Growth arrest-specific 6 Protein in patients with sjögren syndrome: Determination of the plasma level and expression in the labial salivary gland

Chen Hung Chen, Hsiang Cheng Chen, Chi Ching Chang, Yi Jen Peng, Chien Hsing Lee, Yi Shing Shieh, Yi Jen Hung, Yuh Feng Lin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aims Growth arrest-specific protein 6 (Gas6) is a Vitamin K-dependent protein expressed by endothelial cells and leukocytes that are involved in cell survival, migration, and proliferation in response to inflammatory processes. The aim of this study was to assess the implications of Gas6 in Sjögren syndrome (SS) and its expression in the labial salivary gland. Methods and Results A total of 254 adults, including 159 with primary Sjögren syndrome (pSS), 34 with secondary Sjögren syndrome (sSS), and 61 normal controls, were recruited. Plasma Gas6 concentrations were determined, and Gas6 expressions in labial salivary gland (LSG) tissues from controls and pSS and sSS patients were also evaluated. Plasma Gas6 concentrations were significantly lower among patients with pSS than normal controls (13.5 ± 8.6 vs. 19.9 ± 13.4 ng/ml, p <0.001). There were, however, no significant differences in plasma Gas6 levels between pSS and sSS patients (13.5 ± 8.6 vs. 16.9 ± 11.2 ng/ml, p = 0.068). In multivariate logistic regression analysis, after adjustment for white blood cell count, hemoglobin level, platelet count, lymphocyte count, and C3 and C4 levels, lower plasma Gas6 concentrations were significantly associated with an increased risk of SS. Moreover, by using a semi-quantitative scale to evaluate Gas6 expression in LSG tissues, Gas6 expression was found to be markedly lower in LSG tissues from pSS patients than in tissues from normal controls. Conclusions Decreased plasma Gas6 concentration and LSG expression were associated with pSS. As such, Gas6 may represent a novel independent risk factor for pSS, with a potential role in salivary gland inflammation and dysfunction.

Original languageEnglish
Article number139955
JournalPLoS One
Volume10
Issue number10
DOIs
Publication statusPublished - Oct 7 2015

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lips
Lip
salivary glands
Salivary Glands
Plasmas
proteins
Tissue
growth arrest-specific protein 6
Cells
Sialadenitis
Lymphocytes
Vitamin K
vitamin K
Endothelial cells
blood platelet count
lymphocyte count
Platelets
Lymphocyte Count
Regression analysis
Platelet Count

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Growth arrest-specific 6 Protein in patients with sjögren syndrome : Determination of the plasma level and expression in the labial salivary gland. / Chen, Chen Hung; Chen, Hsiang Cheng; Chang, Chi Ching; Peng, Yi Jen; Lee, Chien Hsing; Shieh, Yi Shing; Hung, Yi Jen; Lin, Yuh Feng.

In: PLoS One, Vol. 10, No. 10, 139955, 07.10.2015.

Research output: Contribution to journalArticle

Chen, Chen Hung ; Chen, Hsiang Cheng ; Chang, Chi Ching ; Peng, Yi Jen ; Lee, Chien Hsing ; Shieh, Yi Shing ; Hung, Yi Jen ; Lin, Yuh Feng. / Growth arrest-specific 6 Protein in patients with sjögren syndrome : Determination of the plasma level and expression in the labial salivary gland. In: PLoS One. 2015 ; Vol. 10, No. 10.
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title = "Growth arrest-specific 6 Protein in patients with sj{\"o}gren syndrome: Determination of the plasma level and expression in the labial salivary gland",
abstract = "Aims Growth arrest-specific protein 6 (Gas6) is a Vitamin K-dependent protein expressed by endothelial cells and leukocytes that are involved in cell survival, migration, and proliferation in response to inflammatory processes. The aim of this study was to assess the implications of Gas6 in Sj{\"o}gren syndrome (SS) and its expression in the labial salivary gland. Methods and Results A total of 254 adults, including 159 with primary Sj{\"o}gren syndrome (pSS), 34 with secondary Sj{\"o}gren syndrome (sSS), and 61 normal controls, were recruited. Plasma Gas6 concentrations were determined, and Gas6 expressions in labial salivary gland (LSG) tissues from controls and pSS and sSS patients were also evaluated. Plasma Gas6 concentrations were significantly lower among patients with pSS than normal controls (13.5 ± 8.6 vs. 19.9 ± 13.4 ng/ml, p <0.001). There were, however, no significant differences in plasma Gas6 levels between pSS and sSS patients (13.5 ± 8.6 vs. 16.9 ± 11.2 ng/ml, p = 0.068). In multivariate logistic regression analysis, after adjustment for white blood cell count, hemoglobin level, platelet count, lymphocyte count, and C3 and C4 levels, lower plasma Gas6 concentrations were significantly associated with an increased risk of SS. Moreover, by using a semi-quantitative scale to evaluate Gas6 expression in LSG tissues, Gas6 expression was found to be markedly lower in LSG tissues from pSS patients than in tissues from normal controls. Conclusions Decreased plasma Gas6 concentration and LSG expression were associated with pSS. As such, Gas6 may represent a novel independent risk factor for pSS, with a potential role in salivary gland inflammation and dysfunction.",
author = "Chen, {Chen Hung} and Chen, {Hsiang Cheng} and Chang, {Chi Ching} and Peng, {Yi Jen} and Lee, {Chien Hsing} and Shieh, {Yi Shing} and Hung, {Yi Jen} and Lin, {Yuh Feng}",
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T2 - Determination of the plasma level and expression in the labial salivary gland

AU - Chen, Chen Hung

AU - Chen, Hsiang Cheng

AU - Chang, Chi Ching

AU - Peng, Yi Jen

AU - Lee, Chien Hsing

AU - Shieh, Yi Shing

AU - Hung, Yi Jen

AU - Lin, Yuh Feng

PY - 2015/10/7

Y1 - 2015/10/7

N2 - Aims Growth arrest-specific protein 6 (Gas6) is a Vitamin K-dependent protein expressed by endothelial cells and leukocytes that are involved in cell survival, migration, and proliferation in response to inflammatory processes. The aim of this study was to assess the implications of Gas6 in Sjögren syndrome (SS) and its expression in the labial salivary gland. Methods and Results A total of 254 adults, including 159 with primary Sjögren syndrome (pSS), 34 with secondary Sjögren syndrome (sSS), and 61 normal controls, were recruited. Plasma Gas6 concentrations were determined, and Gas6 expressions in labial salivary gland (LSG) tissues from controls and pSS and sSS patients were also evaluated. Plasma Gas6 concentrations were significantly lower among patients with pSS than normal controls (13.5 ± 8.6 vs. 19.9 ± 13.4 ng/ml, p <0.001). There were, however, no significant differences in plasma Gas6 levels between pSS and sSS patients (13.5 ± 8.6 vs. 16.9 ± 11.2 ng/ml, p = 0.068). In multivariate logistic regression analysis, after adjustment for white blood cell count, hemoglobin level, platelet count, lymphocyte count, and C3 and C4 levels, lower plasma Gas6 concentrations were significantly associated with an increased risk of SS. Moreover, by using a semi-quantitative scale to evaluate Gas6 expression in LSG tissues, Gas6 expression was found to be markedly lower in LSG tissues from pSS patients than in tissues from normal controls. Conclusions Decreased plasma Gas6 concentration and LSG expression were associated with pSS. As such, Gas6 may represent a novel independent risk factor for pSS, with a potential role in salivary gland inflammation and dysfunction.

AB - Aims Growth arrest-specific protein 6 (Gas6) is a Vitamin K-dependent protein expressed by endothelial cells and leukocytes that are involved in cell survival, migration, and proliferation in response to inflammatory processes. The aim of this study was to assess the implications of Gas6 in Sjögren syndrome (SS) and its expression in the labial salivary gland. Methods and Results A total of 254 adults, including 159 with primary Sjögren syndrome (pSS), 34 with secondary Sjögren syndrome (sSS), and 61 normal controls, were recruited. Plasma Gas6 concentrations were determined, and Gas6 expressions in labial salivary gland (LSG) tissues from controls and pSS and sSS patients were also evaluated. Plasma Gas6 concentrations were significantly lower among patients with pSS than normal controls (13.5 ± 8.6 vs. 19.9 ± 13.4 ng/ml, p <0.001). There were, however, no significant differences in plasma Gas6 levels between pSS and sSS patients (13.5 ± 8.6 vs. 16.9 ± 11.2 ng/ml, p = 0.068). In multivariate logistic regression analysis, after adjustment for white blood cell count, hemoglobin level, platelet count, lymphocyte count, and C3 and C4 levels, lower plasma Gas6 concentrations were significantly associated with an increased risk of SS. Moreover, by using a semi-quantitative scale to evaluate Gas6 expression in LSG tissues, Gas6 expression was found to be markedly lower in LSG tissues from pSS patients than in tissues from normal controls. Conclusions Decreased plasma Gas6 concentration and LSG expression were associated with pSS. As such, Gas6 may represent a novel independent risk factor for pSS, with a potential role in salivary gland inflammation and dysfunction.

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