HIC1 and RassF1A methylation attenuates tubulin expression and cell stiffness in cancer

Chih Cheng Chen, Bo Ching He, Yao Li Chen, Kuan Der Lee, Chun Hsin Tung, Chia Chen Hsu, Ping Yi Lin, Pei Yi Chu, Yu Wei Leu, Wei En Fu, Shu Huei Hsiao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cell stiffness is a potential biomarker for monitoring cellular transformation, metastasis, and drug resistance development. Environmental factors relayed into the cell may result in formation of inheritable markers (e.g., DNA methylation), which provide selectable advantages (e.g., tumor development-favoring changes in cell stiffness). We previously demonstrated that targeted methylation of two tumor suppressor genes, hypermethylated in cancer 1 (HIC1) and Ras-association domain family member 1A (RassF1A), transformed mesenchymal stem cells (MSCs). Here, transformation-associated cytoskeleton and cell stiffness changes were evaluated. Atomic force microscopy (AFM) was used to detect cell stiffness, and immunostaining was used to measure cytoskeleton expression and distribution in cultured cells as well as in vivo. HIC1 and RassF1A methylation (me_HR)-transformed MSCs developed into tumors that clonally expanded in vivo. In me_HR-transformed MSCs, cell stiffness was lost, tubulin expression decreased, and F-actin was disorganized; DNA methylation inhibitor treatment suppressed their tumor progression, but did not fully restore their F-actin organization and stiffness. Thus, me_HR-induced cell transformation was accompanied by the loss of cellular stiffness, suggesting that somatic epigenetic changes provide inheritable selection markers during tumor propagation, but inhibition of oncogenic aberrant DNA methylation cannot restore cellular stiffness fully. Therefore, cell stiffness is a candidate biomarker for cells’ physiological status.

Original languageEnglish
Article number2884
JournalInternational Journal of Molecular Sciences
Volume19
Issue number10
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

methylation
Methylation
Tubulin
stiffness
cancer
Stiffness
Association reactions
cells
Neoplasms
stem cells
Tumors
DNA Methylation
tumors
Stem cells
Mesenchymal Stromal Cells
deoxyribonucleic acid
biomarkers
Biomarkers
Cytoskeleton
markers

Keywords

  • AFM
  • Cancer
  • DNA methylation
  • Stiffness

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

HIC1 and RassF1A methylation attenuates tubulin expression and cell stiffness in cancer. / Chen, Chih Cheng; He, Bo Ching; Chen, Yao Li; Lee, Kuan Der; Tung, Chun Hsin; Hsu, Chia Chen; Lin, Ping Yi; Chu, Pei Yi; Leu, Yu Wei; Fu, Wei En; Hsiao, Shu Huei.

In: International Journal of Molecular Sciences, Vol. 19, No. 10, 2884, 01.10.2018.

Research output: Contribution to journalArticle

Chen, CC, He, BC, Chen, YL, Lee, KD, Tung, CH, Hsu, CC, Lin, PY, Chu, PY, Leu, YW, Fu, WE & Hsiao, SH 2018, 'HIC1 and RassF1A methylation attenuates tubulin expression and cell stiffness in cancer', International Journal of Molecular Sciences, vol. 19, no. 10, 2884. https://doi.org/10.3390/ijms19102884
Chen, Chih Cheng ; He, Bo Ching ; Chen, Yao Li ; Lee, Kuan Der ; Tung, Chun Hsin ; Hsu, Chia Chen ; Lin, Ping Yi ; Chu, Pei Yi ; Leu, Yu Wei ; Fu, Wei En ; Hsiao, Shu Huei. / HIC1 and RassF1A methylation attenuates tubulin expression and cell stiffness in cancer. In: International Journal of Molecular Sciences. 2018 ; Vol. 19, No. 10.
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