@article{24551ab244cf469ca3a5aa3f9950e680,
title = "Glycosaminoglycan-based hybrid hydrogel encapsulated with polyelectrolyte complex nanoparticles for endogenous stem cell regulation in central nervous system regeneration",
abstract = "The poor regenerative capability of stem cell transplantation in the central nervous system limits their therapeutic efficacy in brain injuries. The sustained inflammatory response, lack of structural support, and trophic factors deficiency restrain the integration and long-term survival of stem cells. Instead of exogenous stem cell therapy, here we described the synthesis of nanohybrid hydrogel containing sulfated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) to mimic the brain extracellular matrix and control the delivery of stromal-derived factor-1α (SDF-1α) and basic fibroblast factor (bFGF) in response to matrix metalloproteinase (MMP) for recruiting endogenous neural stem cells (NSC) and regulating their cellular fate. Bioactive factors are delivered by electrostatic sequestration on PCN to amplify the signaling of SDF-1α and bFGF to regulate NSC in vitro. In in vivo ischemic stroke model, the factors promoted neurological behavior recovery by enhancing neurogenesis and angiogenesis. These combined strategies may be applied for other tissue regenerations by regulating endogenous progenitors through the delivery of different kinds of glycosaminoglycan-binding molecules.",
keywords = "Brain, Glycosaminoglycan, Growth factors, Hydrogel, Neural stem cell, Polyelectrolyte complex nanoparticle",
author = "Jian, {Wei Hong} and Wang, {Huan Chih} and Kuan, {Chen Hsiang} and Chen, {Ming Hong} and Wu, {Hsi Chin} and Sun, {Jui Sheng} and Wang, {Tzu Wei}",
note = "Funding Information: We thank for the technical assistance from Magnetic Resonance Imaging Core Lab of Molecular Imaging Center in National Taiwan University (NTU) and IBEN Service in National Health Research Institutes for MR scanning. We are also grateful to the Technology Commons, College of Life Science, NTU for technical assistance with transmission electron microscopy (TEM), and the help of Instrument Center in National Tsing Hua University (NTHU) with NMR analysis. We also acknowledge Zheng-Wei Lu (Department of Chemical Engineering, NTHU) for assistance with the rheology analysis, and Kun-I Chao (Department of Biomedical Engineering and Environmental Sciences, NTHU) for helpful suggestions in MRI analysis. Funding was provided by the Taiwan Ministry of Science and Technology ( MOST-104-2628-E-007 -002 -MY3 , MOST-104-2221-E-007 -140 -MY2 , MOST-106-2622-E-007-003 -CC2 ). Funding Information: We thank for the technical assistance from Magnetic Resonance Imaging Core Lab of Molecular Imaging Center in National Taiwan University (NTU) and IBEN Service in National Health Research Institutes for MR scanning. We are also grateful to the Technology Commons, College of Life Science, NTU for technical assistance with transmission electron microscopy (TEM), and the help of Instrument Center in National Tsing Hua University (NTHU) with NMR analysis. We also acknowledge Zheng-Wei Lu (Department of Chemical Engineering, NTHU) for assistance with the rheology analysis, and Kun-I Chao (Department of Biomedical Engineering and Environmental Sciences, NTHU) for helpful suggestions in MRI analysis. Funding was provided by the Taiwan Ministry of Science and Technology (MOST-104-2628-E-007 -002 -MY3, MOST-104-2221-E-007 -140 -MY2, MOST-106-2622-E-007-003 -CC2).",
year = "2018",
month = aug,
doi = "10.1016/j.biomaterials.2018.05.009",
language = "English",
volume = "174",
pages = "17--30",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Science Ltd",
}