Glutamine modulates sepsis-induced changes to intestinal intraepithelial γδT lymphocyte expression in mice

Wan Yun Lee, Ya Mei Hu, Tsui Ling Ko, Sung Ling Yeh, Chiu Li Yeh

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study investigated the effect of glutamine (GLN) on intestinal intraepithelial lymphocyte (IEL) γδT-cell cytokines and immune regulatory factor gene expressions in a mouse model of polymicrobial sepsis. Mice were randomly assigned to a normal group, a sepsis with saline (SS) group, or a sepsis with GLN (SG) group. All mice were fed a chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The SS group was injected with saline, and the SG group was given 0.75 g GLN/kg body weight once via a tail vein 1 h after CLP. Septic mice were killed 12 h after CLP, and IEL γδT cells of the animals were isolated for further analysis. Results showed that compared with normal mice, sepsis resulted in lower IEL γδT-cell percentage and higher messenger RNA expressions of interferon γ, tumor necrosis factor α, interleukin 4 (IL-4), IL-13, IL-17, retinoid acid receptor-related orphan receptor γt, and complement 5a receptor by IEL γδT cells. These immunomodulatory mediator genes exhibited decreases, whereas IL-7 receptor expression increased in IEL γδT cells in septic mice with GLN administration. Annexin V/7-amino-actinomycin D stain revealed significantly lower rates of apoptosis, and IEL γδT-cell percentage was higher in the SG group. The histological findings also showed that damage to intestinal epithelial cells was less severe in the SG group. These results indicated that a single dose of GLN administered as treatment after the initiation of sepsis prevented apoptosis of IEL γδT cells and downregulated γδT cell-expressed inflammatory mediators that may consequently ameliorate the severity of sepsis-induced intestinal epithelial injury.

Original languageEnglish
Pages (from-to)288-293
Number of pages6
JournalShock
Volume38
Issue number3
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

Fingerprint

Glutamine
Sepsis
T-Lymphocytes
Lymphocytes
Punctures
Ligation
Interleukin-7 Receptors
Anaphylatoxin C5a Receptor
Apoptosis
Interleukin-13
Interleukin-17
Annexin A5
Retinoids
Immunologic Factors
Regulator Genes
Interleukin-4
Interferons
Tail
Veins
Coloring Agents

Keywords

  • apoptosis
  • complement 5a receptor
  • interleukin 13
  • interleukin 17
  • retinoid acid receptor Yrelated orphan receptor γt

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Glutamine modulates sepsis-induced changes to intestinal intraepithelial γδT lymphocyte expression in mice. / Lee, Wan Yun; Hu, Ya Mei; Ko, Tsui Ling; Yeh, Sung Ling; Yeh, Chiu Li.

In: Shock, Vol. 38, No. 3, 08.2012, p. 288-293.

Research output: Contribution to journalArticle

Lee, Wan Yun ; Hu, Ya Mei ; Ko, Tsui Ling ; Yeh, Sung Ling ; Yeh, Chiu Li. / Glutamine modulates sepsis-induced changes to intestinal intraepithelial γδT lymphocyte expression in mice. In: Shock. 2012 ; Vol. 38, No. 3. pp. 288-293.
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