GSTM1, GSTT1, GSTP1, and GSTA1 genetic variants are not associated with coronary artery disease in Taiwan

Hseng Long Yeh, Li Tang Kuo, Fung Chang Sung, Cheng-Wen Chiang, Chih Ching Yeh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background and objective: The genetic variants of xenobiotic-metabolizing enzymes, such as those encoded by glutathione-S-transferase (GST) genes, may be associated with the risk of coronary artery disease (CAD). To investigate the genetic factors for CAD, we examined the GSTM1, GSTT1, GSTP1, and GSTA1 genotypes in a CAD cohort in Taiwan. Methods: Our study included 458 CAD participants and 209 control participants who received coronary angiography to assess CAD. The severity of CAD was defined as the number of coronary vessels with 50% or greater stenosis. Sequence variation of the GSTM1 and GSTT1 genes was determined using a polymerase chain reaction (PCR). The GSTP1 (Ile105Val), and GSTA1 (-69C. >. T) genetic variants were identified using a combination of PCR and restriction fragment length polymorphism analysis. Logistic regression analysis was used to calculate the odds ratios (ORs) and 95% confidence intervals. Results: Among the GST genetic variants examined, the GSTT1 null genotype was more prevalent in CAD participants with 3 stenosed vessels than in control participants (OR. =. 1.64, P=. .02). This association was no longer observed after adjusting for age, sex, smoking, alcohol use, diabetes mellitus, and serum levels of total cholesterol and high-density lipoprotein cholesterol (OR. =. 1.28, P=. .40). Both univariate and multivariate logistic regression analyses found no significant associations between CAD and the other genetic variants, either separately or in combination. In addition, no effects of interactions between the genotypes and environmental factors, such as cigarette smoking, were significantly associated with the risk of CAD. Conclusion: The GST genetic variants examined were not associated with susceptibility to CAD in our Taiwanese cohort. This null association requires further confirmation with larger samples.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalGene
Volume523
Issue number1
DOIs
Publication statusPublished - Jul 1 2013

Fingerprint

Taiwan
Coronary Artery Disease
Glutathione Transferase
Odds Ratio
Genotype
Logistic Models
Smoking
Regression Analysis
Polymerase Chain Reaction
Xenobiotics
Coronary Angiography
Restriction Fragment Length Polymorphisms
HDL Cholesterol
Genes
Coronary Vessels
Diabetes Mellitus
Pathologic Constriction
Cholesterol
Alcohols
Confidence Intervals

Keywords

  • Coronary artery disease
  • Glutathione-S-transferase
  • Polymorphisms

ASJC Scopus subject areas

  • Genetics

Cite this

GSTM1, GSTT1, GSTP1, and GSTA1 genetic variants are not associated with coronary artery disease in Taiwan. / Yeh, Hseng Long; Kuo, Li Tang; Sung, Fung Chang; Chiang, Cheng-Wen; Yeh, Chih Ching.

In: Gene, Vol. 523, No. 1, 01.07.2013, p. 64-69.

Research output: Contribution to journalArticle

Yeh, Hseng Long ; Kuo, Li Tang ; Sung, Fung Chang ; Chiang, Cheng-Wen ; Yeh, Chih Ching. / GSTM1, GSTT1, GSTP1, and GSTA1 genetic variants are not associated with coronary artery disease in Taiwan. In: Gene. 2013 ; Vol. 523, No. 1. pp. 64-69.
@article{6b4ffe75930f4bd280d93f9623fa2eee,
title = "GSTM1, GSTT1, GSTP1, and GSTA1 genetic variants are not associated with coronary artery disease in Taiwan",
abstract = "Background and objective: The genetic variants of xenobiotic-metabolizing enzymes, such as those encoded by glutathione-S-transferase (GST) genes, may be associated with the risk of coronary artery disease (CAD). To investigate the genetic factors for CAD, we examined the GSTM1, GSTT1, GSTP1, and GSTA1 genotypes in a CAD cohort in Taiwan. Methods: Our study included 458 CAD participants and 209 control participants who received coronary angiography to assess CAD. The severity of CAD was defined as the number of coronary vessels with 50{\%} or greater stenosis. Sequence variation of the GSTM1 and GSTT1 genes was determined using a polymerase chain reaction (PCR). The GSTP1 (Ile105Val), and GSTA1 (-69C. >. T) genetic variants were identified using a combination of PCR and restriction fragment length polymorphism analysis. Logistic regression analysis was used to calculate the odds ratios (ORs) and 95{\%} confidence intervals. Results: Among the GST genetic variants examined, the GSTT1 null genotype was more prevalent in CAD participants with 3 stenosed vessels than in control participants (OR. =. 1.64, P=. .02). This association was no longer observed after adjusting for age, sex, smoking, alcohol use, diabetes mellitus, and serum levels of total cholesterol and high-density lipoprotein cholesterol (OR. =. 1.28, P=. .40). Both univariate and multivariate logistic regression analyses found no significant associations between CAD and the other genetic variants, either separately or in combination. In addition, no effects of interactions between the genotypes and environmental factors, such as cigarette smoking, were significantly associated with the risk of CAD. Conclusion: The GST genetic variants examined were not associated with susceptibility to CAD in our Taiwanese cohort. This null association requires further confirmation with larger samples.",
keywords = "Coronary artery disease, Glutathione-S-transferase, Polymorphisms",
author = "Yeh, {Hseng Long} and Kuo, {Li Tang} and Sung, {Fung Chang} and Cheng-Wen Chiang and Yeh, {Chih Ching}",
year = "2013",
month = "7",
day = "1",
doi = "10.1016/j.gene.2013.02.052",
language = "English",
volume = "523",
pages = "64--69",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - GSTM1, GSTT1, GSTP1, and GSTA1 genetic variants are not associated with coronary artery disease in Taiwan

AU - Yeh, Hseng Long

AU - Kuo, Li Tang

AU - Sung, Fung Chang

AU - Chiang, Cheng-Wen

AU - Yeh, Chih Ching

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Background and objective: The genetic variants of xenobiotic-metabolizing enzymes, such as those encoded by glutathione-S-transferase (GST) genes, may be associated with the risk of coronary artery disease (CAD). To investigate the genetic factors for CAD, we examined the GSTM1, GSTT1, GSTP1, and GSTA1 genotypes in a CAD cohort in Taiwan. Methods: Our study included 458 CAD participants and 209 control participants who received coronary angiography to assess CAD. The severity of CAD was defined as the number of coronary vessels with 50% or greater stenosis. Sequence variation of the GSTM1 and GSTT1 genes was determined using a polymerase chain reaction (PCR). The GSTP1 (Ile105Val), and GSTA1 (-69C. >. T) genetic variants were identified using a combination of PCR and restriction fragment length polymorphism analysis. Logistic regression analysis was used to calculate the odds ratios (ORs) and 95% confidence intervals. Results: Among the GST genetic variants examined, the GSTT1 null genotype was more prevalent in CAD participants with 3 stenosed vessels than in control participants (OR. =. 1.64, P=. .02). This association was no longer observed after adjusting for age, sex, smoking, alcohol use, diabetes mellitus, and serum levels of total cholesterol and high-density lipoprotein cholesterol (OR. =. 1.28, P=. .40). Both univariate and multivariate logistic regression analyses found no significant associations between CAD and the other genetic variants, either separately or in combination. In addition, no effects of interactions between the genotypes and environmental factors, such as cigarette smoking, were significantly associated with the risk of CAD. Conclusion: The GST genetic variants examined were not associated with susceptibility to CAD in our Taiwanese cohort. This null association requires further confirmation with larger samples.

AB - Background and objective: The genetic variants of xenobiotic-metabolizing enzymes, such as those encoded by glutathione-S-transferase (GST) genes, may be associated with the risk of coronary artery disease (CAD). To investigate the genetic factors for CAD, we examined the GSTM1, GSTT1, GSTP1, and GSTA1 genotypes in a CAD cohort in Taiwan. Methods: Our study included 458 CAD participants and 209 control participants who received coronary angiography to assess CAD. The severity of CAD was defined as the number of coronary vessels with 50% or greater stenosis. Sequence variation of the GSTM1 and GSTT1 genes was determined using a polymerase chain reaction (PCR). The GSTP1 (Ile105Val), and GSTA1 (-69C. >. T) genetic variants were identified using a combination of PCR and restriction fragment length polymorphism analysis. Logistic regression analysis was used to calculate the odds ratios (ORs) and 95% confidence intervals. Results: Among the GST genetic variants examined, the GSTT1 null genotype was more prevalent in CAD participants with 3 stenosed vessels than in control participants (OR. =. 1.64, P=. .02). This association was no longer observed after adjusting for age, sex, smoking, alcohol use, diabetes mellitus, and serum levels of total cholesterol and high-density lipoprotein cholesterol (OR. =. 1.28, P=. .40). Both univariate and multivariate logistic regression analyses found no significant associations between CAD and the other genetic variants, either separately or in combination. In addition, no effects of interactions between the genotypes and environmental factors, such as cigarette smoking, were significantly associated with the risk of CAD. Conclusion: The GST genetic variants examined were not associated with susceptibility to CAD in our Taiwanese cohort. This null association requires further confirmation with larger samples.

KW - Coronary artery disease

KW - Glutathione-S-transferase

KW - Polymorphisms

UR - http://www.scopus.com/inward/record.url?scp=84877804176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877804176&partnerID=8YFLogxK

U2 - 10.1016/j.gene.2013.02.052

DO - 10.1016/j.gene.2013.02.052

M3 - Article

C2 - 23570881

AN - SCOPUS:84877804176

VL - 523

SP - 64

EP - 69

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -