補充麩醯胺對敗血症小鼠肝臟發炎反應及氧化壓力之影響

Translated title of the contribution: Glutamine Modulates Liver Inflammation And Oxidative Stress in Septic Mice

陳姿秀, 王貝文, 黃亭潔, 吳尚鴻, 葉秋莉

Research output: Contribution to journalArticle

Abstract

This study investigated the effect of intravenous glutamine (GLN) supplementation on the liver's inflammatory response and oxidative stress in a mouse model of polymicrobial sepsis. Mice were randomly assigned to a normal control (NC) group and two sepsis groups. Sepsis was induced by cecal ligation and puncture (CLP). One hour after CLP, septic mice were given saline (SS) or 0.75 g GLN/kg of body weight (SG) once via a tail vein. Septic mice were sacrificed at 24 or 48 h after CLP, and the livers were harvested for further analysis. Results showed that sepsis resulted in higher myeloperoxidase (MPO) activity and malondialdehyde (MDA) production. Interleukin (IU-1, IL-6, and tumor necrosis factor (TNF)-α levels in the liver were upregulated, whereas IL-10 and transforming growth factor (TGF)-β had decreased at 24 and/or 48 h after CLP. Compared to the SS group, the SG group had lower concentrations of IL-1, IL-6, and TNF-α and higher IL-10 and TGF-β levels. Also, MPO activity and MDA concentrations were reduced in liver tissues. These results suggest that sepsis results in inflammation of liver tissues. A single dose of intravenous GLN administration after CLP reduced oxidative stress and attenuated the inflammatory response in livers of mice with polymicrobial sepsis.
Original languageTraditional Chinese
Pages (from-to)113-121
Number of pages9
JournalJournal of the Chinese Nutrition Society
Volume40
Issue number3
Publication statusPublished - Sep 1 2015
Externally publishedYes

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Glutamine
Sepsis
Punctures
Oxidative Stress
Ligation
Inflammation
Liver
Transforming Growth Factors
Malondialdehyde
Interleukin-1
Interleukin-10
Peroxidase
Interleukin-6
Tumor Necrosis Factor-alpha
Intravenous Administration
Tail
Veins
Body Weight
Control Groups

Keywords

  • sepsis
  • glutamine
  • liver
  • proinflammatory cytokine
  • oxidative stress

Cite this

補充麩醯胺對敗血症小鼠肝臟發炎反應及氧化壓力之影響. / 陳姿秀; 王貝文; 黃亭潔; 吳尚鴻; 葉秋莉.

In: Journal of the Chinese Nutrition Society, Vol. 40, No. 3, 01.09.2015, p. 113-121.

Research output: Contribution to journalArticle

陳姿秀 ; 王貝文 ; 黃亭潔 ; 吳尚鴻 ; 葉秋莉. / 補充麩醯胺對敗血症小鼠肝臟發炎反應及氧化壓力之影響. In: Journal of the Chinese Nutrition Society. 2015 ; Vol. 40, No. 3. pp. 113-121.
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abstract = "實驗觀察給與敗血症小鼠麩醯胺補充後對其肝臟發炎狀態及氧化壓力的影響。C57BL/6小鼠分為正常控制組(normal control, NC)及敗血症(sepsis)組。敗血症利用盲腸結紮並穿刺手術(cecal ligation and puncture,CLP)引致。CLP後1小時利用尾靜脈注射生理食鹽水(sepsissaline, SS)或麩醯胺溶液(sepsis glutamine , SG)。麩醯胺給與劑量為0.75 g GLN/kg體重。敗血症後24及48小時將老鼠犧牲,取肝臟分析。結果顯示,與控制組相較,肝臟中interleukin (IL) -1, IL-6及tumor necrosis factor (TNF) -α的濃度在敗血症發生後24或48小時顯著增加,而IL-10及transforming growth factor (TGF) -β濃度則降低。CLP後肝臟骨髓過氧化酵素(myeloperoxidase, MPO)活性及脂質過氧化物丙二醛(malondialdehyde, MDA)皆比控制組高。與SS組相較,SG組在敗血症發生後24小時肝臟中IL-6濃度明顯較低, 並在敗血症後的小時明顯減少IL-1、IL-6及TNF-α的表現。CLP後48小時SG組老鼠肝臟中IL-10及TGF-β濃度高於SS組。敗血症發生後24及48小時,SG組老鼠肝臟MPO活性及MDA濃度比SS組低。根據結果顯示,敗血症發生後給予老鼠尾靜脈注射GLN,可以減少肝臟發炎反應及氧化壓力。",
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T1 - 補充麩醯胺對敗血症小鼠肝臟發炎反應及氧化壓力之影響

AU - 陳姿秀, null

AU - 王貝文, null

AU - 黃亭潔, null

AU - 吳尚鴻, null

AU - 葉秋莉, null

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N2 - 實驗觀察給與敗血症小鼠麩醯胺補充後對其肝臟發炎狀態及氧化壓力的影響。C57BL/6小鼠分為正常控制組(normal control, NC)及敗血症(sepsis)組。敗血症利用盲腸結紮並穿刺手術(cecal ligation and puncture,CLP)引致。CLP後1小時利用尾靜脈注射生理食鹽水(sepsissaline, SS)或麩醯胺溶液(sepsis glutamine , SG)。麩醯胺給與劑量為0.75 g GLN/kg體重。敗血症後24及48小時將老鼠犧牲,取肝臟分析。結果顯示,與控制組相較,肝臟中interleukin (IL) -1, IL-6及tumor necrosis factor (TNF) -α的濃度在敗血症發生後24或48小時顯著增加,而IL-10及transforming growth factor (TGF) -β濃度則降低。CLP後肝臟骨髓過氧化酵素(myeloperoxidase, MPO)活性及脂質過氧化物丙二醛(malondialdehyde, MDA)皆比控制組高。與SS組相較,SG組在敗血症發生後24小時肝臟中IL-6濃度明顯較低, 並在敗血症後的小時明顯減少IL-1、IL-6及TNF-α的表現。CLP後48小時SG組老鼠肝臟中IL-10及TGF-β濃度高於SS組。敗血症發生後24及48小時,SG組老鼠肝臟MPO活性及MDA濃度比SS組低。根據結果顯示,敗血症發生後給予老鼠尾靜脈注射GLN,可以減少肝臟發炎反應及氧化壓力。

AB - 實驗觀察給與敗血症小鼠麩醯胺補充後對其肝臟發炎狀態及氧化壓力的影響。C57BL/6小鼠分為正常控制組(normal control, NC)及敗血症(sepsis)組。敗血症利用盲腸結紮並穿刺手術(cecal ligation and puncture,CLP)引致。CLP後1小時利用尾靜脈注射生理食鹽水(sepsissaline, SS)或麩醯胺溶液(sepsis glutamine , SG)。麩醯胺給與劑量為0.75 g GLN/kg體重。敗血症後24及48小時將老鼠犧牲,取肝臟分析。結果顯示,與控制組相較,肝臟中interleukin (IL) -1, IL-6及tumor necrosis factor (TNF) -α的濃度在敗血症發生後24或48小時顯著增加,而IL-10及transforming growth factor (TGF) -β濃度則降低。CLP後肝臟骨髓過氧化酵素(myeloperoxidase, MPO)活性及脂質過氧化物丙二醛(malondialdehyde, MDA)皆比控制組高。與SS組相較,SG組在敗血症發生後24小時肝臟中IL-6濃度明顯較低, 並在敗血症後的小時明顯減少IL-1、IL-6及TNF-α的表現。CLP後48小時SG組老鼠肝臟中IL-10及TGF-β濃度高於SS組。敗血症發生後24及48小時,SG組老鼠肝臟MPO活性及MDA濃度比SS組低。根據結果顯示,敗血症發生後給予老鼠尾靜脈注射GLN,可以減少肝臟發炎反應及氧化壓力。

KW - 敗血症

KW - 麩醯胺

KW - 肝臟

KW - 促發炎反應細胞激素

KW - 氧化壓力

KW - sepsis

KW - glutamine

KW - liver

KW - proinflammatory cytokine

KW - oxidative stress

M3 - 文章

VL - 40

SP - 113

EP - 121

JO - Journal of the Chinese Nutrition Society

JF - Journal of the Chinese Nutrition Society

SN - 1011-6958

IS - 3

ER -