High-frequency ultrasound imaging to evaluate liver fibrosis progression in rats and yi guan jian herbal therapeutic effects

Wei Chen, Jiun Yu Chen, Yu Tang Tung, Hsiao Ling Chen, Chia Wen Kuo, Chia Hui Chuang, Kowit Yu Chong, Frank Chiahung Mao, Chuan Mu Chen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The animals used in liver fibrosis studies must usually be sacrificed. Ultrasound has been demonstrated to have the ability to diagnose hepatic fibrosis and cirrhosis in experimental small-animal models. However, few studies have used high-frequency ultrasound (HFU, 40 MHz) to monitor changes in the rat liver and other hollow organs longitudinally. In this study, liver fibrosis was induced by administering dimethylnitrosamine (DMN) in SD rats, aged 8 weeks, for three consecutive days per week for up to 4 weeks. A Chinese herbal medicine Yi Guan Jian (YGJ) was orally administered (1.8 g/kg daily) to DMN-induced liver fibrosis rats for 2 weeks. Compared with the normal control rats, rats treated with DMN for either 2 weeks or 4 weeks had significantly lower body weights, liver indexes and elevation of hydroxyproline, GOT, and GPT contents. YGJ herbal treatment remarkably prevented rats from DMN-induced liver fibrosis. The HFU scoring results among the normal controls, 2-week DMN-treated rats, 4-week DMN-treated rats, and combined 2-week YGJ therapy with 4-week DMN-treated rats also reached statistical significance. Thus, HFU is an accurate tool for the longitudinal analysis of liver fibrosis progression in small-animal models, and the YGJ may be useful in reversing the development of hepatic fibrosis.

Original languageEnglish
Article number302325
JournalEvidence-based Complementary and Alternative Medicine
Volume2013
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Therapeutic Uses
Dimethylnitrosamine
Liver Cirrhosis
Ultrasonography
Liver
Fibrosis
Animal Models
Herbal Medicine
Hydroxyproline
Body Weight

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

High-frequency ultrasound imaging to evaluate liver fibrosis progression in rats and yi guan jian herbal therapeutic effects. / Chen, Wei; Chen, Jiun Yu; Tung, Yu Tang; Chen, Hsiao Ling; Kuo, Chia Wen; Chuang, Chia Hui; Chong, Kowit Yu; Mao, Frank Chiahung; Chen, Chuan Mu.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2013, 302325, 2013.

Research output: Contribution to journalArticle

Chen, Wei ; Chen, Jiun Yu ; Tung, Yu Tang ; Chen, Hsiao Ling ; Kuo, Chia Wen ; Chuang, Chia Hui ; Chong, Kowit Yu ; Mao, Frank Chiahung ; Chen, Chuan Mu. / High-frequency ultrasound imaging to evaluate liver fibrosis progression in rats and yi guan jian herbal therapeutic effects. In: Evidence-based Complementary and Alternative Medicine. 2013 ; Vol. 2013.
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