Glutamine modulates lipopolysaccharide-induced activation of NF- kB via the AKt/mTOR pathway in lung epithelial cells

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30 Citations (Scopus)

Abstract

Lung epithelial cells are important barriers in the respiratory system that provoke inflammatory responses through nuclear factor (NF)-kB activation to prevent pathogens from invading the body. Lipopolysaccharide (LPS) is a common pathogen-associated stimulus that activates IkB kinase (IKK) to regulate NF- kB-mediated inflammation through modulating nuclear translocation and phosphorylation of NF- kB. Previously, it was shown that Akt and the mammalian target of rapamycin (mTOR) are involved in the phosphorylation of IKK to activate NF- kB. Herein, we demonstrate that glutamine (GLN) modulated LPS-induced activation of NF- kB through the Akt/mTOR/IKK pathway in BEAS-2B cells. BEAS-2B cells in submerged culture were placed in medium containing different concentrations of GLN (0, 0.5, 1, and 2.5 mM) with 1 kg/ml LPS. Results showed that GLN deprivation induced phosphorylation of Akt/mTOR/IKK signaling, increased levels of NF- kB nuclear translocation and phosphorylated NF- kB, and upregulated NF- kB-dependent transcriptional activity, which was suppressed by GLN administration. Expressions of NF- kB-targeted genes were also reduced by supplemental GLN. GLN administration improved cell viability, whereas 0.5 mM GLN had a greater extent of inhibition on the Akt/mTOR/IKK/NF- kB signaling cascade. The inhibitory effects of GLN on NF- kB activation were also observed in cells cultured under air-liquid interface condition. These results indicate that GLN deprivation increased LPS-induced NF- kB activation and transcriptional activity, which was reversed by GLN administration. The findings provide potential mechanisms of GLN's modulation of LPSinduced NF- kB activation in lung epithelial cells and imply that maintaining a physiological concentration of GLN is essential in preventing LPS-induced lung inflammation.

Original languageEnglish
Pages (from-to)L174-L183
Number of pages10
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume302
Issue number1
DOIs
Publication statusPublished - Jan 2012

Keywords

  • BEAS-2B
  • Mammalian target of rapamycin
  • Nuclear factor- B

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

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