Abstract
The poor regenerative capability of stem cell transplantation in the central nervous system limits their therapeutic efficacy in brain injuries. The sustained inflammatory response, lack of structural support, and trophic factors deficiency restrain the integration and long-term survival of stem cells. Instead of exogenous stem cell therapy, here we described the synthesis of nanohybrid hydrogel containing sulfated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) to mimic the brain extracellular matrix and control the delivery of stromal-derived factor-1α (SDF-1α) and basic fibroblast factor (bFGF) in response to matrix metalloproteinase (MMP) for recruiting endogenous neural stem cells (NSC) and regulating their cellular fate. Bioactive factors are delivered by electrostatic sequestration on PCN to amplify the signaling of SDF-1α and bFGF to regulate NSC in vitro. In in vivo ischemic stroke model, the factors promoted neurological behavior recovery by enhancing neurogenesis and angiogenesis. These combined strategies may be applied for other tissue regenerations by regulating endogenous progenitors through the delivery of different kinds of glycosaminoglycan-binding molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 17-30 |
| Number of pages | 14 |
| Journal | Biomaterials |
| Volume | 174 |
| DOIs | |
| Publication status | Published - Aug 2018 |
| Externally published | Yes |
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Keywords
- Brain
- Glycosaminoglycan
- Growth factors
- Hydrogel
- Neural stem cell
- Polyelectrolyte complex nanoparticle
ASJC Scopus subject areas
- Bioengineering
- Ceramics and Composites
- Biophysics
- Biomaterials
- Mechanics of Materials
Cite this
Glycosaminoglycan-based hybrid hydrogel encapsulated with polyelectrolyte complex nanoparticles for endogenous stem cell regulation in central nervous system regeneration. / Jian, Wei Hong; Wang, Huan Chih; Kuan, Chen Hsiang; Chen, Ming Hong; Wu, Hsi Chin; Sun, Jui Sheng; Wang, Tzu Wei.
In: Biomaterials, Vol. 174, 08.2018, p. 17-30.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycosaminoglycan-based hybrid hydrogel encapsulated with polyelectrolyte complex nanoparticles for endogenous stem cell regulation in central nervous system regeneration
AU - Jian, Wei Hong
AU - Wang, Huan Chih
AU - Kuan, Chen Hsiang
AU - Chen, Ming Hong
AU - Wu, Hsi Chin
AU - Sun, Jui Sheng
AU - Wang, Tzu Wei
PY - 2018/8
Y1 - 2018/8
N2 - The poor regenerative capability of stem cell transplantation in the central nervous system limits their therapeutic efficacy in brain injuries. The sustained inflammatory response, lack of structural support, and trophic factors deficiency restrain the integration and long-term survival of stem cells. Instead of exogenous stem cell therapy, here we described the synthesis of nanohybrid hydrogel containing sulfated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) to mimic the brain extracellular matrix and control the delivery of stromal-derived factor-1α (SDF-1α) and basic fibroblast factor (bFGF) in response to matrix metalloproteinase (MMP) for recruiting endogenous neural stem cells (NSC) and regulating their cellular fate. Bioactive factors are delivered by electrostatic sequestration on PCN to amplify the signaling of SDF-1α and bFGF to regulate NSC in vitro. In in vivo ischemic stroke model, the factors promoted neurological behavior recovery by enhancing neurogenesis and angiogenesis. These combined strategies may be applied for other tissue regenerations by regulating endogenous progenitors through the delivery of different kinds of glycosaminoglycan-binding molecules.
AB - The poor regenerative capability of stem cell transplantation in the central nervous system limits their therapeutic efficacy in brain injuries. The sustained inflammatory response, lack of structural support, and trophic factors deficiency restrain the integration and long-term survival of stem cells. Instead of exogenous stem cell therapy, here we described the synthesis of nanohybrid hydrogel containing sulfated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) to mimic the brain extracellular matrix and control the delivery of stromal-derived factor-1α (SDF-1α) and basic fibroblast factor (bFGF) in response to matrix metalloproteinase (MMP) for recruiting endogenous neural stem cells (NSC) and regulating their cellular fate. Bioactive factors are delivered by electrostatic sequestration on PCN to amplify the signaling of SDF-1α and bFGF to regulate NSC in vitro. In in vivo ischemic stroke model, the factors promoted neurological behavior recovery by enhancing neurogenesis and angiogenesis. These combined strategies may be applied for other tissue regenerations by regulating endogenous progenitors through the delivery of different kinds of glycosaminoglycan-binding molecules.
KW - Brain
KW - Glycosaminoglycan
KW - Growth factors
KW - Hydrogel
KW - Neural stem cell
KW - Polyelectrolyte complex nanoparticle
UR - http://www.scopus.com/inward/record.url?scp=85048027053&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048027053&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2018.05.009
DO - 10.1016/j.biomaterials.2018.05.009
M3 - Article
C2 - 29763775
AN - SCOPUS:85048027053
VL - 174
SP - 17
EP - 30
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
ER -