High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens

Yu Jui Fan, Yu Chun Kung, Yi Chien Wu, Kuo Wei Huang, Ting Hsiang Wu, Yue Chen, Horn Jiunn Sheen, Pei Yu Chiou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We report a high throughput microfluidic fluorescence based flow cytometer with parallel sheath flow focusing in 32 channels using only 2 inlets. A detection throughput of 7000 beads/sec in each channel and 224,000 beads/sec for 32 channels has been accomplished. This fluorescence flow cytometer is realized by utilizing 3D soft lithography microfabrication technique to achieve multilayer PDMS structures with vertical vias connecting fluid in different layers. An array of high N.A. microball lens is also embedded in the structure to achieve parallel and high sensitivity fluorescence detection across these 32 channels.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1909-1911
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Fingerprint

Microfluidics
Fluorescence
Throughput
Microfabrication
Lithography
Lenses
Multilayers
Fluids

Keywords

  • 3D microfluidics
  • FACS
  • Flow cytometry
  • Microlens

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Fan, Y. J., Kung, Y. C., Wu, Y. C., Huang, K. W., Wu, T. H., Chen, Y., ... Chiou, P. Y. (2012). High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1909-1911). Chemical and Biological Microsystems Society.

High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. / Fan, Yu Jui; Kung, Yu Chun; Wu, Yi Chien; Huang, Kuo Wei; Wu, Ting Hsiang; Chen, Yue; Sheen, Horn Jiunn; Chiou, Pei Yu.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1909-1911.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fan, YJ, Kung, YC, Wu, YC, Huang, KW, Wu, TH, Chen, Y, Sheen, HJ & Chiou, PY 2012, High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1909-1911, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
Fan YJ, Kung YC, Wu YC, Huang KW, Wu TH, Chen Y et al. High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1909-1911
Fan, Yu Jui ; Kung, Yu Chun ; Wu, Yi Chien ; Huang, Kuo Wei ; Wu, Ting Hsiang ; Chen, Yue ; Sheen, Horn Jiunn ; Chiou, Pei Yu. / High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1909-1911
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