differentiating sperm motilities by interacting sperm's flowing upstream property with dual gradient flow field. Non-motile sperms will be flushed out of the second acceleration flow field while the motile sperms will be separated by the first gradient flow field with decreasing flow speed according to their motilities. This gentle sperm sorting process can accumulate millions of motile sperms from billions of sperms in half an hour, and categorized them into 3 different levels, suitable for in vitro fertilization (IVF) and artificial insemination (AI) applications.

Original language	English
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Publisher	Chemical and Biological Microsystems Society
Pages	645-647
Number of pages	3
Volume	1
ISBN (Print)	9781632666246
Publication status	Published - 2013
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: Oct 27 2013 → Oct 31 2013

Other

Other	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country	Germany
City	Freiburg
Period	10/27/13 → 10/31/13

ASJC Scopus subject areas

Bioengineering

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High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field. / Lin, Yu Nan; Chen, Peng Chun; Wu, Ren Guei; Pan, Li Chern; Tseng, Fan Gang.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 645-647.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lin, YN, Chen, PC, Wu, RG, Pan, LC & Tseng, FG 2013, High-throughput sperm sorting by sperm flowing upstream in a dual gradient flow field. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 645-647, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.

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