Heat Killed Lactobacillus reuteri GMNL-263 Reduces Fibrosis Effects on the Liver and Heart in High Fat Diet-Hamsters via TGF-β Suppression

Wei Jen Ting, Wei Wen Kuo, Dennis Jine Yuan Hsieh, Yu Lan Yeh, Cecilia Hsuan Day, Ya Hui Chen, Ray-Jade Chen, Viswanadha Vijaya Padma, Yi Hsing Chen, Chih Yang Huang

Research output: Contribution to journalArticle

15 Citations (Scopus)


Obesity is one of the major risk factors for nonalcoholic fatty liver disease (NAFLD), and NAFLD is highly associated with an increased risk of cardiovascular disease (CVD). Scholars have suggested that certain probiotics may significantly impact cardiovascular health, particularly certain Lactobacillus species, such as Lactobacillus reuteri GMNL-263 (Lr263) probiotics, which have been shown to reduce obesity and arteriosclerosis in vivo. In the present study, we examined the potential of heat-killed bacteria to attenuate high fat diet (HFD)-induced hepatic and cardiac damages and the possible underlying mechanism of the positive effects of heat-killed Lr263 oral supplements. Heat-killed Lr263 treatments (625 and 3125 mg/kg-hamster/day) were provided as a daily supplement by oral gavage to HFD-fed hamsters for eight weeks. The results show that heat-killed Lr263 treatments reduce fatty liver syndrome. Moreover, heat-killed Lactobacillus reuteri GMNL-263 supplementation in HFD hamsters also reduced fibrosis in the liver and heart by reducing transforming growth factor β (TGF-β) expression levels. In conclusion, heat-killed Lr263 can reduce lipid metabolic stress in HFD hamsters and decrease the risk of fatty liver and cardiovascular disease.

Original languageEnglish
Pages (from-to)25881-96
Number of pages16
JournalInternational Journal of Molecular Sciences
Issue number10
Publication statusPublished - 2015



  • Animals
  • Cricetinae
  • Diet, High-Fat
  • Fatty Liver
  • Fibrosis
  • Heart Diseases
  • Lactobacillus reuteri
  • Male
  • Obesity
  • Probiotics
  • Transforming Growth Factor beta
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this