Growth inhibition of cultured smooth muscle cells by corrosion products of 316 L stainless steel wire

Chun Che Shih, Chun Ming Shih, Yuh Lien Chen, Yea Yang Su, Jeng Shong Shih, Ching Fai Kwok, Shing Jong Lin

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The potential cytotoxicity on vascular smooth muscle cells of corrosion products from 316 L stainless steel, one of most popular biomaterials of intravascular stents, has not been highlighted. In this investigation, 316 L stainless steel wires were corroded in Dulbecco's modified eagle's medium with applied constant electrochemical breakdown voltage, and the supernatant and precipitates of corrosion products were prepared as culture media. The effects of different concentrations of corrosion products on the growth of rat aortic smooth muscle cells were conducted with the [ 3H]-thymidine uptake test and cell cycle sorter. Both the supernatant and precipitates of corrosion products were toxic to the primary culture of smooth muscle cells. The growth inhibition was correlated well with the increased nickel ions in the corrosion products when nickel concentration was above 11.7 ppm. The corrosion products also changed cell morphology and induced cell necrosis. The cell growth inhibition occurred at the G0/G1 to S transition phase. Similar to our recent study of nitinol stent wire, the present investigation also demonstrated the cytotoxicity of corrosion products of 316 L stainless steel stent wire on smooth muscle cells, which might affect the poststenting vascular response.

Original languageEnglish
Pages (from-to)200-207
Number of pages8
JournalJournal of Biomedical Materials Research
Volume57
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Stainless Steel
Muscle
Stainless steel
Cells
Wire
Corrosion
Stents
Cytotoxicity
Nickel
Precipitates
Poisons
Cell growth
Biocompatible Materials
Electric breakdown
Cell culture
Biomaterials
Thymidine
Culture Media
Rats
Ions

Keywords

  • 316 L stainless steel
  • Corrosion
  • Cytotoxicity
  • Nickel
  • Smooth muscle cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Growth inhibition of cultured smooth muscle cells by corrosion products of 316 L stainless steel wire. / Shih, Chun Che; Shih, Chun Ming; Chen, Yuh Lien; Su, Yea Yang; Shih, Jeng Shong; Kwok, Ching Fai; Lin, Shing Jong.

In: Journal of Biomedical Materials Research, Vol. 57, No. 2, 2001, p. 200-207.

Research output: Contribution to journalArticle

Shih, Chun Che ; Shih, Chun Ming ; Chen, Yuh Lien ; Su, Yea Yang ; Shih, Jeng Shong ; Kwok, Ching Fai ; Lin, Shing Jong. / Growth inhibition of cultured smooth muscle cells by corrosion products of 316 L stainless steel wire. In: Journal of Biomedical Materials Research. 2001 ; Vol. 57, No. 2. pp. 200-207.
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