Heterogeneity of myocardial sleeve morphology and gap junctions in canine superior vena cava

Hung I. Yeh, Yu Jun Lai, Shih Huang Lee, Yi Nan Lee, Yu Shien Ko, Shih Ann Chen, Nicholas J. Severs, Cheng Ho Tsai

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66 Citations (Scopus)

Abstract

Background - The myocardial sleeve of the superior vena cava (SVC) has been identified as a potential initiating focus in atrial fibrillation, but information on cell-to-cell linkage at this site is lacking. Methods and Results - We examined the SVC in 8 dogs by immunoconfocal and electron microscopy. Cardiomyocytes outlined with vinculin and bearing striations positive for α-actinin are found in the proximal segment of the SVC. These cells, grouped in bundles of various orientations according to location, extend cephalically as far as 3 cm from the right atrium (RA)-SVC junction. Comparison between the junctional level and the level 2 cm distal shows that the myocardial layer in the latter is thinner and not as compact and is composed of longer cells (87.3±15.7 versus 71.6±14.4 μm, P<0.01). Gap junctions made of connexin43 (Cx43), Cx40, and Cx45 are aggregated mainly at the intercalated disks, and colocalization of connexins is a common feature throughout the myocardial sleeve. Areas of atypical expression exist, however, characterized by a center of abundant Cx43 labels surrounded by a periphery of scattered tiny Cx40-labeled spots. Although in the ventral subluminal compact myocardial layer, individual cells at both levels are surrounded by similar numbers of cells, the number of aggregation of labeled gap junctions at the distal level is less (2.3±0.6 versus 3.7±0.9, P<0.01). In addition, electron -microscopic examination demonstrates that the gap junctions at the distal level are smaller in size (0.37±0.30 versus 0.55±0.34 μm, P<0.01). Conclusions - The myocardial sleeve in the canine SVC is a heterogeneous structure, which could potentially form a substrate for heterogeneity of electrical coupling.

Original languageEnglish
Pages (from-to)3152-3157
Number of pages6
JournalCirculation
Volume104
Issue number25
DOIs
Publication statusPublished - Dec 18 2001

Keywords

  • Junctions
  • Proteins
  • Veins

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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