High performance liquid chromatography analysis of tetrahydrozoline hydrochloride in ophthalmic solution by silica column eluted with aqueous solvent mixtures

Ming Chuan Huang, Hsiu O. Ho, Kuo Ching Wen, Ming Thau Sheu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A HPLC method using bared silica column eluted with aqueous solvent mobile phase was developed for determination of tetrahydrozoline hydrochloride in ophthalmic preparations. A mixture of methanol and water (70:30, v/v) containing 0.03% triethylamine and 0.02% acetic acid was used as mobile phase, and chlorpheniramine maleate as an internal standard. The flow rate was 1 mL/min and the detection was at 254 nm. The quantitation limit was 1.0, μg/mL. Average recoveries range from 98.9 to 99.9%. The linearity of the calibration curve of tetrahydrozoline hydrochloride was well correlated (r 2 = 0.9996) within the range from 12.5 to 500 μg/mL as well as from 1.0 to 20 μg/mL (r 2 = 0.9997). This study further reports a simple and quick method for routine quantitative analysis of tetrahydrozoline hydrochloride in an ophthalmic solution that contains a relatively high concentration of sulfamethoxazole sodium and methyl paraben.

Original languageEnglish
Pages (from-to)88-94
Number of pages7
JournalJournal of Food and Drug Analysis
Volume10
Issue number2
Publication statusPublished - 2002

Fingerprint

Ophthalmic Solutions
Silicon Dioxide
silica
high performance liquid chromatography
eyes
High Pressure Liquid Chromatography
triethylamine
maleates
sulfamethoxazole
Parabens
Chlorpheniramine
acetic acid
quantitative analysis
Sulfamethoxazole
methanol
calibration
water
sodium
Acetic Acid
Calibration

Keywords

  • Analysis
  • HPLC
  • Ophthalmic solution
  • Silica column
  • Tetrahydrozoline

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

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title = "High performance liquid chromatography analysis of tetrahydrozoline hydrochloride in ophthalmic solution by silica column eluted with aqueous solvent mixtures",
abstract = "A HPLC method using bared silica column eluted with aqueous solvent mobile phase was developed for determination of tetrahydrozoline hydrochloride in ophthalmic preparations. A mixture of methanol and water (70:30, v/v) containing 0.03{\%} triethylamine and 0.02{\%} acetic acid was used as mobile phase, and chlorpheniramine maleate as an internal standard. The flow rate was 1 mL/min and the detection was at 254 nm. The quantitation limit was 1.0, μg/mL. Average recoveries range from 98.9 to 99.9{\%}. The linearity of the calibration curve of tetrahydrozoline hydrochloride was well correlated (r 2 = 0.9996) within the range from 12.5 to 500 μg/mL as well as from 1.0 to 20 μg/mL (r 2 = 0.9997). This study further reports a simple and quick method for routine quantitative analysis of tetrahydrozoline hydrochloride in an ophthalmic solution that contains a relatively high concentration of sulfamethoxazole sodium and methyl paraben.",
keywords = "Analysis, HPLC, Ophthalmic solution, Silica column, Tetrahydrozoline",
author = "Huang, {Ming Chuan} and Ho, {Hsiu O.} and Wen, {Kuo Ching} and Sheu, {Ming Thau}",
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journal = "Journal of Food and Drug Analysis",
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TY - JOUR

T1 - High performance liquid chromatography analysis of tetrahydrozoline hydrochloride in ophthalmic solution by silica column eluted with aqueous solvent mixtures

AU - Huang, Ming Chuan

AU - Ho, Hsiu O.

AU - Wen, Kuo Ching

AU - Sheu, Ming Thau

PY - 2002

Y1 - 2002

N2 - A HPLC method using bared silica column eluted with aqueous solvent mobile phase was developed for determination of tetrahydrozoline hydrochloride in ophthalmic preparations. A mixture of methanol and water (70:30, v/v) containing 0.03% triethylamine and 0.02% acetic acid was used as mobile phase, and chlorpheniramine maleate as an internal standard. The flow rate was 1 mL/min and the detection was at 254 nm. The quantitation limit was 1.0, μg/mL. Average recoveries range from 98.9 to 99.9%. The linearity of the calibration curve of tetrahydrozoline hydrochloride was well correlated (r 2 = 0.9996) within the range from 12.5 to 500 μg/mL as well as from 1.0 to 20 μg/mL (r 2 = 0.9997). This study further reports a simple and quick method for routine quantitative analysis of tetrahydrozoline hydrochloride in an ophthalmic solution that contains a relatively high concentration of sulfamethoxazole sodium and methyl paraben.

AB - A HPLC method using bared silica column eluted with aqueous solvent mobile phase was developed for determination of tetrahydrozoline hydrochloride in ophthalmic preparations. A mixture of methanol and water (70:30, v/v) containing 0.03% triethylamine and 0.02% acetic acid was used as mobile phase, and chlorpheniramine maleate as an internal standard. The flow rate was 1 mL/min and the detection was at 254 nm. The quantitation limit was 1.0, μg/mL. Average recoveries range from 98.9 to 99.9%. The linearity of the calibration curve of tetrahydrozoline hydrochloride was well correlated (r 2 = 0.9996) within the range from 12.5 to 500 μg/mL as well as from 1.0 to 20 μg/mL (r 2 = 0.9997). This study further reports a simple and quick method for routine quantitative analysis of tetrahydrozoline hydrochloride in an ophthalmic solution that contains a relatively high concentration of sulfamethoxazole sodium and methyl paraben.

KW - Analysis

KW - HPLC

KW - Ophthalmic solution

KW - Silica column

KW - Tetrahydrozoline

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