High glucose-induced apoptosis in human vascular endothelial cells is mediated through NF-κB and c-Jun NH2-terminal kinase pathway and prevented by PI3K/Akt/eNOS pathway

Feng Ming Ho, Wan-Wan Lin, Bing Chang Chen, Chien M. Chao, Chia Ron Yang, Lian Y. Lin, Chih Chang Lai, Shing H. Liu, Chiau S. Liau

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188 Citations (Scopus)


Our previous studies demonstrated that high glucose-induced apoptosis in human umbilical vein endothelial cells (HUVECs) is mediated by sequential activation of c-Jun N-terminal kinase (JNK) and caspase, and prevented by exogenous nitric oxide (NO). In this study we further elucidated the roles of the transcriptional factor NF-κB, phosphatidylinositol 3′-kinase (PI3K), Akt and endothelial nitric oxide synthase (eNOS) in the apoptosis of HUVECs induced by high glucose. The results showed that high glucose-induced apoptosis was significantly enhanced by PI3K inhibitors (wortmannin and LY294002), NOS inhibitor (NG-nitro-arginine methyl ester) and eNOS antisense oligonucleotide. In contrast, apoptosis was markedly reduced by NF-κB inhibitor (pyrrolidine dithiocarbamate, PDTC), NF-κB antisense oligonucleotide, NO donor (sodium nitroprusside, SNP), and overexpression of Akt. The high glucose-induced NF-κB activation and transient Akt phosphorylation were prevented by the presence of vitamin C. Moreover, high glucose-induced increase in eNOS expression was attenuated by PI3K inhibitors and the negative mutant of PI3K. The activity of JNK induced by high glucose was suppressed by NF-κB-specific antisense oligonucleotide. Taken together our results demonstrated that high glucose-induced HUVECs apoptosis is through NF-κB-dependent JNK activation and reactive oxygen species (ROS)-dependent Akt dephosphorylation. Activation of the ROS/PI3K/Akt/eNOS signaling pathway in early phase exerts protective effects against the induction of apoptosis by high glucose.

Original languageEnglish
Pages (from-to)391-399
Number of pages9
JournalCellular Signalling
Issue number3
Publication statusPublished - Mar 2006



  • Akt
  • Apoptosis
  • Endothelial cell
  • eNOS
  • High glucose
  • JNK
  • NF-κB
  • Reactive oxygen species

ASJC Scopus subject areas

  • Cell Biology

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