Heart failure enhanced pulmonary vein arrhythmogenesis and dysregulated sodium and calcium homeostasis with increased calcium sparks

Shih Lin Chang, Yao Chang Chen, Yung Hsin Yeh, Yung Kuo Lin, Tsu Juey Wu, Cheng I. Lin, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Introduction: Late sodium currents and intracellular Ca2+ (Ca2+i) dynamics play an important role in arrhythmogenesis of pulmonary vein (PV) and heart failure (HF). It is not clear whether HF enhances PV arrhythmogenesis through modulation of Ca2+ homeostasis and increased late sodium currents. The aim of this study was to investigate the sodium and calcium homeostasis in PV cardiomyocytes with HF. Methods and Results: Whole-cell patch clamp was used to investigate the action potentials and ionic currents in isolated rabbit single PV cardiomyocytes with and without rapid pacing induced HF. The Ca2+i dynamics were evaluated through fluorescence and confocal microscopy. As compared to control PV cardiomyocytes (n = 18), HF PV cardiomyocytes (n = 13) had a higher incidence of delayed afterdepolarization (45% vs 13%, P <0.05) and faster spontaneous activity (3.0 ± 0.2 vs 2.1 ± 0.2 Hz, P <0.05). HF PV cardiomyocytes had increased late Na+ currents, Na +/Ca2+ exchanger currents, and transient inward currents, but had decreased Na+ currents or L-type calcium currents. HF PV cardiomyocytes with pacemaker activity had larger Ca2+i transients (R410/485, 0.18 ± 0.04 vs 0.11 ± 0.02, P <0.05), and sarcoplasmic reticulum Ca2+ stores. Moreover, HF PV cardiomyocytes with pacemaker activity (n = 18) had higher incidence (95% vs 70%, P <0.05), frequency (7.8 ± 3.1 vs 2.3 ± 1.2 spark/mm/s, P <0.05), amplitude (F/F0, 3.2 ± 0.8 vs 1.9 ± 0.5, P <0.05), and longer decay time (65 ± 3 vs 48 ± 4 ms, P <0.05) of Ca2+ sparks than control PV cardiomyocytes with pacemaker activity (n = 18). Conclusions: Dysregulated sodium and calcium homeostasis, and enhanced calcium sparks promote arrhythmogenesis of PV cardiomyocytes in HF, which may play an important role in the development of atrial fibrillation.

Original languageEnglish
Pages (from-to)1378-1386
Number of pages9
JournalJournal of Cardiovascular Electrophysiology
Volume22
Issue number12
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Calcium Signaling
Pulmonary Veins
Homeostasis
Cardiac Myocytes
Heart Failure
Sodium
Calcium
Incidence
Sarcoplasmic Reticulum
Fluorescence Microscopy
Confocal Microscopy
Atrial Fibrillation
Action Potentials
Rabbits

Keywords

  • atrial fibrillation
  • Ca sparks
  • calcium homeostasis
  • late sodium

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Heart failure enhanced pulmonary vein arrhythmogenesis and dysregulated sodium and calcium homeostasis with increased calcium sparks. / Chang, Shih Lin; Chen, Yao Chang; Yeh, Yung Hsin; Lin, Yung Kuo; Wu, Tsu Juey; Lin, Cheng I.; Chen, Shih Ann; Chen, Yi Jen.

In: Journal of Cardiovascular Electrophysiology, Vol. 22, No. 12, 12.2011, p. 1378-1386.

Research output: Contribution to journalArticle

Chang, Shih Lin ; Chen, Yao Chang ; Yeh, Yung Hsin ; Lin, Yung Kuo ; Wu, Tsu Juey ; Lin, Cheng I. ; Chen, Shih Ann ; Chen, Yi Jen. / Heart failure enhanced pulmonary vein arrhythmogenesis and dysregulated sodium and calcium homeostasis with increased calcium sparks. In: Journal of Cardiovascular Electrophysiology. 2011 ; Vol. 22, No. 12. pp. 1378-1386.
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T1 - Heart failure enhanced pulmonary vein arrhythmogenesis and dysregulated sodium and calcium homeostasis with increased calcium sparks

AU - Chang, Shih Lin

AU - Chen, Yao Chang

AU - Yeh, Yung Hsin

AU - Lin, Yung Kuo

AU - Wu, Tsu Juey

AU - Lin, Cheng I.

AU - Chen, Shih Ann

AU - Chen, Yi Jen

PY - 2011/12

Y1 - 2011/12

N2 - Introduction: Late sodium currents and intracellular Ca2+ (Ca2+i) dynamics play an important role in arrhythmogenesis of pulmonary vein (PV) and heart failure (HF). It is not clear whether HF enhances PV arrhythmogenesis through modulation of Ca2+ homeostasis and increased late sodium currents. The aim of this study was to investigate the sodium and calcium homeostasis in PV cardiomyocytes with HF. Methods and Results: Whole-cell patch clamp was used to investigate the action potentials and ionic currents in isolated rabbit single PV cardiomyocytes with and without rapid pacing induced HF. The Ca2+i dynamics were evaluated through fluorescence and confocal microscopy. As compared to control PV cardiomyocytes (n = 18), HF PV cardiomyocytes (n = 13) had a higher incidence of delayed afterdepolarization (45% vs 13%, P <0.05) and faster spontaneous activity (3.0 ± 0.2 vs 2.1 ± 0.2 Hz, P <0.05). HF PV cardiomyocytes had increased late Na+ currents, Na +/Ca2+ exchanger currents, and transient inward currents, but had decreased Na+ currents or L-type calcium currents. HF PV cardiomyocytes with pacemaker activity had larger Ca2+i transients (R410/485, 0.18 ± 0.04 vs 0.11 ± 0.02, P <0.05), and sarcoplasmic reticulum Ca2+ stores. Moreover, HF PV cardiomyocytes with pacemaker activity (n = 18) had higher incidence (95% vs 70%, P <0.05), frequency (7.8 ± 3.1 vs 2.3 ± 1.2 spark/mm/s, P <0.05), amplitude (F/F0, 3.2 ± 0.8 vs 1.9 ± 0.5, P <0.05), and longer decay time (65 ± 3 vs 48 ± 4 ms, P <0.05) of Ca2+ sparks than control PV cardiomyocytes with pacemaker activity (n = 18). Conclusions: Dysregulated sodium and calcium homeostasis, and enhanced calcium sparks promote arrhythmogenesis of PV cardiomyocytes in HF, which may play an important role in the development of atrial fibrillation.

AB - Introduction: Late sodium currents and intracellular Ca2+ (Ca2+i) dynamics play an important role in arrhythmogenesis of pulmonary vein (PV) and heart failure (HF). It is not clear whether HF enhances PV arrhythmogenesis through modulation of Ca2+ homeostasis and increased late sodium currents. The aim of this study was to investigate the sodium and calcium homeostasis in PV cardiomyocytes with HF. Methods and Results: Whole-cell patch clamp was used to investigate the action potentials and ionic currents in isolated rabbit single PV cardiomyocytes with and without rapid pacing induced HF. The Ca2+i dynamics were evaluated through fluorescence and confocal microscopy. As compared to control PV cardiomyocytes (n = 18), HF PV cardiomyocytes (n = 13) had a higher incidence of delayed afterdepolarization (45% vs 13%, P <0.05) and faster spontaneous activity (3.0 ± 0.2 vs 2.1 ± 0.2 Hz, P <0.05). HF PV cardiomyocytes had increased late Na+ currents, Na +/Ca2+ exchanger currents, and transient inward currents, but had decreased Na+ currents or L-type calcium currents. HF PV cardiomyocytes with pacemaker activity had larger Ca2+i transients (R410/485, 0.18 ± 0.04 vs 0.11 ± 0.02, P <0.05), and sarcoplasmic reticulum Ca2+ stores. Moreover, HF PV cardiomyocytes with pacemaker activity (n = 18) had higher incidence (95% vs 70%, P <0.05), frequency (7.8 ± 3.1 vs 2.3 ± 1.2 spark/mm/s, P <0.05), amplitude (F/F0, 3.2 ± 0.8 vs 1.9 ± 0.5, P <0.05), and longer decay time (65 ± 3 vs 48 ± 4 ms, P <0.05) of Ca2+ sparks than control PV cardiomyocytes with pacemaker activity (n = 18). Conclusions: Dysregulated sodium and calcium homeostasis, and enhanced calcium sparks promote arrhythmogenesis of PV cardiomyocytes in HF, which may play an important role in the development of atrial fibrillation.

KW - atrial fibrillation

KW - Ca sparks

KW - calcium homeostasis

KW - late sodium

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