GPX2 underexpression indicates poor prognosis in patients with urothelial carcinomas of the upper urinary tract and urinary bladder

I. Wei Chang, Victor Chia Hsiang Lin, Chih Hsin Hung, Hua Pin Wang, Yung Yao Lin, Wen Jeng Wu, Chun Nung Huang, Ching Chia Li, Wei Ming Li, Jui Yu Wu, Chien Feng Li

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: Oxidative stress is believed to be one of the important etiologies in carcinogenesis that has not been systemically investigated in urothelial carcinoma (UC). Through data mining from a published transcriptomic database of UC of urinary bladders (UBUCs) (GSE31684), glutathione peroxidase 2 (GPX2) was identified as the most significant downregulated gene among those response to oxidative stress (GO:0006979). We therefore analyze GPX2 transcript and protein expressions and its clinicopathological significance. Methods: Real-time RT-PCR assay was used to detect GPX2 mRNA level in 20 fresh UBUC specimens. Immunohistochemistry was used to determine GPX2 protein expression in 340 urothelial carcinomas of upper tracts (UTUCs) and 295 UBUCs with mean/median follow-up of 44.7/38.9 and 30.8/23.1 months, respectively. Its expression status was further correlated with clinicopathological features and evaluated for its impact on disease-specific survival and metastasis-free survival (MeFS). Results: Decrease in GPX2 transcript level was associated with both higher pT and positive nodal status in 20 UBUCs (all p 

Original languageEnglish
Pages (from-to)1777-1789
Number of pages13
JournalWorld Journal of Urology
Volume33
Issue number11
DOIs
Publication statusPublished - Mar 27 2015

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Glutathione Peroxidase
Urinary Tract
Urinary Bladder
Carcinoma
Oxidative Stress
Data Mining
Survival
Real-Time Polymerase Chain Reaction
Carcinogenesis
Proteins
Down-Regulation
Immunohistochemistry
Databases
Neoplasm Metastasis
Messenger RNA
Genes

Keywords

  • Glutathione peroxidase 2
  • GPX2
  • Prognosis
  • Urothelial carcinoma

ASJC Scopus subject areas

  • Urology

Cite this

GPX2 underexpression indicates poor prognosis in patients with urothelial carcinomas of the upper urinary tract and urinary bladder. / Chang, I. Wei; Lin, Victor Chia Hsiang; Hung, Chih Hsin; Wang, Hua Pin; Lin, Yung Yao; Wu, Wen Jeng; Huang, Chun Nung; Li, Ching Chia; Li, Wei Ming; Wu, Jui Yu; Li, Chien Feng.

In: World Journal of Urology, Vol. 33, No. 11, 27.03.2015, p. 1777-1789.

Research output: Contribution to journalArticle

Chang, I. Wei ; Lin, Victor Chia Hsiang ; Hung, Chih Hsin ; Wang, Hua Pin ; Lin, Yung Yao ; Wu, Wen Jeng ; Huang, Chun Nung ; Li, Ching Chia ; Li, Wei Ming ; Wu, Jui Yu ; Li, Chien Feng. / GPX2 underexpression indicates poor prognosis in patients with urothelial carcinomas of the upper urinary tract and urinary bladder. In: World Journal of Urology. 2015 ; Vol. 33, No. 11. pp. 1777-1789.
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AU - Wang, Hua Pin

AU - Lin, Yung Yao

AU - Wu, Wen Jeng

AU - Huang, Chun Nung

AU - Li, Ching Chia

AU - Li, Wei Ming

AU - Wu, Jui Yu

AU - Li, Chien Feng

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