Hepatoprotective effects of Ixora parviflora extract against exhaustive exercise-induced oxidative stress in mice

Nai Wen Kan, Wen Ching Huang, Wan Teng Lin, Chih Yang Huang, Kuo Ching Wen, Hsiu Mei Chiang, Chi Chang Huang, Mei Chich Hsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ixora parviflora, a species of the Rubiaceae, is rich in polyphenols and flavonoids, and has been traditionally used as a folk medicine. An I. parviflora extract (IPE) has great antioxidant activity in vitro, including a scavenging effect on superoxide radicals, reducing power, and ferrous ion-chelating ability. However, whether IPE is efficacious against oxidative damage in vivo is not known. The purpose of this study was to determine the protective effects of IPE treatment on hepatic oxidative stress and antioxidant defenses after exhaustive exercise in mice. Fifty male C57BL/6 mice (6 week old) were randomly divided into five groups and designated a sedentary control with vehicle (C), and exhaustive exercise with vehicle (IPE0), low dosage (IPE10), medium dosage (IPE50) and high dosage (IPE100) of IPE at 0, 10, 50, and 100 mg/kg, respectively. After a single bout of exhaustive swimming exercise challenge, levels of blood ammonia and creatine kinase (CK), and hepatic superoxide dismutase (SOD) protein expression, thiobarbituric acid-reactive substance (TBARS), and gp91phox, p22phox, and p47phox subunits of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase expressions in the IPE0 group were significantly affected compared to those of the C group, but they were all significantly inhibited by the IPE treatments. Results of the present in vivo study in mice indicate that I. parviflora extract possesses antioxidative and hepatoprotective potential following exhaustive exercise.

Original languageEnglish
Pages (from-to)10721-10732
Number of pages12
JournalMolecules
Volume18
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Oxidative stress
physical exercise
mice
Oxidative Stress
Antioxidants
Rubiaceae
Thiobarbituric Acid Reactive Substances
inorganic peroxides
Liver
Scavenging
antioxidants
Polyphenols
Traditional Medicine
Creatine Kinase
Chelation
NADP
Inbred C57BL Mouse
Flavonoids
Superoxides
dosage

Keywords

  • Exhaustive exercise
  • Ixora parviflora
  • Liver peroxidation
  • Oxidative stress

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Kan, N. W., Huang, W. C., Lin, W. T., Huang, C. Y., Wen, K. C., Chiang, H. M., ... Hsu, M. C. (2013). Hepatoprotective effects of Ixora parviflora extract against exhaustive exercise-induced oxidative stress in mice. Molecules, 18(9), 10721-10732. https://doi.org/10.3390/molecules180910721

Hepatoprotective effects of Ixora parviflora extract against exhaustive exercise-induced oxidative stress in mice. / Kan, Nai Wen; Huang, Wen Ching; Lin, Wan Teng; Huang, Chih Yang; Wen, Kuo Ching; Chiang, Hsiu Mei; Huang, Chi Chang; Hsu, Mei Chich.

In: Molecules, Vol. 18, No. 9, 09.2013, p. 10721-10732.

Research output: Contribution to journalArticle

Kan, NW, Huang, WC, Lin, WT, Huang, CY, Wen, KC, Chiang, HM, Huang, CC & Hsu, MC 2013, 'Hepatoprotective effects of Ixora parviflora extract against exhaustive exercise-induced oxidative stress in mice', Molecules, vol. 18, no. 9, pp. 10721-10732. https://doi.org/10.3390/molecules180910721
Kan, Nai Wen ; Huang, Wen Ching ; Lin, Wan Teng ; Huang, Chih Yang ; Wen, Kuo Ching ; Chiang, Hsiu Mei ; Huang, Chi Chang ; Hsu, Mei Chich. / Hepatoprotective effects of Ixora parviflora extract against exhaustive exercise-induced oxidative stress in mice. In: Molecules. 2013 ; Vol. 18, No. 9. pp. 10721-10732.
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