Growth-inhibitory effects of the red alga Gelidium amansii on cultured cells

Yue Hwa Chen, Ching Jung Tu, Hsiao Ting Wu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The objective of this study was to investigate the effects of Gelidium amansii, an edible red agar cultivated off the northeast coast of Taiwan, on the growth of two lines of cancer cells, murine hepatoma (Hepa-1) and human leukemia (HL-60) cells, as well as a normal cell line, murine embryo fibroblast cells (NIH-3T3). The potential role of G. amansii on the induction of apoptosis was also examined. The results indicated that all extracts from G. amansii, including phosphate-buffered saline (PBS) and methanol extracts from dried algae as well as the dimethyl sulfoxide (DMSO) extract from freeze-dried G. amansii agar, inhibited the growth of Hepa-1 and NIH-3T3 cells, but not the growth of HL-60 cells. Annexin V-positive cells were observed in methanol and DMSO extract-treated, but not PBS extract-treated Hepa-1 and NIH-3T3 cells, suggesting that the lipid-soluble extracts of G. amansii induced apoptosis. In summary, extracts of G. amansii from various preparations exhibited antiproliferative effects on Hepa-1 and NIH-3T3 cells, and apoptosis may play a role in the methanol and DMSO extract-induced inhibitory effects. However, the antiproliferative effects of PBS extracts was not through apoptosis. Moreover, the growth-inhibitory effects of G. amansii were not specific to cancer cells.

Original languageEnglish
Pages (from-to)180-184
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number2
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Rhodophyta
NIH 3T3 Cells
Cultured Cells
Dimethyl Sulfoxide
Apoptosis
Methanol
HL-60 Cells
Phosphates
Growth
Agar
Annexin A5
Taiwan
Hepatocellular Carcinoma
Neoplasms
Leukemia
Embryonic Structures
Fibroblasts
Lipids
Cell Line

Keywords

  • Annexin V staining
  • Apoptosis
  • Gelidium amansii
  • Growth inhibition

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Growth-inhibitory effects of the red alga Gelidium amansii on cultured cells. / Chen, Yue Hwa; Tu, Ching Jung; Wu, Hsiao Ting.

In: Biological and Pharmaceutical Bulletin, Vol. 27, No. 2, 02.2004, p. 180-184.

Research output: Contribution to journalArticle

@article{dd99f155df77418ab84f72b8d008ee3b,
title = "Growth-inhibitory effects of the red alga Gelidium amansii on cultured cells",
abstract = "The objective of this study was to investigate the effects of Gelidium amansii, an edible red agar cultivated off the northeast coast of Taiwan, on the growth of two lines of cancer cells, murine hepatoma (Hepa-1) and human leukemia (HL-60) cells, as well as a normal cell line, murine embryo fibroblast cells (NIH-3T3). The potential role of G. amansii on the induction of apoptosis was also examined. The results indicated that all extracts from G. amansii, including phosphate-buffered saline (PBS) and methanol extracts from dried algae as well as the dimethyl sulfoxide (DMSO) extract from freeze-dried G. amansii agar, inhibited the growth of Hepa-1 and NIH-3T3 cells, but not the growth of HL-60 cells. Annexin V-positive cells were observed in methanol and DMSO extract-treated, but not PBS extract-treated Hepa-1 and NIH-3T3 cells, suggesting that the lipid-soluble extracts of G. amansii induced apoptosis. In summary, extracts of G. amansii from various preparations exhibited antiproliferative effects on Hepa-1 and NIH-3T3 cells, and apoptosis may play a role in the methanol and DMSO extract-induced inhibitory effects. However, the antiproliferative effects of PBS extracts was not through apoptosis. Moreover, the growth-inhibitory effects of G. amansii were not specific to cancer cells.",
keywords = "Annexin V staining, Apoptosis, Gelidium amansii, Growth inhibition",
author = "Chen, {Yue Hwa} and Tu, {Ching Jung} and Wu, {Hsiao Ting}",
year = "2004",
month = "2",
doi = "10.1248/bpb.27.180",
language = "English",
volume = "27",
pages = "180--184",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "2",

}

TY - JOUR

T1 - Growth-inhibitory effects of the red alga Gelidium amansii on cultured cells

AU - Chen, Yue Hwa

AU - Tu, Ching Jung

AU - Wu, Hsiao Ting

PY - 2004/2

Y1 - 2004/2

N2 - The objective of this study was to investigate the effects of Gelidium amansii, an edible red agar cultivated off the northeast coast of Taiwan, on the growth of two lines of cancer cells, murine hepatoma (Hepa-1) and human leukemia (HL-60) cells, as well as a normal cell line, murine embryo fibroblast cells (NIH-3T3). The potential role of G. amansii on the induction of apoptosis was also examined. The results indicated that all extracts from G. amansii, including phosphate-buffered saline (PBS) and methanol extracts from dried algae as well as the dimethyl sulfoxide (DMSO) extract from freeze-dried G. amansii agar, inhibited the growth of Hepa-1 and NIH-3T3 cells, but not the growth of HL-60 cells. Annexin V-positive cells were observed in methanol and DMSO extract-treated, but not PBS extract-treated Hepa-1 and NIH-3T3 cells, suggesting that the lipid-soluble extracts of G. amansii induced apoptosis. In summary, extracts of G. amansii from various preparations exhibited antiproliferative effects on Hepa-1 and NIH-3T3 cells, and apoptosis may play a role in the methanol and DMSO extract-induced inhibitory effects. However, the antiproliferative effects of PBS extracts was not through apoptosis. Moreover, the growth-inhibitory effects of G. amansii were not specific to cancer cells.

AB - The objective of this study was to investigate the effects of Gelidium amansii, an edible red agar cultivated off the northeast coast of Taiwan, on the growth of two lines of cancer cells, murine hepatoma (Hepa-1) and human leukemia (HL-60) cells, as well as a normal cell line, murine embryo fibroblast cells (NIH-3T3). The potential role of G. amansii on the induction of apoptosis was also examined. The results indicated that all extracts from G. amansii, including phosphate-buffered saline (PBS) and methanol extracts from dried algae as well as the dimethyl sulfoxide (DMSO) extract from freeze-dried G. amansii agar, inhibited the growth of Hepa-1 and NIH-3T3 cells, but not the growth of HL-60 cells. Annexin V-positive cells were observed in methanol and DMSO extract-treated, but not PBS extract-treated Hepa-1 and NIH-3T3 cells, suggesting that the lipid-soluble extracts of G. amansii induced apoptosis. In summary, extracts of G. amansii from various preparations exhibited antiproliferative effects on Hepa-1 and NIH-3T3 cells, and apoptosis may play a role in the methanol and DMSO extract-induced inhibitory effects. However, the antiproliferative effects of PBS extracts was not through apoptosis. Moreover, the growth-inhibitory effects of G. amansii were not specific to cancer cells.

KW - Annexin V staining

KW - Apoptosis

KW - Gelidium amansii

KW - Growth inhibition

UR - http://www.scopus.com/inward/record.url?scp=16544365728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544365728&partnerID=8YFLogxK

U2 - 10.1248/bpb.27.180

DO - 10.1248/bpb.27.180

M3 - Article

C2 - 14758029

AN - SCOPUS:16544365728

VL - 27

SP - 180

EP - 184

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 2

ER -