High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site

Jin Ching Lee, Tzong Yuan Wu, Chien Fu Huang, Feng Mine Yang, Shin Ru Shih, John T.A. Hsu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.

Original languageEnglish
Pages (from-to)656-662
Number of pages7
JournalBiotechnology and Bioengineering
Volume90
Issue number5
DOIs
Publication statusPublished - Jun 5 2005
Externally publishedYes

Fingerprint

Recombinant Fusion Proteins
Genetic engineering
Viruses
Animals
RNA
Enzymes
Proteins
Cells
Alkaline Phosphatase
Messenger RNA
CHO Cells

Keywords

  • EMCV
  • EV71
  • HCV
  • IRES
  • Polycistronic
  • Protein expression

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site. / Lee, Jin Ching; Wu, Tzong Yuan; Huang, Chien Fu; Yang, Feng Mine; Shih, Shin Ru; Hsu, John T.A.

In: Biotechnology and Bioengineering, Vol. 90, No. 5, 05.06.2005, p. 656-662.

Research output: Contribution to journalArticle

Lee, Jin Ching ; Wu, Tzong Yuan ; Huang, Chien Fu ; Yang, Feng Mine ; Shih, Shin Ru ; Hsu, John T.A. / High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site. In: Biotechnology and Bioengineering. 2005 ; Vol. 90, No. 5. pp. 656-662.
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