Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids

Tsai Mu Cheng, Simon J T Mao, Shiau Ting Lai, Chia Ching Chang, Ming Chi Yang, Nai Chi Chen, Shiu Ching Chou, Ju Pin Pan

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6 Citations (Scopus)

Abstract

Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.

Original languageEnglish
Pages (from-to)303-316
Number of pages14
JournalFree Radical Research
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

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Keywords

  • cell adhesion
  • Hemoglobin
  • ICAM-1
  • LDL peroxidation
  • peroxidase
  • probucol
  • VCAM-1

ASJC Scopus subject areas

  • Biochemistry

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