High affinity interactions of coxsackievirus A9 with integrin αvβ3 (CD51/61) require the CYDMKTTC sequence of β3, but do not require the RGD sequence of the CAV-9 VP1 protein

Martha Triantafilou, Kathy Triantafilou, Keith M. Wilson, Yoshikazu Takada, Nelson Fernandez

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Integrins are transmembrane molecules involved in numerous cell matrix, cell-cell adhesion phenomena and also utilised as viral receptors. These interactions with integrins are mediated by brief oligopeptide recognition sequences. The Arg-Gly-Asp sequence (RGD), is recognized by many integrins, including integrin αvβ3 (CD51/61). Coxsackievirus A9 (CAV-9), a human pathogen that has an Arg-Gly-Asp sequence in the VP1 capsid protein, has been known to be one of the many viruses that utilise integrin αvβ3 as a receptor. In order to determine important binding sites of CAV-9 on integrin αvβ3, we performed binding studies of CAV-9 on CHO-αvβ3, CHO-αvβ1 and CHO-αvβ1-3-1 mutant cell line, in the presence of function blocking mAb specific for integrin αvβ3 and natural ligand vitronectin. Our experiments show that the CYDMKTTC sequence (187-193 residue) of integrin β3, which has been shown to be involved in ligand specificity, is an important binding site for CAV-9. We also report that an RGD-less Coxsackievirus A9 mutant can bind efficiently on the ligand binding site of integrin αvβ3. Thus documenting the capability of this RNA virus to interact with integrin αvβ3, without the presence of an Arg-Gly-Asp sequence. (C) 2000 American Society for Histocompatibility and Immunogenetics.

Original languageEnglish
Pages (from-to)453-459
Number of pages7
JournalHuman Immunology
Volume61
Issue number5
DOIs
Publication statusPublished - May 2000
Externally publishedYes

Keywords

  • β3
  • CAV-9
  • CD51/61
  • RGD

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Fingerprint Dive into the research topics of 'High affinity interactions of coxsackievirus A9 with integrin αvβ3 (CD51/61) require the CYDMKTTC sequence of β3, but do not require the RGD sequence of the CAV-9 VP1 protein'. Together they form a unique fingerprint.

  • Cite this