Helicobacter pylori cagA, iceA and vacA status in Taiwanese patients with peptic ulcer and gastritis

Chin Lin Perng, Hwai Jeng Lin, I. Chen Sun, Guan Ying Tseng

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Helicobacter pylori causes chronic gastritis, peptic ulcer, gastric cancer and mucosa-associated lymphoid tissue (MALT) lymphoma. Different genotypes of H. pylori are confirmed from diverse geographical areas. Its association with clinical diseases remains controversial. The aim of the present study was to investigate the H. pylori vacuolating cytotoxin (vacA) alleles, cytotoxin-associated gene (cagA) and iceA, in patients with peptic ulcer and gastritis. Methods: We enrolled patients with peptic ulcer and chronic gastritis. Biopsy specimens were obtained from the antrum and lower body of the stomach. DNA extraction and polymerase chain reaction (PCR) were used to detect the presence or absence of cagA and to assess the polymorphism of vacA and iceA. Results: A total of 133 patients (57 gastric ulcer, 52 duodenal ulcer, 24 chronic gastritis) had positive PCR results from biopsy specimens. Concerning genotypes, we found cagA (79% in the antrum, 92% in the body) and iceA1 (73% in the antrum, 82.8% in the body) strains in the majority of patients. The dominant vacA subtype was s1a (74.4% in the antrum, 75% in the body), followed by s1c (51.1% in the antrum, 60.5% in the body). In the middle region, the m2 strain dominated (49.6% in the antrum, 41.4% in the body), followed by m1T (19.5% in the antrum, 9.5% in the body). Mixed infection occurred in 89 patients (67%).There was no statistical difference in genotypes among the three groups. Conclusion: In Taiwan, H. pylori with positive cagA and iceA1 was found in the majority of cases. H. pylori with vacA s1a strains was the most common vacA subtype, followed by s1c, while s1b was rare. In the middle region, the m2 subtype was predominant followed by m1T. There was no significant association between genotypes and clinical diseases.

Original languageEnglish
Pages (from-to)1244-1249
Number of pages6
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume18
Issue number11
DOIs
Publication statusPublished - 2003
Externally publishedYes

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Gastritis
Peptic Ulcer
Helicobacter pylori
Genotype
Biopsy
Marginal Zone B-Cell Lymphoma
Polymerase Chain Reaction
Cytotoxins
DNA-Directed DNA Polymerase
Stomach Ulcer
Duodenal Ulcer
Gastric Mucosa
Coinfection
Taiwan
Stomach Neoplasms
Stomach
Alleles
Genes

Keywords

  • Cytotoxin-associated gene (cagA)
  • Genotypes
  • Helicobacter pylori
  • iceA
  • Vacuolating cytotoxin (vacA)

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Helicobacter pylori cagA, iceA and vacA status in Taiwanese patients with peptic ulcer and gastritis. / Perng, Chin Lin; Lin, Hwai Jeng; Sun, I. Chen; Tseng, Guan Ying.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 18, No. 11, 2003, p. 1244-1249.

Research output: Contribution to journalArticle

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abstract = "Background: Helicobacter pylori causes chronic gastritis, peptic ulcer, gastric cancer and mucosa-associated lymphoid tissue (MALT) lymphoma. Different genotypes of H. pylori are confirmed from diverse geographical areas. Its association with clinical diseases remains controversial. The aim of the present study was to investigate the H. pylori vacuolating cytotoxin (vacA) alleles, cytotoxin-associated gene (cagA) and iceA, in patients with peptic ulcer and gastritis. Methods: We enrolled patients with peptic ulcer and chronic gastritis. Biopsy specimens were obtained from the antrum and lower body of the stomach. DNA extraction and polymerase chain reaction (PCR) were used to detect the presence or absence of cagA and to assess the polymorphism of vacA and iceA. Results: A total of 133 patients (57 gastric ulcer, 52 duodenal ulcer, 24 chronic gastritis) had positive PCR results from biopsy specimens. Concerning genotypes, we found cagA (79{\%} in the antrum, 92{\%} in the body) and iceA1 (73{\%} in the antrum, 82.8{\%} in the body) strains in the majority of patients. The dominant vacA subtype was s1a (74.4{\%} in the antrum, 75{\%} in the body), followed by s1c (51.1{\%} in the antrum, 60.5{\%} in the body). In the middle region, the m2 strain dominated (49.6{\%} in the antrum, 41.4{\%} in the body), followed by m1T (19.5{\%} in the antrum, 9.5{\%} in the body). Mixed infection occurred in 89 patients (67{\%}).There was no statistical difference in genotypes among the three groups. Conclusion: In Taiwan, H. pylori with positive cagA and iceA1 was found in the majority of cases. H. pylori with vacA s1a strains was the most common vacA subtype, followed by s1c, while s1b was rare. In the middle region, the m2 subtype was predominant followed by m1T. There was no significant association between genotypes and clinical diseases.",
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T1 - Helicobacter pylori cagA, iceA and vacA status in Taiwanese patients with peptic ulcer and gastritis

AU - Perng, Chin Lin

AU - Lin, Hwai Jeng

AU - Sun, I. Chen

AU - Tseng, Guan Ying

PY - 2003

Y1 - 2003

N2 - Background: Helicobacter pylori causes chronic gastritis, peptic ulcer, gastric cancer and mucosa-associated lymphoid tissue (MALT) lymphoma. Different genotypes of H. pylori are confirmed from diverse geographical areas. Its association with clinical diseases remains controversial. The aim of the present study was to investigate the H. pylori vacuolating cytotoxin (vacA) alleles, cytotoxin-associated gene (cagA) and iceA, in patients with peptic ulcer and gastritis. Methods: We enrolled patients with peptic ulcer and chronic gastritis. Biopsy specimens were obtained from the antrum and lower body of the stomach. DNA extraction and polymerase chain reaction (PCR) were used to detect the presence or absence of cagA and to assess the polymorphism of vacA and iceA. Results: A total of 133 patients (57 gastric ulcer, 52 duodenal ulcer, 24 chronic gastritis) had positive PCR results from biopsy specimens. Concerning genotypes, we found cagA (79% in the antrum, 92% in the body) and iceA1 (73% in the antrum, 82.8% in the body) strains in the majority of patients. The dominant vacA subtype was s1a (74.4% in the antrum, 75% in the body), followed by s1c (51.1% in the antrum, 60.5% in the body). In the middle region, the m2 strain dominated (49.6% in the antrum, 41.4% in the body), followed by m1T (19.5% in the antrum, 9.5% in the body). Mixed infection occurred in 89 patients (67%).There was no statistical difference in genotypes among the three groups. Conclusion: In Taiwan, H. pylori with positive cagA and iceA1 was found in the majority of cases. H. pylori with vacA s1a strains was the most common vacA subtype, followed by s1c, while s1b was rare. In the middle region, the m2 subtype was predominant followed by m1T. There was no significant association between genotypes and clinical diseases.

AB - Background: Helicobacter pylori causes chronic gastritis, peptic ulcer, gastric cancer and mucosa-associated lymphoid tissue (MALT) lymphoma. Different genotypes of H. pylori are confirmed from diverse geographical areas. Its association with clinical diseases remains controversial. The aim of the present study was to investigate the H. pylori vacuolating cytotoxin (vacA) alleles, cytotoxin-associated gene (cagA) and iceA, in patients with peptic ulcer and gastritis. Methods: We enrolled patients with peptic ulcer and chronic gastritis. Biopsy specimens were obtained from the antrum and lower body of the stomach. DNA extraction and polymerase chain reaction (PCR) were used to detect the presence or absence of cagA and to assess the polymorphism of vacA and iceA. Results: A total of 133 patients (57 gastric ulcer, 52 duodenal ulcer, 24 chronic gastritis) had positive PCR results from biopsy specimens. Concerning genotypes, we found cagA (79% in the antrum, 92% in the body) and iceA1 (73% in the antrum, 82.8% in the body) strains in the majority of patients. The dominant vacA subtype was s1a (74.4% in the antrum, 75% in the body), followed by s1c (51.1% in the antrum, 60.5% in the body). In the middle region, the m2 strain dominated (49.6% in the antrum, 41.4% in the body), followed by m1T (19.5% in the antrum, 9.5% in the body). Mixed infection occurred in 89 patients (67%).There was no statistical difference in genotypes among the three groups. Conclusion: In Taiwan, H. pylori with positive cagA and iceA1 was found in the majority of cases. H. pylori with vacA s1a strains was the most common vacA subtype, followed by s1c, while s1b was rare. In the middle region, the m2 subtype was predominant followed by m1T. There was no significant association between genotypes and clinical diseases.

KW - Cytotoxin-associated gene (cagA)

KW - Genotypes

KW - Helicobacter pylori

KW - iceA

KW - Vacuolating cytotoxin (vacA)

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