Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation

Yi Kai Chen, Chih Hsiang Leng, Heidi Olivares, Ming Hui Lee, Yuan Chih Chang, Wen Mei Kung, Shih Chieh Ti, Yu Hui Lo, Andrew H.J. Wang, Chia Seng Chang, Douglas K. Bishop, Yi Ping Hsueh, Ting Fang Wang

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)

Abstract

Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosis-specific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.

Original languageEnglish
Pages (from-to)10572-10577
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number29
DOIs
Publication statusPublished - Jul 20 2004
Externally publishedYes

ASJC Scopus subject areas

  • General

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