Hepatoprotective bioactivity of the glycoprotein, antrodan, isolated from Antrodia cinnamomea mycelia

Yaw Bee Ker, Chiung Chi Peng, Wan Lin Chang, Charng Cherng Chyau, Robert Y. Peng

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Antrodan, a protein-bound polysaccharide isolated from Antrodia cinnamomea mycelia, was demonstrated to exhibit significant anti-inflammatory bioactivity in vitro. However, its role in hepatic injury in vivo still remains unclear. We hypothesized that antrodan may have beneficial hepatoprotective effects. To verify this, a lipopolysaccharide (LPS)-Sprague-Dawley rat model was used. Antrodan protected against liver damage by suppressing LPS-stimulated serum glutamine-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), interleukin (IL)-6, hepatic thiobarbituric acid reactive substances (TBARS), nitric oxide (NO), inducible NO synthase (iNOS) and nuclear factor (NF)-κB, and by effectively alleviating the downregulated hepatic superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px). Hematoxylin-eosin staining revealed that antrodan at a dosage of 40 mg/kg was able to alleviate LPS-induced liver damage to a normal status. In addition, we identified the partial main architectural backbone of antrodan to have a 1→3 linear β-glycosidic backbone of mannan linked by β-1→3 glucosidic branches. Conclusively, antrodan can potentially ameliorate liver damage in vivo by suppressing oxidative stress induced by LPS.

Original languageEnglish
Article numbere93191
JournalPLoS One
Volume9
Issue number4
DOIs
Publication statusPublished - Apr 1 2014

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Antrodia
Taiwanofungus camphoratus
Mycelium
Bioactivity
mycelium
Lipopolysaccharides
glycoproteins
Glycoproteins
Liver
lipopolysaccharides
liver
glutamine-pyruvate aminotransferase
Mannans
glutathione peroxidase
Oxidative stress
Thiobarbituric Acid Reactive Substances
Nitric Oxide Synthase Type II
Hematoxylin
Eosine Yellowish-(YS)
Glutathione Peroxidase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Hepatoprotective bioactivity of the glycoprotein, antrodan, isolated from Antrodia cinnamomea mycelia. / Ker, Yaw Bee; Peng, Chiung Chi; Chang, Wan Lin; Chyau, Charng Cherng; Peng, Robert Y.

In: PLoS One, Vol. 9, No. 4, e93191, 01.04.2014.

Research output: Contribution to journalArticle

Ker, Yaw Bee ; Peng, Chiung Chi ; Chang, Wan Lin ; Chyau, Charng Cherng ; Peng, Robert Y. / Hepatoprotective bioactivity of the glycoprotein, antrodan, isolated from Antrodia cinnamomea mycelia. In: PLoS One. 2014 ; Vol. 9, No. 4.
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