GPX2 underexpression indicates poor prognosis in patients with urothelial carcinomas of the upper urinary tract and urinary bladder

I. Wei Chang, Victor Chia Hsiang Lin, Chih Hsin Hung, Hua Pin Wang, Yung Yao Lin, Wen Jeng Wu, Chun Nung Huang, Ching Chia Li, Wei Ming Li, Jui Yu Wu, Chien Feng Li

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14 Citations (Scopus)

Abstract

Purpose: Oxidative stress is believed to be one of the important etiologies in carcinogenesis that has not been systemically investigated in urothelial carcinoma (UC). Through data mining from a published transcriptomic database of UC of urinary bladders (UBUCs) (GSE31684), glutathione peroxidase 2 (GPX2) was identified as the most significant downregulated gene among those response to oxidative stress (GO:0006979). We therefore analyze GPX2 transcript and protein expressions and its clinicopathological significance. Methods: Real-time RT-PCR assay was used to detect GPX2 mRNA level in 20 fresh UBUC specimens. Immunohistochemistry was used to determine GPX2 protein expression in 340 urothelial carcinomas of upper tracts (UTUCs) and 295 UBUCs with mean/median follow-up of 44.7/38.9 and 30.8/23.1 months, respectively. Its expression status was further correlated with clinicopathological features and evaluated for its impact on disease-specific survival and metastasis-free survival (MeFS). Results: Decrease in GPX2 transcript level was associated with both higher pT and positive nodal status in 20 UBUCs (all p 

Original languageEnglish
Pages (from-to)1777-1789
Number of pages13
JournalWorld Journal of Urology
Volume33
Issue number11
DOIs
Publication statusPublished - Mar 27 2015

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Keywords

  • Glutathione peroxidase 2
  • GPX2
  • Prognosis
  • Urothelial carcinoma

ASJC Scopus subject areas

  • Urology

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