Granulocyte-macrophage colony-forming-unit assay and liquid suspension culture of bone marrow and peripheral blood in newly diagnosed childhood acute lymphoblastic leukemia

Der Cherng Liang, Shu Huey Chen, Hsi Che Liu, Shu Fang Yu, Mei Chuang Kuo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Granulocyte-macrophage colony-forming-unit (CFU-GM)-assays and/or liquid suspension cultures of bone marrow (BM) and peripheral blood (PB), using human placental conditioned medium as a source of colony-stimulating activity, were performed in 125 children with newly diagnosed acute lymphoblastic leukemia (ALL). Unlike the previous small series which invariably showed poor BM granulocyte-macrophage colony and cluster formations, we found 3 growth patterns. A poor-growth pattern was seen in 101 patients. A low-growth pattern with BM colony and cluster formations in the lower range of the normal limits was observed in 6 patients. All 6 patients had low-risk ALL, and the in vitro growth may reflect the residual normal CFU-GM in BM. Thirdly an over-growth pattern was noted in one patient with refractory ALL. In 49 cases where liquid suspension cultures of BM were performed, 4 had a normal-growth pattern and 42 had apoptotic cells and/or few residual granulocytic/monocytic cells after 7 days of culture. However, in both B cell ALL cases and in 1 refractory T cell ALL case with hyperleukocytosis, repetitive liquid suspension cultures of BM and PB consistently revealed an extensive growth of lymphoblasts. Thus, an over-growth pattern in either the BM CFU-GM assay or liquid suspension culture of BM or PB in children with ALL at diagnosis may indicate a refractory or aggressive disease. A low-growth pattern in the BM CFU-GM assay in children with ALL at diagnosis probably predicts a favorable outcome.

Original languageEnglish
Pages (from-to)12-16
Number of pages5
JournalActa Haematologica
Volume99
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Colony-Forming Units Assay
Granulocyte-Macrophage Progenitor Cells
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Suspensions
Bone Marrow
Growth
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Conditioned Culture Medium
Granulocytes
Reference Values
B-Lymphocytes
Macrophages

Keywords

  • Bone marrow
  • Childhood acute lymphoblastic leukemia
  • Granulocyte-macrophage colony-forming-unit assay
  • Liquid suspension culture
  • Peripheral blood

ASJC Scopus subject areas

  • Hematology

Cite this

Granulocyte-macrophage colony-forming-unit assay and liquid suspension culture of bone marrow and peripheral blood in newly diagnosed childhood acute lymphoblastic leukemia. / Liang, Der Cherng; Chen, Shu Huey; Liu, Hsi Che; Yu, Shu Fang; Kuo, Mei Chuang.

In: Acta Haematologica, Vol. 99, No. 1, 1998, p. 12-16.

Research output: Contribution to journalArticle

@article{f842e476f4a34bf58b10ae481cc2c54a,
title = "Granulocyte-macrophage colony-forming-unit assay and liquid suspension culture of bone marrow and peripheral blood in newly diagnosed childhood acute lymphoblastic leukemia",
abstract = "Granulocyte-macrophage colony-forming-unit (CFU-GM)-assays and/or liquid suspension cultures of bone marrow (BM) and peripheral blood (PB), using human placental conditioned medium as a source of colony-stimulating activity, were performed in 125 children with newly diagnosed acute lymphoblastic leukemia (ALL). Unlike the previous small series which invariably showed poor BM granulocyte-macrophage colony and cluster formations, we found 3 growth patterns. A poor-growth pattern was seen in 101 patients. A low-growth pattern with BM colony and cluster formations in the lower range of the normal limits was observed in 6 patients. All 6 patients had low-risk ALL, and the in vitro growth may reflect the residual normal CFU-GM in BM. Thirdly an over-growth pattern was noted in one patient with refractory ALL. In 49 cases where liquid suspension cultures of BM were performed, 4 had a normal-growth pattern and 42 had apoptotic cells and/or few residual granulocytic/monocytic cells after 7 days of culture. However, in both B cell ALL cases and in 1 refractory T cell ALL case with hyperleukocytosis, repetitive liquid suspension cultures of BM and PB consistently revealed an extensive growth of lymphoblasts. Thus, an over-growth pattern in either the BM CFU-GM assay or liquid suspension culture of BM or PB in children with ALL at diagnosis may indicate a refractory or aggressive disease. A low-growth pattern in the BM CFU-GM assay in children with ALL at diagnosis probably predicts a favorable outcome.",
keywords = "Bone marrow, Childhood acute lymphoblastic leukemia, Granulocyte-macrophage colony-forming-unit assay, Liquid suspension culture, Peripheral blood",
author = "Liang, {Der Cherng} and Chen, {Shu Huey} and Liu, {Hsi Che} and Yu, {Shu Fang} and Kuo, {Mei Chuang}",
year = "1998",
doi = "10.1159/000040708",
language = "English",
volume = "99",
pages = "12--16",
journal = "Acta Haematologica",
issn = "0001-5792",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Granulocyte-macrophage colony-forming-unit assay and liquid suspension culture of bone marrow and peripheral blood in newly diagnosed childhood acute lymphoblastic leukemia

AU - Liang, Der Cherng

AU - Chen, Shu Huey

AU - Liu, Hsi Che

AU - Yu, Shu Fang

AU - Kuo, Mei Chuang

PY - 1998

Y1 - 1998

N2 - Granulocyte-macrophage colony-forming-unit (CFU-GM)-assays and/or liquid suspension cultures of bone marrow (BM) and peripheral blood (PB), using human placental conditioned medium as a source of colony-stimulating activity, were performed in 125 children with newly diagnosed acute lymphoblastic leukemia (ALL). Unlike the previous small series which invariably showed poor BM granulocyte-macrophage colony and cluster formations, we found 3 growth patterns. A poor-growth pattern was seen in 101 patients. A low-growth pattern with BM colony and cluster formations in the lower range of the normal limits was observed in 6 patients. All 6 patients had low-risk ALL, and the in vitro growth may reflect the residual normal CFU-GM in BM. Thirdly an over-growth pattern was noted in one patient with refractory ALL. In 49 cases where liquid suspension cultures of BM were performed, 4 had a normal-growth pattern and 42 had apoptotic cells and/or few residual granulocytic/monocytic cells after 7 days of culture. However, in both B cell ALL cases and in 1 refractory T cell ALL case with hyperleukocytosis, repetitive liquid suspension cultures of BM and PB consistently revealed an extensive growth of lymphoblasts. Thus, an over-growth pattern in either the BM CFU-GM assay or liquid suspension culture of BM or PB in children with ALL at diagnosis may indicate a refractory or aggressive disease. A low-growth pattern in the BM CFU-GM assay in children with ALL at diagnosis probably predicts a favorable outcome.

AB - Granulocyte-macrophage colony-forming-unit (CFU-GM)-assays and/or liquid suspension cultures of bone marrow (BM) and peripheral blood (PB), using human placental conditioned medium as a source of colony-stimulating activity, were performed in 125 children with newly diagnosed acute lymphoblastic leukemia (ALL). Unlike the previous small series which invariably showed poor BM granulocyte-macrophage colony and cluster formations, we found 3 growth patterns. A poor-growth pattern was seen in 101 patients. A low-growth pattern with BM colony and cluster formations in the lower range of the normal limits was observed in 6 patients. All 6 patients had low-risk ALL, and the in vitro growth may reflect the residual normal CFU-GM in BM. Thirdly an over-growth pattern was noted in one patient with refractory ALL. In 49 cases where liquid suspension cultures of BM were performed, 4 had a normal-growth pattern and 42 had apoptotic cells and/or few residual granulocytic/monocytic cells after 7 days of culture. However, in both B cell ALL cases and in 1 refractory T cell ALL case with hyperleukocytosis, repetitive liquid suspension cultures of BM and PB consistently revealed an extensive growth of lymphoblasts. Thus, an over-growth pattern in either the BM CFU-GM assay or liquid suspension culture of BM or PB in children with ALL at diagnosis may indicate a refractory or aggressive disease. A low-growth pattern in the BM CFU-GM assay in children with ALL at diagnosis probably predicts a favorable outcome.

KW - Bone marrow

KW - Childhood acute lymphoblastic leukemia

KW - Granulocyte-macrophage colony-forming-unit assay

KW - Liquid suspension culture

KW - Peripheral blood

UR - http://www.scopus.com/inward/record.url?scp=0031976098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031976098&partnerID=8YFLogxK

U2 - 10.1159/000040708

DO - 10.1159/000040708

M3 - Article

C2 - 9490559

AN - SCOPUS:0031976098

VL - 99

SP - 12

EP - 16

JO - Acta Haematologica

JF - Acta Haematologica

SN - 0001-5792

IS - 1

ER -