Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids

Tsai Mu Cheng, Simon J T Mao, Shiau Ting Lai, Chia Ching Chang, Ming Chi Yang, Nai Chi Chen, Shiu Ching Chou, Ju Pin Pan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.

Original languageEnglish
Pages (from-to)303-316
Number of pages14
JournalFree Radical Research
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

Fingerprint

Oxidative stress
Unsaturated Fatty Acids
Peroxidase
Hemoglobins
Oxidative Stress
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Sodium Azide
Catalase
Hemoglobin H
Probucol
Human Umbilical Vein Endothelial Cells
Oxidation
Linoleic Acid
Membrane Lipids
Endothelial cells
Cell membranes
Lipid Peroxidation
Reactive Oxygen Species
Antioxidants

Keywords

  • cell adhesion
  • Hemoglobin
  • ICAM-1
  • LDL peroxidation
  • peroxidase
  • probucol
  • VCAM-1

ASJC Scopus subject areas

  • Biochemistry

Cite this

Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids. / Cheng, Tsai Mu; Mao, Simon J T; Lai, Shiau Ting; Chang, Chia Ching; Yang, Ming Chi; Chen, Nai Chi; Chou, Shiu Ching; Pan, Ju Pin.

In: Free Radical Research, Vol. 45, No. 3, 03.2011, p. 303-316.

Research output: Contribution to journalArticle

Cheng, Tsai Mu ; Mao, Simon J T ; Lai, Shiau Ting ; Chang, Chia Ching ; Yang, Ming Chi ; Chen, Nai Chi ; Chou, Shiu Ching ; Pan, Ju Pin. / Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids. In: Free Radical Research. 2011 ; Vol. 45, No. 3. pp. 303-316.
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