Hemoglobin-Conjugated Gold Nanoclusters for Qualitative Analysis of Haptoglobin Phenotypes

Shih-Hua Tan, Sibidou Yougbaré, Hsueh-Liang Chu, Tsung-Rong Kuo, Tsai-Mu Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

Designing a facile and rapid detection method for haptoglobin (Hp) phenotypes in human blood plasma is urgently needed to meet clinic requirements in hemolysis theranostics. In this work, a novel approach to qualitatively analyze Hp phenotypes was developed using a fluorescent probe of gold nanoclusters (AuNCs). Hemoglobin-conjugated (Hb)-AuNCs were successfully synthesized with blue-green fluorescence and high biocompatibility via one-pot synthesis. The fluorescence of Hb-AuNCs comes from the ligand-metal charge transfer between surface ligands of Hb and the gold cores with high oxidation states. The biocompatibility assays including cell viability and fluorescence imaging, demonstrated high biocompatibility of Hb-AuNCs. For the qualitative analysis, three Hp phenotypes in plasma, Hp 1-1, Hp 2-1, and Hp 2-2, were successfully discriminated according to changes in the fluorescence intensity and peak position of the maximum intensity of Hb-AuNCs. Our work provides a practical method with facile and rapid properties for the qualitative analysis of three Hp phenotypes in human blood plasma.

Original languageEnglish
Article number2242
Pages (from-to)1-11
Number of pages11
JournalPolymers
Volume12
Issue number10
DOIs
Publication statusPublished - Oct 2020

Keywords

  • Gold nanoclusters
  • Haptoglobin
  • Hemoglobin
  • Phenotypes
  • Qualitative analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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