High-performance sample substrate of gold nanoparticle multilayers for surface-assisted laser desorption/ionization mass spectrometry

Yen Chen Liu, Yi Hsuan Chang, Yun Ho Lin, Chien Chung Liou, Tsung Rong Kuo

Research output: Contribution to journalArticle

Abstract

The development of a sample substrate with superior performance for desorption and ionization of analyte is the key issue to ameliorate the quality of mass spectra for measurements of small molecules in surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Herein, the homogeneous sample substrate of gold nanoparticle multilayers (AuNPs-ML) with hexagonal lattice was successfully prepared by self-assembly technique. With strong surface plasmon resonance absorption and superior photothermal effect, the sample substrate of AuNPs-ML exhibited high signal sensitivity and low background noise for the detection of model analyte of glucose without additional matrixes in SALDI-MS. Furthermore, compared to merchant matrixes of α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB), the sample substrate of AuNPs-ML was demonstrated to ameliorate the quality of mass spectra, including signal strength, background interference and signal/noise (S/N) ratio. The sucrose and tryptophan were also measured to show the extensive applications of AuNPs-ML sample substrate for the detections of small molecules in SALDI-MS. Most importantly, the remarkable reproducibility of glucose mass spectra with relative signal of 7.3% was obtained by the use of AuNPs-ML sample substrate for SALDI-MS. The homogeneous sample substrate of AuNPs-ML greatly improved the quality of mass spectra because of its strong absorption of laser energy, low specific heat, high heat conductivity and extraordinary homogeneity. We believe that AuNPs-ML could be a practical sample substrate for small molecule detection in SALDI-MS.

Original languageEnglish
Article number1078
JournalNanomaterials
Volume9
Issue number8
DOIs
Publication statusPublished - Jul 1 2019

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Keywords

  • Gold nanoparticle multilayers
  • Gold nanoparticles
  • Photothermal effect
  • Reproducibility
  • Sample substrate
  • Self-assembly

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

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