Heat shock protein 90 stabilizes nucleolin to increase mRNA stability in mitosis

Shao An Wang, Hao Yi Li, Tsung I. Hsu, Shu Hui Chen, Chin Jen Wu, Wen Chang Chang, Jan Jong Hung

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Most studies on heat shock protein 90 (Hsp90) have focused on the involvement of Hsp90 in the interphase, whereas the role of this protein in the nucleus during mitosis remains largely unclear. In this study, we found that the level of the acetylated form of Hsp90 decreased dramatically during mitosis, which indicates more chaperone activity during mitosis. We thus probed proteins that interacted with Hsp90 by liquid chromatography/mass spectrometry (LC/MS) and found that nucleolin was one of those interacting proteins during mitosis. The nucleolin level decreased upon geldanamycin treatment, and Hsp90 maintained the cyclin-dependent kinase 1 (CDK1) activity to phosphorylate nucleolin at Thr-641/707. Mutation of Thr-641/707 resulted in the destabilization of nucleolin in mitosis. We globally screened the level of mitotic mRNAs and found that 229 mRNAs decreased during mitosis in the presence of geldanamycin. Furthermore, a bioinformatics tool and an RNA immunoprecipitation assay found that 16 mRNAs, including cadherin and Bcl-xl, were stabilized through the recruitment of nucleolin to the 3′-untranslated regions (3′-UTRs) of those genes. Overall, strong correlations exist between the up-regulation of Hsp90, nucleolin, and the mRNAs related to tumorigenesis of the lung. Our findings thus indicate that nucleolin stabilized by Hsp90 contributes to the lung tumorigenesis by increasing the level of many tumor-related mRNAs during mitosis.

Original languageEnglish
Pages (from-to)43816-43829
Number of pages14
JournalJournal of Biological Chemistry
Volume286
Issue number51
DOIs
Publication statusPublished - Dec 23 2011

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HSP90 Heat-Shock Proteins
RNA Stability
Mitosis
Messenger RNA
Carcinogenesis
CDC2 Protein Kinase
Lung
Proteins
Interphase
Liquid chromatography
3' Untranslated Regions
Cadherins
Bioinformatics
nucleolin
Computational Biology
Immunoprecipitation
Liquid Chromatography
Mass spectrometry
Tumors
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Heat shock protein 90 stabilizes nucleolin to increase mRNA stability in mitosis. / Wang, Shao An; Li, Hao Yi; Hsu, Tsung I.; Chen, Shu Hui; Wu, Chin Jen; Chang, Wen Chang; Hung, Jan Jong.

In: Journal of Biological Chemistry, Vol. 286, No. 51, 23.12.2011, p. 43816-43829.

Research output: Contribution to journalArticle

Wang, Shao An ; Li, Hao Yi ; Hsu, Tsung I. ; Chen, Shu Hui ; Wu, Chin Jen ; Chang, Wen Chang ; Hung, Jan Jong. / Heat shock protein 90 stabilizes nucleolin to increase mRNA stability in mitosis. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 51. pp. 43816-43829.
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