Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators

Lie Fen Shyur, Chi Chang Huang, Chiu Ping Lo, Chih Yang Chiu, Yi Ping Chen, Sheng Yang Wang, Shang Tzen Chang

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection.

Original languageEnglish
Pages (from-to)1348-1358
Number of pages11
JournalPhytochemistry
Volume69
Issue number6
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

Keywords

  • Cryptomeria japonica
  • Cycloxygenase-2
  • Heme oxygenase-1
  • Hepatoprotection
  • Inducible nitric oxide synthase
  • Nitric oxide
  • Taxodiaceae
  • Terpenoids

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

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