Hepatitis C virus core protein induces neuroimmune activation and potentiates human immunodeficiency virus-1 neurotoxicity

Pornpun Vivithanaporn, Ferdinand Maingat, Liang Tzung Lin, Hong Na, Christopher D. Richardson, Babita Agrawal, Éric A. Cohen, Jack H. Jhamandas, Christopher Power

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Background: Hepatitis C virus (HCV) genomes and proteins are present in human brain tissues although the impact of HIV/ HCV co-infection on neuropathogenesis remains unclear. Herein, we investigate HCV infectivity and effects on neuronal survival and neuroinflammation in conjunction with HIV infection. Methodology: Human microglia, astrocyte and neuron cultures were infected with cell culture-derived HCV or exposed to HCV core protein with or without HIV-1 infection or HIV-1 Viral Protein R (Vpr) exposure. Host immune gene expression and cell viability were measured. Patch-clamp studies of human neurons were performed in the presence or absence of HCV core protein. Neurobehavioral performance and neuropathology were examined in HIV-1 Vpr-transgenic mice in which stereotaxic intrastriatal implants of HCV core protein were performed. Principal Findings: HCV-encoded RNA as well as HCV core and non-structural 3 (NS3) proteins were detectable in human microglia and astrocytes infected with HCV. HCV core protein exposure induced expression of pro-inflammatory cytokines including interleukin-1b, interleukin-6 and tumor necrosis factor-α in microglia (p

Original languageEnglish
Article numbere12856
Pages (from-to)1-14
Number of pages14
JournalPLoS One
Volume5
Issue number9
DOIs
Publication statusPublished - 2010
Externally publishedYes

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neurotoxicity
Hepatitis C virus
Human immunodeficiency virus 1
Viruses
Hepacivirus
HIV-1
Chemical activation
Human Immunodeficiency Virus vpr Gene Products
Microglia
vpr Gene Products
proteins
neuroglia
Cell culture
Astrocytes
Neurons
HIV Infections
viral proteins
astrocytes
Interleukins
Clamping devices

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Hepatitis C virus core protein induces neuroimmune activation and potentiates human immunodeficiency virus-1 neurotoxicity. / Vivithanaporn, Pornpun; Maingat, Ferdinand; Lin, Liang Tzung; Na, Hong; Richardson, Christopher D.; Agrawal, Babita; Cohen, Éric A.; Jhamandas, Jack H.; Power, Christopher.

In: PLoS One, Vol. 5, No. 9, e12856, 2010, p. 1-14.

Research output: Contribution to journalArticle

Vivithanaporn, P, Maingat, F, Lin, LT, Na, H, Richardson, CD, Agrawal, B, Cohen, ÉA, Jhamandas, JH & Power, C 2010, 'Hepatitis C virus core protein induces neuroimmune activation and potentiates human immunodeficiency virus-1 neurotoxicity', PLoS One, vol. 5, no. 9, e12856, pp. 1-14. https://doi.org/10.1371/journal.pone.0012856
Vivithanaporn, Pornpun ; Maingat, Ferdinand ; Lin, Liang Tzung ; Na, Hong ; Richardson, Christopher D. ; Agrawal, Babita ; Cohen, Éric A. ; Jhamandas, Jack H. ; Power, Christopher. / Hepatitis C virus core protein induces neuroimmune activation and potentiates human immunodeficiency virus-1 neurotoxicity. In: PLoS One. 2010 ; Vol. 5, No. 9. pp. 1-14.
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