Haloperidol Abrogates Matrix Metalloproteinase-9 Expression by Inhibition of NF-κB Activation in Stimulated Human Monocytic Cells

Yueh Lun Lee, Che Jen Hsiao, Fan Li Lin, Jing Shiun Jan, Yung Chen Chou, Yen Yu Lin, Chih Kuang Chen, Kwok Keung Lam, George Hsiao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Much evidence has indicated that matrix metalloproteinases (MMPs) participate in the progression of neuroinflammatory disorders. The present study was undertaken to investigate the inhibitory effect and mechanism of the antipsychotic haloperidol on MMP activation in the stimulated THP-1 monocytic cells. Haloperidol exerted a strong inhibition on tumor necrosis factor- (TNF-) α-induced MMP-9 gelatinolysis of THP-1 cells. A concentration-dependent inhibitory effect of haloperidol was observed in TNF-α-induced protein and mRNA expression of MMP-9. On the other hand, haloperidol slightly affected cell viability and tissue inhibition of metalloproteinase-1 levels. It significantly inhibited the degradation of inhibitor-κB-α (IκBα) in activated cells. Moreover, it suppressed activated nuclear factor-κB (NF-κB) detected by a mobility shift assay, NF-κB reporter gene, and chromatin immunoprecipitation analyses. Consistent with NF-κB inhibition, haloperidol exerted a strong inhibition of lipopolysaccharide- (LPS-) induced MMP-9 gelatinolysis but not of transforming growth factor-β1-induced MMP-2. In in vivo studies, administration of haloperidol significantly attenuated LPS-induced intracerebral MMP-9 activation of the brain homogenate and the in situ in C57BL/6 mice. In conclusion, the selective anti-MMP-9 activation of haloperidol could possibly involve the inhibition of the NF-κB signal pathway. Hence, it was found that haloperidol treatment may represent a bystander of anti-MMP actions for its conventional psychotherapy.

Original languageEnglish
Article number9541459
Number of pages1
JournalMediators of Inflammation
Volume2018
DOIs
Publication statusPublished - Jan 1 2018

Keywords

  • Animals
  • Cell Survival/drug effects
  • Chromatin Immunoprecipitation
  • Haloperidol/pharmacology
  • Humans
  • I-kappa B Proteins/metabolism
  • Lipopolysaccharides/pharmacology
  • Matrix Metalloproteinase 9/metabolism
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B/metabolism
  • Signal Transduction/drug effects

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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