Growth factors-loaded calcium phosphate/polymer hybrid coating with sequential release behavior prepared via electrochemical deposition method

Jenny I Chun Sar, Yen Zhu Lin, Chung Kwei Lin, Tse Ying Liu, San Yuan Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, the dicalcium phosphate dehydrate (DCPD)/carboxymethyl hexanoyl chitosan (CHC) hybrid coating layer with sequential release of BMP-2 and TGF-β1 was deposited onto Ti6Al4V substrate by electrochemical co-precipitation method. Different concentrations of amphiphatic chitosan derivative (carboxymethyl hexanoyl chitosan, CHC), BMP-2 and TGF-β1-loaded PLA microspheres (PLA/TGF-β1 MSs) were co-precipitated with the precursor of DCPD. The resulting DCPD/CHC hybrid coating incorporated with BMP-2 and PLA/TGF-β1 MSs was designed for improving the osteoconductive and osteoinductive functionalities of Ti6Al4V. FTIR spectroscopy and X-ray diffraction were used to characterize these hybrid coatings. The drug release profile, MC3T3-E1 cell proliferation and alkaline phosphatase activity were used to investigate the functionality of these hybrid coatings. It was found that the incorporation of CHC could be used as a mean to manipulate the release behavior of growth factor from the DCPD/CHC hybrid coating. In addition, the BMP-2- and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited a sequential release of BMP-2 and TGF-β1 during the first and second two weeks, respectively. Importantly, the DCPD/CHC hybrid coating immersed in simulated body fluid gradually transformed to hydroxyapatite phase. Hence, the BMP-2 and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited the best cell viability and the highest ALP activity after 14. days. The biocompatible hybrid coating with sequential release functionality proposed in the present study can enhance the osseointegration process and possess promising potential for clinical applications in bone implants and tissue engineering.

Original languageEnglish
JournalSurface and Coatings Technology
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

calcium phosphates
Calcium phosphate
Chitosan
Intercellular Signaling Peptides and Proteins
Polymers
coatings
phosphates
Phosphates
Coatings
polymers
calcium phosphate
phosphatases
body fluids
carboxymethyl-hexanoyl chitosan
tissue engineering
Body fluids
Bioelectric potentials
Phosphatases
Cell proliferation
Durapatite

Keywords

  • BMP-2
  • CHC
  • DCPD
  • Electrodeposition
  • TGF-β1

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Surfaces and Interfaces

Cite this

Growth factors-loaded calcium phosphate/polymer hybrid coating with sequential release behavior prepared via electrochemical deposition method. / Sar, Jenny I Chun; Lin, Yen Zhu; Lin, Chung Kwei; Liu, Tse Ying; Chen, San Yuan.

In: Surface and Coatings Technology, 2016.

Research output: Contribution to journalArticle

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abstract = "In this study, the dicalcium phosphate dehydrate (DCPD)/carboxymethyl hexanoyl chitosan (CHC) hybrid coating layer with sequential release of BMP-2 and TGF-β1 was deposited onto Ti6Al4V substrate by electrochemical co-precipitation method. Different concentrations of amphiphatic chitosan derivative (carboxymethyl hexanoyl chitosan, CHC), BMP-2 and TGF-β1-loaded PLA microspheres (PLA/TGF-β1 MSs) were co-precipitated with the precursor of DCPD. The resulting DCPD/CHC hybrid coating incorporated with BMP-2 and PLA/TGF-β1 MSs was designed for improving the osteoconductive and osteoinductive functionalities of Ti6Al4V. FTIR spectroscopy and X-ray diffraction were used to characterize these hybrid coatings. The drug release profile, MC3T3-E1 cell proliferation and alkaline phosphatase activity were used to investigate the functionality of these hybrid coatings. It was found that the incorporation of CHC could be used as a mean to manipulate the release behavior of growth factor from the DCPD/CHC hybrid coating. In addition, the BMP-2- and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited a sequential release of BMP-2 and TGF-β1 during the first and second two weeks, respectively. Importantly, the DCPD/CHC hybrid coating immersed in simulated body fluid gradually transformed to hydroxyapatite phase. Hence, the BMP-2 and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited the best cell viability and the highest ALP activity after 14. days. The biocompatible hybrid coating with sequential release functionality proposed in the present study can enhance the osseointegration process and possess promising potential for clinical applications in bone implants and tissue engineering.",
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AU - Sar, Jenny I Chun

AU - Lin, Yen Zhu

AU - Lin, Chung Kwei

AU - Liu, Tse Ying

AU - Chen, San Yuan

PY - 2016

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N2 - In this study, the dicalcium phosphate dehydrate (DCPD)/carboxymethyl hexanoyl chitosan (CHC) hybrid coating layer with sequential release of BMP-2 and TGF-β1 was deposited onto Ti6Al4V substrate by electrochemical co-precipitation method. Different concentrations of amphiphatic chitosan derivative (carboxymethyl hexanoyl chitosan, CHC), BMP-2 and TGF-β1-loaded PLA microspheres (PLA/TGF-β1 MSs) were co-precipitated with the precursor of DCPD. The resulting DCPD/CHC hybrid coating incorporated with BMP-2 and PLA/TGF-β1 MSs was designed for improving the osteoconductive and osteoinductive functionalities of Ti6Al4V. FTIR spectroscopy and X-ray diffraction were used to characterize these hybrid coatings. The drug release profile, MC3T3-E1 cell proliferation and alkaline phosphatase activity were used to investigate the functionality of these hybrid coatings. It was found that the incorporation of CHC could be used as a mean to manipulate the release behavior of growth factor from the DCPD/CHC hybrid coating. In addition, the BMP-2- and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited a sequential release of BMP-2 and TGF-β1 during the first and second two weeks, respectively. Importantly, the DCPD/CHC hybrid coating immersed in simulated body fluid gradually transformed to hydroxyapatite phase. Hence, the BMP-2 and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited the best cell viability and the highest ALP activity after 14. days. The biocompatible hybrid coating with sequential release functionality proposed in the present study can enhance the osseointegration process and possess promising potential for clinical applications in bone implants and tissue engineering.

AB - In this study, the dicalcium phosphate dehydrate (DCPD)/carboxymethyl hexanoyl chitosan (CHC) hybrid coating layer with sequential release of BMP-2 and TGF-β1 was deposited onto Ti6Al4V substrate by electrochemical co-precipitation method. Different concentrations of amphiphatic chitosan derivative (carboxymethyl hexanoyl chitosan, CHC), BMP-2 and TGF-β1-loaded PLA microspheres (PLA/TGF-β1 MSs) were co-precipitated with the precursor of DCPD. The resulting DCPD/CHC hybrid coating incorporated with BMP-2 and PLA/TGF-β1 MSs was designed for improving the osteoconductive and osteoinductive functionalities of Ti6Al4V. FTIR spectroscopy and X-ray diffraction were used to characterize these hybrid coatings. The drug release profile, MC3T3-E1 cell proliferation and alkaline phosphatase activity were used to investigate the functionality of these hybrid coatings. It was found that the incorporation of CHC could be used as a mean to manipulate the release behavior of growth factor from the DCPD/CHC hybrid coating. In addition, the BMP-2- and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited a sequential release of BMP-2 and TGF-β1 during the first and second two weeks, respectively. Importantly, the DCPD/CHC hybrid coating immersed in simulated body fluid gradually transformed to hydroxyapatite phase. Hence, the BMP-2 and PLA/TGF-β1 MSs-incorporated DCPD/CHC hybrid coating exhibited the best cell viability and the highest ALP activity after 14. days. The biocompatible hybrid coating with sequential release functionality proposed in the present study can enhance the osseointegration process and possess promising potential for clinical applications in bone implants and tissue engineering.

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KW - Electrodeposition

KW - TGF-β1

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