Abstract
Matrix-associated autologous chondrocyte implantation (MACI) is an effective treatment for full-thickness cartilage and osteochondral lesions with encouraging outcomes. However, problems include abnormal growth of chondrocytes during cultivation, cell dedifferentiation, and abnormally regenerated cartilage. A matrix that provides a physicochemical and biological microenvironment for restoring hypertrophic chondrocytes would be beneficial for MACI. Accordingly, this study evaluates the feasibility of using an injectable glycosaminoglycan (GAG)/chitosan hydrogel for MACI. Chitosan gel was prepared and GAGs (hyaluronan and chondroitin-6-sulfate) were added to fabricate a GAG/chitosan matrix. Porcine chondrocytes were isolated from articular cartilage and encapsulated within the GAG/chitosan matrix. Cell viability, material-mediated cytotoxicity, cellular proliferation, collagen production, GAG content, and mRNA gene expression patterns of the chondrocytes were evaluated. The cell viability and material-mediated cytotoxicity assay results show that the GAG/chitosan hydrogel has good biocompatibility. Chondrocytes cultured within the matrix had a slower proliferation but higher GAG production compared to those obtained for a monolayer culture. Real-time polymerase chain reaction results show that the mRNA expression of type II collagen was up-regulated but types I and X collagens were down-regulated. This study demonstrates that incorporating GAGs into a chitosan matrix maintains the normal phenotype of chondrocytes, making the GAG/chitosan matrix a candidate for MACI.
| Original language | English |
|---|---|
| Pages (from-to) | 211-217 |
| Number of pages | 7 |
| Journal | Journal of Medical and Biological Engineering |
| Volume | 34 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Autologous chondrocyte implantation
- Chitosan hydrogel
- Chondrocyte
- Glycosaminoglycan
ASJC Scopus subject areas
- Biomedical Engineering
- Medicine(all)
Cite this
Glycosaminoglycan/chitosan hydrogel for matrix-associated autologous chondrocyte implantation : An in vitro study. / Fan, Fang Yu; Chiu, Chien Chang; Tseng, Ching Li; Lee, Hsuan Shu; Pan, Yung Ning; Yang, Kai Chiang.
In: Journal of Medical and Biological Engineering, Vol. 34, No. 3, 2014, p. 211-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycosaminoglycan/chitosan hydrogel for matrix-associated autologous chondrocyte implantation
T2 - An in vitro study
AU - Fan, Fang Yu
AU - Chiu, Chien Chang
AU - Tseng, Ching Li
AU - Lee, Hsuan Shu
AU - Pan, Yung Ning
AU - Yang, Kai Chiang
PY - 2014
Y1 - 2014
N2 - Matrix-associated autologous chondrocyte implantation (MACI) is an effective treatment for full-thickness cartilage and osteochondral lesions with encouraging outcomes. However, problems include abnormal growth of chondrocytes during cultivation, cell dedifferentiation, and abnormally regenerated cartilage. A matrix that provides a physicochemical and biological microenvironment for restoring hypertrophic chondrocytes would be beneficial for MACI. Accordingly, this study evaluates the feasibility of using an injectable glycosaminoglycan (GAG)/chitosan hydrogel for MACI. Chitosan gel was prepared and GAGs (hyaluronan and chondroitin-6-sulfate) were added to fabricate a GAG/chitosan matrix. Porcine chondrocytes were isolated from articular cartilage and encapsulated within the GAG/chitosan matrix. Cell viability, material-mediated cytotoxicity, cellular proliferation, collagen production, GAG content, and mRNA gene expression patterns of the chondrocytes were evaluated. The cell viability and material-mediated cytotoxicity assay results show that the GAG/chitosan hydrogel has good biocompatibility. Chondrocytes cultured within the matrix had a slower proliferation but higher GAG production compared to those obtained for a monolayer culture. Real-time polymerase chain reaction results show that the mRNA expression of type II collagen was up-regulated but types I and X collagens were down-regulated. This study demonstrates that incorporating GAGs into a chitosan matrix maintains the normal phenotype of chondrocytes, making the GAG/chitosan matrix a candidate for MACI.
AB - Matrix-associated autologous chondrocyte implantation (MACI) is an effective treatment for full-thickness cartilage and osteochondral lesions with encouraging outcomes. However, problems include abnormal growth of chondrocytes during cultivation, cell dedifferentiation, and abnormally regenerated cartilage. A matrix that provides a physicochemical and biological microenvironment for restoring hypertrophic chondrocytes would be beneficial for MACI. Accordingly, this study evaluates the feasibility of using an injectable glycosaminoglycan (GAG)/chitosan hydrogel for MACI. Chitosan gel was prepared and GAGs (hyaluronan and chondroitin-6-sulfate) were added to fabricate a GAG/chitosan matrix. Porcine chondrocytes were isolated from articular cartilage and encapsulated within the GAG/chitosan matrix. Cell viability, material-mediated cytotoxicity, cellular proliferation, collagen production, GAG content, and mRNA gene expression patterns of the chondrocytes were evaluated. The cell viability and material-mediated cytotoxicity assay results show that the GAG/chitosan hydrogel has good biocompatibility. Chondrocytes cultured within the matrix had a slower proliferation but higher GAG production compared to those obtained for a monolayer culture. Real-time polymerase chain reaction results show that the mRNA expression of type II collagen was up-regulated but types I and X collagens were down-regulated. This study demonstrates that incorporating GAGs into a chitosan matrix maintains the normal phenotype of chondrocytes, making the GAG/chitosan matrix a candidate for MACI.
KW - Autologous chondrocyte implantation
KW - Chitosan hydrogel
KW - Chondrocyte
KW - Glycosaminoglycan
UR - http://www.scopus.com/inward/record.url?scp=84903775820&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903775820&partnerID=8YFLogxK
U2 - 10.5405/jmbe.1516
DO - 10.5405/jmbe.1516
M3 - Article
AN - SCOPUS:84903775820
VL - 34
SP - 211
EP - 217
JO - Journal of Medical and Biological Engineering
JF - Journal of Medical and Biological Engineering
SN - 1609-0985
IS - 3
ER -