HDAC1,2 Knock-Out and HDACi Induced Cell Apoptosis in Imatinib-Resistant K562 Cells

Shu Huey Chen, Jyh Ming Chow, Yao Yu Hsieh, Chun Yu Lin, Kai Wen Hsu, Wen Shyang Hsieh, Wei Ming Chi, Beished M. Shabangu, Chia Hwa Lee

Research output: Contribution to journalArticle

Abstract

Since imatinib (Glivec or Gleevec) has been used to target the BCR-ABL fusion protein, chronic myeloid leukemia (CML) has become a manageable chronic disease with long-term survival. However, 15%-20% of CML patients ultimately develop resistance to imatinib and then progress to an accelerated phase and eventually to a blast crisis, limiting treatment options and resulting in a poor survival rate. Thus, we investigated whether histone deacetylase inhibitors (HDACis) could be used as a potential anticancer therapy for imatinib-resistant CML (IR-CML) patients. By applying a noninvasive apoptosis detection sensor (NIADS), we found that panobinostat significantly enhanced cell apoptosis in K562 cells. A further investigation showed that panobinostat induced apoptosis in both K562 and imatinib-resistant K562 (IR-K562) cells mainly via H3 and H4 histone acetylation, whereas panobinostat targeted cancer stem cells (CSCs) in IR-K562 cells. Using CRISPR/Cas9 genomic editing, we found that HDAC1 and HDAC2 knockout cells significantly induced cell apoptosis, indicating that the regulation of HDAC1 and HDAC2 is extremely important in maintaining K562 cell survival. All information in this study indicates that regulating HDAC activity provides therapeutic benefits against CML and IR-CML in the clinic.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume20
Issue number9
DOIs
Publication statusPublished - May 8 2019

Fingerprint

Histone Deacetylase Inhibitors
K562 Cells
apoptosis
Cell death
inhibitors
leukemias
Apoptosis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
cells
Emitter coupled logic circuits
Acetylation
Stem cells
Histones
acetylation
editing
Fusion reactions
Clustered Regularly Interspaced Short Palindromic Repeats
stem cells
Cells
blasts

Keywords

  • CML
  • CRISPR/Cas9
  • histone deacetylase inhibitor
  • imatinib
  • imatinib-resistant

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

HDAC1,2 Knock-Out and HDACi Induced Cell Apoptosis in Imatinib-Resistant K562 Cells. / Chen, Shu Huey; Chow, Jyh Ming; Hsieh, Yao Yu; Lin, Chun Yu; Hsu, Kai Wen; Hsieh, Wen Shyang; Chi, Wei Ming; Shabangu, Beished M.; Lee, Chia Hwa.

In: International Journal of Molecular Sciences, Vol. 20, No. 9, 08.05.2019.

Research output: Contribution to journalArticle

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