Heat Killed Lactobacillus reuteri GMNL-263 Reduces Fibrosis Effects on the Liver and Heart in High Fat Diet-Hamsters via TGF-β Suppression

Wei Jen Ting, Wei Wen Kuo, Dennis Jine Yuan Hsieh, Yu Lan Yeh, Cecilia Hsuan Day, Ya Hui Chen, Ray-Jade Chen, Viswanadha Vijaya Padma, Yi Hsing Chen, Chih Yang Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Obesity is one of the major risk factors for nonalcoholic fatty liver disease (NAFLD), and NAFLD is highly associated with an increased risk of cardiovascular disease (CVD). Scholars have suggested that certain probiotics may significantly impact cardiovascular health, particularly certain Lactobacillus species, such as Lactobacillus reuteri GMNL-263 (Lr263) probiotics, which have been shown to reduce obesity and arteriosclerosis in vivo. In the present study, we examined the potential of heat-killed bacteria to attenuate high fat diet (HFD)-induced hepatic and cardiac damages and the possible underlying mechanism of the positive effects of heat-killed Lr263 oral supplements. Heat-killed Lr263 treatments (625 and 3125 mg/kg-hamster/day) were provided as a daily supplement by oral gavage to HFD-fed hamsters for eight weeks. The results show that heat-killed Lr263 treatments reduce fatty liver syndrome. Moreover, heat-killed Lactobacillus reuteri GMNL-263 supplementation in HFD hamsters also reduced fibrosis in the liver and heart by reducing transforming growth factor β (TGF-β) expression levels. In conclusion, heat-killed Lr263 can reduce lipid metabolic stress in HFD hamsters and decrease the risk of fatty liver and cardiovascular disease.

Original languageEnglish
Pages (from-to)25881-96
Number of pages16
JournalInternational Journal of Molecular Sciences
Volume16
Issue number10
DOIs
Publication statusPublished - 2015

Fingerprint

Lactobacillus reuteri
fibrosis
diets
hamsters
fats
Transforming Growth Factors
High Fat Diet
Nutrition
Oils and fats
liver
Cricetinae
Liver
Fibrosis
Hot Temperature
Fats
retarding
heat
obesity
Probiotics
supplements

Keywords

  • Animals
  • Cricetinae
  • Diet, High-Fat
  • Fatty Liver
  • Fibrosis
  • Heart Diseases
  • Lactobacillus reuteri
  • Male
  • Obesity
  • Probiotics
  • Transforming Growth Factor beta
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Heat Killed Lactobacillus reuteri GMNL-263 Reduces Fibrosis Effects on the Liver and Heart in High Fat Diet-Hamsters via TGF-β Suppression. / Ting, Wei Jen; Kuo, Wei Wen; Hsieh, Dennis Jine Yuan; Yeh, Yu Lan; Day, Cecilia Hsuan; Chen, Ya Hui; Chen, Ray-Jade; Padma, Viswanadha Vijaya; Chen, Yi Hsing; Huang, Chih Yang.

In: International Journal of Molecular Sciences, Vol. 16, No. 10, 2015, p. 25881-96.

Research output: Contribution to journalArticle

Ting, Wei Jen ; Kuo, Wei Wen ; Hsieh, Dennis Jine Yuan ; Yeh, Yu Lan ; Day, Cecilia Hsuan ; Chen, Ya Hui ; Chen, Ray-Jade ; Padma, Viswanadha Vijaya ; Chen, Yi Hsing ; Huang, Chih Yang. / Heat Killed Lactobacillus reuteri GMNL-263 Reduces Fibrosis Effects on the Liver and Heart in High Fat Diet-Hamsters via TGF-β Suppression. In: International Journal of Molecular Sciences. 2015 ; Vol. 16, No. 10. pp. 25881-96.
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AU - Kuo, Wei Wen

AU - Hsieh, Dennis Jine Yuan

AU - Yeh, Yu Lan

AU - Day, Cecilia Hsuan

AU - Chen, Ya Hui

AU - Chen, Ray-Jade

AU - Padma, Viswanadha Vijaya

AU - Chen, Yi Hsing

AU - Huang, Chih Yang

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AB - Obesity is one of the major risk factors for nonalcoholic fatty liver disease (NAFLD), and NAFLD is highly associated with an increased risk of cardiovascular disease (CVD). Scholars have suggested that certain probiotics may significantly impact cardiovascular health, particularly certain Lactobacillus species, such as Lactobacillus reuteri GMNL-263 (Lr263) probiotics, which have been shown to reduce obesity and arteriosclerosis in vivo. In the present study, we examined the potential of heat-killed bacteria to attenuate high fat diet (HFD)-induced hepatic and cardiac damages and the possible underlying mechanism of the positive effects of heat-killed Lr263 oral supplements. Heat-killed Lr263 treatments (625 and 3125 mg/kg-hamster/day) were provided as a daily supplement by oral gavage to HFD-fed hamsters for eight weeks. The results show that heat-killed Lr263 treatments reduce fatty liver syndrome. Moreover, heat-killed Lactobacillus reuteri GMNL-263 supplementation in HFD hamsters also reduced fibrosis in the liver and heart by reducing transforming growth factor β (TGF-β) expression levels. In conclusion, heat-killed Lr263 can reduce lipid metabolic stress in HFD hamsters and decrease the risk of fatty liver and cardiovascular disease.

KW - Animals

KW - Cricetinae

KW - Diet, High-Fat

KW - Fatty Liver

KW - Fibrosis

KW - Heart Diseases

KW - Lactobacillus reuteri

KW - Male

KW - Obesity

KW - Probiotics

KW - Transforming Growth Factor beta

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