GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function

Yi Wen Lin, Chun-Yao Huang, Yung Hsiang Chen, Chun-Ming Shih, Nai-Wen Tsao, FnmCheng Yen Lin, Nen-Chung Chang, Chien Sung Tsai, Hsiao Ya Tsai, Jui Chi Tsai, Po Hsun Huang, Chi Yuan Li, Feng-Yen Lin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The number and function of endothelial progenitor cells (EPCs) are sensitive to hyperglycemia, hypertension, and smoking in humans, which are also associated with the development of atherosclerosis. GroEL1 from Chlamydia pneumoniae has been found in atherosclerotic lesions and is related to atherosclerotic pathogenesis. However, the actual effects of GroEL1 on EPC function are unclear. In this study, we investigate the EPC function in GroEL1-administered hind limb-ischemic C57BL/B6 and C57BL/10ScNJ (a toll-like receptor 4 (TLR4) mutation) mice and human EPCs. In mice, laser Doppler imaging, flow cytometry, and immunohistochemistry were used to evaluate the degree of neo-vasculogenesis, circulating level of EPCs, and expression of CD34, vWF, and endothelial nitric oxide synthase (eNOS) in vessels. Blood flow in the ischemic limb was significantly impaired in C57BL/B6 but not C57BL/10ScNJ mice treated with GroEL1. Circulating EPCs were also decreased after GroEL1 administration in C57BL/B6 mice. Additionally, GroEL1 inhibited the expression of CD34 and eNOS in C57BL/B6 ischemic muscle. In vitro, GroEL1 impaired the capacity of differentiation, mobilization, tube formation, and migration of EPCs. GroEL1 increased senescence, which was mediated by caspases, p38 MAPK, and ERK1/2 signaling in EPCs. Furthermore, GroEL1 decreased integrin and E-selectin expression and induced inflammatory responses in EPCs. In conclusion, these findings suggest that TLR4 and impaired NO-related mechanisms could contribute to the reduced number and functional activity of EPCs in the presence of GroEL1 from C. pneumoniae.

Original languageEnglish
Article numbere84731
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 23 2013

Fingerprint

Chlamydophila pneumoniae
Chaperonin 60
Endothelial cells
angiogenesis
heat shock proteins
endothelial cells
stem cells
Toll-Like Receptor 4
Nitric Oxide Synthase Type III
mice
limbs (animal)
Inbred C57BL Mouse
Extremities
Endothelial Progenitor Cells
E-Selectin
Flow cytometry
caspases
integrins
p38 Mitogen-Activated Protein Kinases
hyperglycemia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function. / Lin, Yi Wen; Huang, Chun-Yao; Chen, Yung Hsiang; Shih, Chun-Ming; Tsao, Nai-Wen; Lin, FnmCheng Yen; Chang, Nen-Chung; Tsai, Chien Sung; Tsai, Hsiao Ya; Tsai, Jui Chi; Huang, Po Hsun; Li, Chi Yuan; Lin, Feng-Yen.

In: PLoS One, Vol. 8, No. 12, e84731, 23.12.2013.

Research output: Contribution to journalArticle

Lin, Yi Wen ; Huang, Chun-Yao ; Chen, Yung Hsiang ; Shih, Chun-Ming ; Tsao, Nai-Wen ; Lin, FnmCheng Yen ; Chang, Nen-Chung ; Tsai, Chien Sung ; Tsai, Hsiao Ya ; Tsai, Jui Chi ; Huang, Po Hsun ; Li, Chi Yuan ; Lin, Feng-Yen. / GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function. In: PLoS One. 2013 ; Vol. 8, No. 12.
@article{9f204834f0b84a0abc2bd2e92b49eeea,
title = "GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function",
abstract = "The number and function of endothelial progenitor cells (EPCs) are sensitive to hyperglycemia, hypertension, and smoking in humans, which are also associated with the development of atherosclerosis. GroEL1 from Chlamydia pneumoniae has been found in atherosclerotic lesions and is related to atherosclerotic pathogenesis. However, the actual effects of GroEL1 on EPC function are unclear. In this study, we investigate the EPC function in GroEL1-administered hind limb-ischemic C57BL/B6 and C57BL/10ScNJ (a toll-like receptor 4 (TLR4) mutation) mice and human EPCs. In mice, laser Doppler imaging, flow cytometry, and immunohistochemistry were used to evaluate the degree of neo-vasculogenesis, circulating level of EPCs, and expression of CD34, vWF, and endothelial nitric oxide synthase (eNOS) in vessels. Blood flow in the ischemic limb was significantly impaired in C57BL/B6 but not C57BL/10ScNJ mice treated with GroEL1. Circulating EPCs were also decreased after GroEL1 administration in C57BL/B6 mice. Additionally, GroEL1 inhibited the expression of CD34 and eNOS in C57BL/B6 ischemic muscle. In vitro, GroEL1 impaired the capacity of differentiation, mobilization, tube formation, and migration of EPCs. GroEL1 increased senescence, which was mediated by caspases, p38 MAPK, and ERK1/2 signaling in EPCs. Furthermore, GroEL1 decreased integrin and E-selectin expression and induced inflammatory responses in EPCs. In conclusion, these findings suggest that TLR4 and impaired NO-related mechanisms could contribute to the reduced number and functional activity of EPCs in the presence of GroEL1 from C. pneumoniae.",
author = "Lin, {Yi Wen} and Chun-Yao Huang and Chen, {Yung Hsiang} and Chun-Ming Shih and Nai-Wen Tsao and Lin, {FnmCheng Yen} and Nen-Chung Chang and Tsai, {Chien Sung} and Tsai, {Hsiao Ya} and Tsai, {Jui Chi} and Huang, {Po Hsun} and Li, {Chi Yuan} and Feng-Yen Lin",
year = "2013",
month = "12",
day = "23",
doi = "10.1371/journal.pone.0084731",
language = "English",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function

AU - Lin, Yi Wen

AU - Huang, Chun-Yao

AU - Chen, Yung Hsiang

AU - Shih, Chun-Ming

AU - Tsao, Nai-Wen

AU - Lin, FnmCheng Yen

AU - Chang, Nen-Chung

AU - Tsai, Chien Sung

AU - Tsai, Hsiao Ya

AU - Tsai, Jui Chi

AU - Huang, Po Hsun

AU - Li, Chi Yuan

AU - Lin, Feng-Yen

PY - 2013/12/23

Y1 - 2013/12/23

N2 - The number and function of endothelial progenitor cells (EPCs) are sensitive to hyperglycemia, hypertension, and smoking in humans, which are also associated with the development of atherosclerosis. GroEL1 from Chlamydia pneumoniae has been found in atherosclerotic lesions and is related to atherosclerotic pathogenesis. However, the actual effects of GroEL1 on EPC function are unclear. In this study, we investigate the EPC function in GroEL1-administered hind limb-ischemic C57BL/B6 and C57BL/10ScNJ (a toll-like receptor 4 (TLR4) mutation) mice and human EPCs. In mice, laser Doppler imaging, flow cytometry, and immunohistochemistry were used to evaluate the degree of neo-vasculogenesis, circulating level of EPCs, and expression of CD34, vWF, and endothelial nitric oxide synthase (eNOS) in vessels. Blood flow in the ischemic limb was significantly impaired in C57BL/B6 but not C57BL/10ScNJ mice treated with GroEL1. Circulating EPCs were also decreased after GroEL1 administration in C57BL/B6 mice. Additionally, GroEL1 inhibited the expression of CD34 and eNOS in C57BL/B6 ischemic muscle. In vitro, GroEL1 impaired the capacity of differentiation, mobilization, tube formation, and migration of EPCs. GroEL1 increased senescence, which was mediated by caspases, p38 MAPK, and ERK1/2 signaling in EPCs. Furthermore, GroEL1 decreased integrin and E-selectin expression and induced inflammatory responses in EPCs. In conclusion, these findings suggest that TLR4 and impaired NO-related mechanisms could contribute to the reduced number and functional activity of EPCs in the presence of GroEL1 from C. pneumoniae.

AB - The number and function of endothelial progenitor cells (EPCs) are sensitive to hyperglycemia, hypertension, and smoking in humans, which are also associated with the development of atherosclerosis. GroEL1 from Chlamydia pneumoniae has been found in atherosclerotic lesions and is related to atherosclerotic pathogenesis. However, the actual effects of GroEL1 on EPC function are unclear. In this study, we investigate the EPC function in GroEL1-administered hind limb-ischemic C57BL/B6 and C57BL/10ScNJ (a toll-like receptor 4 (TLR4) mutation) mice and human EPCs. In mice, laser Doppler imaging, flow cytometry, and immunohistochemistry were used to evaluate the degree of neo-vasculogenesis, circulating level of EPCs, and expression of CD34, vWF, and endothelial nitric oxide synthase (eNOS) in vessels. Blood flow in the ischemic limb was significantly impaired in C57BL/B6 but not C57BL/10ScNJ mice treated with GroEL1. Circulating EPCs were also decreased after GroEL1 administration in C57BL/B6 mice. Additionally, GroEL1 inhibited the expression of CD34 and eNOS in C57BL/B6 ischemic muscle. In vitro, GroEL1 impaired the capacity of differentiation, mobilization, tube formation, and migration of EPCs. GroEL1 increased senescence, which was mediated by caspases, p38 MAPK, and ERK1/2 signaling in EPCs. Furthermore, GroEL1 decreased integrin and E-selectin expression and induced inflammatory responses in EPCs. In conclusion, these findings suggest that TLR4 and impaired NO-related mechanisms could contribute to the reduced number and functional activity of EPCs in the presence of GroEL1 from C. pneumoniae.

UR - http://www.scopus.com/inward/record.url?scp=84893452214&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893452214&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0084731

DO - 10.1371/journal.pone.0084731

M3 - Article

C2 - 24376840

AN - SCOPUS:84893452214

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e84731

ER -