High performance liquid chromatography analysis of tetrahydrozoline hydrochloride in ophthalmic solution by silica column eluted with aqueous solvent mixtures

Ming Chuan Huang, Hsiu O. Ho, Kuo Ching Wen, Ming Thau Sheu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A HPLC method using bared silica column eluted with aqueous solvent mobile phase was developed for determination of tetrahydrozoline hydrochloride in ophthalmic preparations. A mixture of methanol and water (70:30, v/v) containing 0.03% triethylamine and 0.02% acetic acid was used as mobile phase, and chlorpheniramine maleate as an internal standard. The flow rate was 1 mL/min and the detection was at 254 nm. The quantitation limit was 1.0, μg/mL. Average recoveries range from 98.9 to 99.9%. The linearity of the calibration curve of tetrahydrozoline hydrochloride was well correlated (r 2 = 0.9996) within the range from 12.5 to 500 μg/mL as well as from 1.0 to 20 μg/mL (r 2 = 0.9997). This study further reports a simple and quick method for routine quantitative analysis of tetrahydrozoline hydrochloride in an ophthalmic solution that contains a relatively high concentration of sulfamethoxazole sodium and methyl paraben.

Original languageEnglish
Pages (from-to)88-94
Number of pages7
JournalJournal of Food and Drug Analysis
Volume10
Issue number2
Publication statusPublished - 2002

Keywords

  • Analysis
  • HPLC
  • Ophthalmic solution
  • Silica column
  • Tetrahydrozoline

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

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