Glycosaminoglycan-based hybrid hydrogel encapsulated with polyelectrolyte complex nanoparticles for endogenous stem cell regulation in central nervous system regeneration

Wei Hong Jian, Huan Chih Wang, Chen Hsiang Kuan, Ming Hong Chen, Hsi Chin Wu, Jui Sheng Sun, Tzu Wei Wang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The poor regenerative capability of stem cell transplantation in the central nervous system limits their therapeutic efficacy in brain injuries. The sustained inflammatory response, lack of structural support, and trophic factors deficiency restrain the integration and long-term survival of stem cells. Instead of exogenous stem cell therapy, here we described the synthesis of nanohybrid hydrogel containing sulfated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) to mimic the brain extracellular matrix and control the delivery of stromal-derived factor-1α (SDF-1α) and basic fibroblast factor (bFGF) in response to matrix metalloproteinase (MMP) for recruiting endogenous neural stem cells (NSC) and regulating their cellular fate. Bioactive factors are delivered by electrostatic sequestration on PCN to amplify the signaling of SDF-1α and bFGF to regulate NSC in vitro. In in vivo ischemic stroke model, the factors promoted neurological behavior recovery by enhancing neurogenesis and angiogenesis. These combined strategies may be applied for other tissue regenerations by regulating endogenous progenitors through the delivery of different kinds of glycosaminoglycan-binding molecules.

Original languageEnglish
Pages (from-to)17-30
Number of pages14
JournalBiomaterials
Volume174
DOIs
Publication statusPublished - Aug 2018
Externally publishedYes

Keywords

  • Brain
  • Glycosaminoglycan
  • Growth factors
  • Hydrogel
  • Neural stem cell
  • Polyelectrolyte complex nanoparticle

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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