High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site

Jin Ching Lee; Tzong Yuan Wu; Chien Fu Huang; Feng Mine Yang; Shin Ru Shih; John T.A. Hsu

doi:10.1002/bit.20440

High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site

Jin Ching Lee, Tzong Yuan Wu, Chien Fu Huang, Feng Mine Yang, Shin Ru Shih, John T.A. Hsu

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.

Original language	English
Pages (from-to)	656-662
Number of pages	7
Journal	Biotechnology and Bioengineering
Volume	90
Issue number	5
DOIs	https://doi.org/10.1002/bit.20440
Publication status	Published - Jun 5 2005
Externally published	Yes

Keywords

EMCV
EV71
HCV
IRES
Polycistronic
Protein expression

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/bit.20440

Cite this

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title = "High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site",

abstract = "An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.",

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author = "Lee, {Jin Ching} and Wu, {Tzong Yuan} and Huang, {Chien Fu} and Yang, {Feng Mine} and Shih, {Shin Ru} and Hsu, {John T.A.}",

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doi = "10.1002/bit.20440",

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T1 - High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site

AU - Lee, Jin Ching

AU - Wu, Tzong Yuan

AU - Huang, Chien Fu

AU - Yang, Feng Mine

AU - Shih, Shin Ru

AU - Hsu, John T.A.

PY - 2005/6/5

Y1 - 2005/6/5

N2 - An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.

AB - An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.

KW - EMCV

KW - EV71

KW - HCV

KW - IRES

KW - Polycistronic

KW - Protein expression

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JF - Biotechnology and Bioengineering

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High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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