High cyclooxygenase-2 expression in cervical adenocarcinomas

Yi Jen Chen, Liang Shun Wang, Peng Hui Wang, Chiung Ru Lai, Ming Shyen Yen, Heung Tat Ng, Chiou Chung Yuan

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Objective. The purpose of this study was to examine the relationships between cyclooxygenase-2 (COX-2) expression and prognostic factors in cervical carcinomas. Methods. We studied COX-2 expression in 53 women with cervical cancers, including 35 squamous cell carcinomas (SCCs), 1 adenosquamous cell carcinoma (ASCC), and 17 adenocarcinomas (ACs), using commercially available polyclonal antibodies on Formalin-fixed, paraffin-embedded tissues. Normal cervical tissues were obtained as from other patients with uterine myomas treated with a total hysterectomy (n = 16). The immunoreactivity was quantified using an immunohistochemical scoring system that approximates the use of an image analysis-based system. Results. Twenty-two cervical cancer tissues (41.5%), including 10 SCCs and 12 ACs, expressed COX-2 at a moderate to strong level, which significantly, differed from the negligible expression found in the control group of 16 normal cervical tissues (P = 0.001). Different cell types showed significantly different expression levels of COX-2 (SCC at 28.6% vs AC at 70.6%, P = 0.004). The presence of deep stromal invasion (n = 40) showed a significant inverse relationship to COX-2 expression (32.5% vs 69.2%, P = 0.02). The expression of COX-2 in well-differentiated carcinomas was significantly increased compared to that in moderately and poorly differentiated carcinomas (72.7% vs 33.3%, respectively, P = 0.018). Conclusions. Overexpression of COX-2 was found in both SCC and AC, but SCCs showed infrequent and low expression. These findings suggest that increased COX-2 expression may play an important role in cervical adenocarcinomas.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalGynecologic Oncology
Volume88
Issue number3
DOIs
Publication statusPublished - Mar 1 2003
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Adenocarcinoma
Squamous Cell Carcinoma
Carcinoma
Uterine Cervical Neoplasms
Adenosquamous Carcinoma
Myoma
Hysterectomy
Paraffin
Formaldehyde
Control Groups
Antibodies

Keywords

  • Adenocarcinoma
  • Cervical cancer
  • Cyclooxygenase (COX-2)
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

Cite this

Chen, Y. J., Wang, L. S., Wang, P. H., Lai, C. R., Yen, M. S., Ng, H. T., & Yuan, C. C. (2003). High cyclooxygenase-2 expression in cervical adenocarcinomas. Gynecologic Oncology, 88(3), 379-385. https://doi.org/10.1016/S0090-8258(02)00066-5

High cyclooxygenase-2 expression in cervical adenocarcinomas. / Chen, Yi Jen; Wang, Liang Shun; Wang, Peng Hui; Lai, Chiung Ru; Yen, Ming Shyen; Ng, Heung Tat; Yuan, Chiou Chung.

In: Gynecologic Oncology, Vol. 88, No. 3, 01.03.2003, p. 379-385.

Research output: Contribution to journalArticle

Chen, YJ, Wang, LS, Wang, PH, Lai, CR, Yen, MS, Ng, HT & Yuan, CC 2003, 'High cyclooxygenase-2 expression in cervical adenocarcinomas', Gynecologic Oncology, vol. 88, no. 3, pp. 379-385. https://doi.org/10.1016/S0090-8258(02)00066-5
Chen YJ, Wang LS, Wang PH, Lai CR, Yen MS, Ng HT et al. High cyclooxygenase-2 expression in cervical adenocarcinomas. Gynecologic Oncology. 2003 Mar 1;88(3):379-385. https://doi.org/10.1016/S0090-8258(02)00066-5
Chen, Yi Jen ; Wang, Liang Shun ; Wang, Peng Hui ; Lai, Chiung Ru ; Yen, Ming Shyen ; Ng, Heung Tat ; Yuan, Chiou Chung. / High cyclooxygenase-2 expression in cervical adenocarcinomas. In: Gynecologic Oncology. 2003 ; Vol. 88, No. 3. pp. 379-385.
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abstract = "Objective. The purpose of this study was to examine the relationships between cyclooxygenase-2 (COX-2) expression and prognostic factors in cervical carcinomas. Methods. We studied COX-2 expression in 53 women with cervical cancers, including 35 squamous cell carcinomas (SCCs), 1 adenosquamous cell carcinoma (ASCC), and 17 adenocarcinomas (ACs), using commercially available polyclonal antibodies on Formalin-fixed, paraffin-embedded tissues. Normal cervical tissues were obtained as from other patients with uterine myomas treated with a total hysterectomy (n = 16). The immunoreactivity was quantified using an immunohistochemical scoring system that approximates the use of an image analysis-based system. Results. Twenty-two cervical cancer tissues (41.5{\%}), including 10 SCCs and 12 ACs, expressed COX-2 at a moderate to strong level, which significantly, differed from the negligible expression found in the control group of 16 normal cervical tissues (P = 0.001). Different cell types showed significantly different expression levels of COX-2 (SCC at 28.6{\%} vs AC at 70.6{\%}, P = 0.004). The presence of deep stromal invasion (n = 40) showed a significant inverse relationship to COX-2 expression (32.5{\%} vs 69.2{\%}, P = 0.02). The expression of COX-2 in well-differentiated carcinomas was significantly increased compared to that in moderately and poorly differentiated carcinomas (72.7{\%} vs 33.3{\%}, respectively, P = 0.018). Conclusions. Overexpression of COX-2 was found in both SCC and AC, but SCCs showed infrequent and low expression. These findings suggest that increased COX-2 expression may play an important role in cervical adenocarcinomas.",
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AU - Chen, Yi Jen

AU - Wang, Liang Shun

AU - Wang, Peng Hui

AU - Lai, Chiung Ru

AU - Yen, Ming Shyen

AU - Ng, Heung Tat

AU - Yuan, Chiou Chung

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N2 - Objective. The purpose of this study was to examine the relationships between cyclooxygenase-2 (COX-2) expression and prognostic factors in cervical carcinomas. Methods. We studied COX-2 expression in 53 women with cervical cancers, including 35 squamous cell carcinomas (SCCs), 1 adenosquamous cell carcinoma (ASCC), and 17 adenocarcinomas (ACs), using commercially available polyclonal antibodies on Formalin-fixed, paraffin-embedded tissues. Normal cervical tissues were obtained as from other patients with uterine myomas treated with a total hysterectomy (n = 16). The immunoreactivity was quantified using an immunohistochemical scoring system that approximates the use of an image analysis-based system. Results. Twenty-two cervical cancer tissues (41.5%), including 10 SCCs and 12 ACs, expressed COX-2 at a moderate to strong level, which significantly, differed from the negligible expression found in the control group of 16 normal cervical tissues (P = 0.001). Different cell types showed significantly different expression levels of COX-2 (SCC at 28.6% vs AC at 70.6%, P = 0.004). The presence of deep stromal invasion (n = 40) showed a significant inverse relationship to COX-2 expression (32.5% vs 69.2%, P = 0.02). The expression of COX-2 in well-differentiated carcinomas was significantly increased compared to that in moderately and poorly differentiated carcinomas (72.7% vs 33.3%, respectively, P = 0.018). Conclusions. Overexpression of COX-2 was found in both SCC and AC, but SCCs showed infrequent and low expression. These findings suggest that increased COX-2 expression may play an important role in cervical adenocarcinomas.

AB - Objective. The purpose of this study was to examine the relationships between cyclooxygenase-2 (COX-2) expression and prognostic factors in cervical carcinomas. Methods. We studied COX-2 expression in 53 women with cervical cancers, including 35 squamous cell carcinomas (SCCs), 1 adenosquamous cell carcinoma (ASCC), and 17 adenocarcinomas (ACs), using commercially available polyclonal antibodies on Formalin-fixed, paraffin-embedded tissues. Normal cervical tissues were obtained as from other patients with uterine myomas treated with a total hysterectomy (n = 16). The immunoreactivity was quantified using an immunohistochemical scoring system that approximates the use of an image analysis-based system. Results. Twenty-two cervical cancer tissues (41.5%), including 10 SCCs and 12 ACs, expressed COX-2 at a moderate to strong level, which significantly, differed from the negligible expression found in the control group of 16 normal cervical tissues (P = 0.001). Different cell types showed significantly different expression levels of COX-2 (SCC at 28.6% vs AC at 70.6%, P = 0.004). The presence of deep stromal invasion (n = 40) showed a significant inverse relationship to COX-2 expression (32.5% vs 69.2%, P = 0.02). The expression of COX-2 in well-differentiated carcinomas was significantly increased compared to that in moderately and poorly differentiated carcinomas (72.7% vs 33.3%, respectively, P = 0.018). Conclusions. Overexpression of COX-2 was found in both SCC and AC, but SCCs showed infrequent and low expression. These findings suggest that increased COX-2 expression may play an important role in cervical adenocarcinomas.

KW - Adenocarcinoma

KW - Cervical cancer

KW - Cyclooxygenase (COX-2)

KW - Squamous cell carcinoma

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