Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators

Lie Fen Shyur, Chi Chang Huang, Chiu Ping Lo, Chih Yang Chiu, Yi Ping Chen, Sheng Yang Wang, Shang Tzen Chang

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection.

Original languageEnglish
Pages (from-to)1348-1358
Number of pages11
JournalPhytochemistry
Volume69
Issue number6
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

Fingerprint

Cryptomeria
Cryptomeria japonica
Liver
Modulators
Wood
Anti-Inflammatory Agents
Macrophages
Terpenes
prostaglandin synthase
Fractionation
Transaminases
Bioactivity
anti-inflammatory activity
Coniferophyta
Skin
mice
Chemical activation
Wounds and Injuries
wood extractives
hepatoprotective effect

Keywords

  • Cryptomeria japonica
  • Cycloxygenase-2
  • Heme oxygenase-1
  • Hepatoprotection
  • Inducible nitric oxide synthase
  • Nitric oxide
  • Taxodiaceae
  • Terpenoids

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators. / Shyur, Lie Fen; Huang, Chi Chang; Lo, Chiu Ping; Chiu, Chih Yang; Chen, Yi Ping; Wang, Sheng Yang; Chang, Shang Tzen.

In: Phytochemistry, Vol. 69, No. 6, 04.2008, p. 1348-1358.

Research output: Contribution to journalArticle

Shyur, Lie Fen ; Huang, Chi Chang ; Lo, Chiu Ping ; Chiu, Chih Yang ; Chen, Yi Ping ; Wang, Sheng Yang ; Chang, Shang Tzen. / Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators. In: Phytochemistry. 2008 ; Vol. 69, No. 6. pp. 1348-1358.
@article{949dc6153bda499184fa6cced8af8b25,
title = "Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators",
abstract = "Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection.",
keywords = "Cryptomeria japonica, Cycloxygenase-2, Heme oxygenase-1, Hepatoprotection, Inducible nitric oxide synthase, Nitric oxide, Taxodiaceae, Terpenoids",
author = "Shyur, {Lie Fen} and Huang, {Chi Chang} and Lo, {Chiu Ping} and Chiu, {Chih Yang} and Chen, {Yi Ping} and Wang, {Sheng Yang} and Chang, {Shang Tzen}",
year = "2008",
month = "4",
doi = "10.1016/j.phytochem.2008.01.017",
language = "English",
volume = "69",
pages = "1348--1358",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators

AU - Shyur, Lie Fen

AU - Huang, Chi Chang

AU - Lo, Chiu Ping

AU - Chiu, Chih Yang

AU - Chen, Yi Ping

AU - Wang, Sheng Yang

AU - Chang, Shang Tzen

PY - 2008/4

Y1 - 2008/4

N2 - Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection.

AB - Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection.

KW - Cryptomeria japonica

KW - Cycloxygenase-2

KW - Heme oxygenase-1

KW - Hepatoprotection

KW - Inducible nitric oxide synthase

KW - Nitric oxide

KW - Taxodiaceae

KW - Terpenoids

UR - http://www.scopus.com/inward/record.url?scp=41049090217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41049090217&partnerID=8YFLogxK

U2 - 10.1016/j.phytochem.2008.01.017

DO - 10.1016/j.phytochem.2008.01.017

M3 - Article

C2 - 18328512

AN - SCOPUS:41049090217

VL - 69

SP - 1348

EP - 1358

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - 6

ER -