Glutamine modulates acute dextran sulphate sodium-induced changes in small-intestinal intraepithelial γδ-T-lymphocyte expression in mice

Man Hui Pai, Jun Jen Liu, Sung Ling Yeh, Wei Jao Chen, Chiu Li Yeh

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5 Citations (Scopus)

Abstract

The present study investigated the effect of glutamine (Gln) on dextran sulphate sodium (DSS)-induced changes in the expression of small-intestinal intraepithelial lymphocyte (IEL) γδ-T cells in mice. Mice were randomly assigned to a normal control (NC) group and two DSS-treated groups. The NC group and one of the DSS-treated groups (DSS-C) were fed a common semi-purified diet, while the other DSS-treated group (DSS-G) was fed an identical diet, except that part of casein was replaced by Gln, which provided 25% of total amino acid nitrogen. After being fed the diets for 10d, mice in the NC group were given distilled water, while the DSS-treated groups were given distilled water containing 2.5% DSS for 5d. At the end of the experiment, the mice were killed. The small-intestinal IEL γδ-T-cell subset was isolated for further analysis. The results indicated that DSS treatment resulted in a lower percentage of small-intestinal IEL γδ-T cells and higher mRNA expressions of interferon-γ, TNF-α, IL-17, complement 5a receptor and keratinocyte growth factor in IEL γδ-T cells. Gln administration increased the proportion of small-intestinal IEL γδ-T cells, and the expression levels of immunomodulatory mediator genes in IEL γδ-T cells were lower in the DSS-treated mice. The histological findings indicated that the immunoreactive intensity of the tight junction protein ZO-1 in the small-intestinal mucosa was higher in the DSS-G group than in the DSS-C group. These results indicate that pretreatment with Gln increases the proportion of small-intestinal IEL γδ-T cells and down-regulates γδ-T-cell-expressed inflammatory mediators, which may consequently ameliorate the severity of DSS-induced small-intestinal epithelial injury.

Original languageEnglish
Pages (from-to)1032-1039
Number of pages8
JournalBritish Journal of Nutrition
Volume111
Issue number6
DOIs
Publication statusPublished - Mar 28 2014

Fingerprint

Dextran Sulfate
Glutamine
T-Lymphocytes
Lymphocytes
Diet
Control Groups
Anaphylatoxin C5a Receptor
Zonula Occludens-1 Protein
Fibroblast Growth Factor 7
Interleukin-17
Water
T-Lymphocyte Subsets
Intestinal Mucosa
Caseins
Interferons
Nitrogen
Down-Regulation

Keywords

  • Dextran sulphate sodium
  • Glutamine
  • Inflammatory mediators
  • Small-intestinal intraepithelial lymphocytes
  • ZO-1 tight junction protein
  • γδ-T cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

@article{72c0cde44e0343198b405a5b421e88b1,
title = "Glutamine modulates acute dextran sulphate sodium-induced changes in small-intestinal intraepithelial γδ-T-lymphocyte expression in mice",
abstract = "The present study investigated the effect of glutamine (Gln) on dextran sulphate sodium (DSS)-induced changes in the expression of small-intestinal intraepithelial lymphocyte (IEL) γδ-T cells in mice. Mice were randomly assigned to a normal control (NC) group and two DSS-treated groups. The NC group and one of the DSS-treated groups (DSS-C) were fed a common semi-purified diet, while the other DSS-treated group (DSS-G) was fed an identical diet, except that part of casein was replaced by Gln, which provided 25{\%} of total amino acid nitrogen. After being fed the diets for 10d, mice in the NC group were given distilled water, while the DSS-treated groups were given distilled water containing 2.5{\%} DSS for 5d. At the end of the experiment, the mice were killed. The small-intestinal IEL γδ-T-cell subset was isolated for further analysis. The results indicated that DSS treatment resulted in a lower percentage of small-intestinal IEL γδ-T cells and higher mRNA expressions of interferon-γ, TNF-α, IL-17, complement 5a receptor and keratinocyte growth factor in IEL γδ-T cells. Gln administration increased the proportion of small-intestinal IEL γδ-T cells, and the expression levels of immunomodulatory mediator genes in IEL γδ-T cells were lower in the DSS-treated mice. The histological findings indicated that the immunoreactive intensity of the tight junction protein ZO-1 in the small-intestinal mucosa was higher in the DSS-G group than in the DSS-C group. These results indicate that pretreatment with Gln increases the proportion of small-intestinal IEL γδ-T cells and down-regulates γδ-T-cell-expressed inflammatory mediators, which may consequently ameliorate the severity of DSS-induced small-intestinal epithelial injury.",
keywords = "Dextran sulphate sodium, Glutamine, Inflammatory mediators, Small-intestinal intraepithelial lymphocytes, ZO-1 tight junction protein, γδ-T cells, Dextran sulphate sodium, Glutamine, Inflammatory mediators, Small-intestinal intraepithelial lymphocytes, ZO-1 tight junction protein, γδ-T cells",
author = "Pai, {Man Hui} and Liu, {Jun Jen} and Yeh, {Sung Ling} and Chen, {Wei Jao} and Yeh, {Chiu Li}",
year = "2014",
month = "3",
day = "28",
doi = "10.1017/S0007114513003425",
language = "English",
volume = "111",
pages = "1032--1039",
journal = "British Journal of Nutrition",
issn = "0007-1145",
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TY - JOUR

T1 - Glutamine modulates acute dextran sulphate sodium-induced changes in small-intestinal intraepithelial γδ-T-lymphocyte expression in mice

AU - Pai, Man Hui

AU - Liu, Jun Jen

AU - Yeh, Sung Ling

AU - Chen, Wei Jao

AU - Yeh, Chiu Li

PY - 2014/3/28

Y1 - 2014/3/28

N2 - The present study investigated the effect of glutamine (Gln) on dextran sulphate sodium (DSS)-induced changes in the expression of small-intestinal intraepithelial lymphocyte (IEL) γδ-T cells in mice. Mice were randomly assigned to a normal control (NC) group and two DSS-treated groups. The NC group and one of the DSS-treated groups (DSS-C) were fed a common semi-purified diet, while the other DSS-treated group (DSS-G) was fed an identical diet, except that part of casein was replaced by Gln, which provided 25% of total amino acid nitrogen. After being fed the diets for 10d, mice in the NC group were given distilled water, while the DSS-treated groups were given distilled water containing 2.5% DSS for 5d. At the end of the experiment, the mice were killed. The small-intestinal IEL γδ-T-cell subset was isolated for further analysis. The results indicated that DSS treatment resulted in a lower percentage of small-intestinal IEL γδ-T cells and higher mRNA expressions of interferon-γ, TNF-α, IL-17, complement 5a receptor and keratinocyte growth factor in IEL γδ-T cells. Gln administration increased the proportion of small-intestinal IEL γδ-T cells, and the expression levels of immunomodulatory mediator genes in IEL γδ-T cells were lower in the DSS-treated mice. The histological findings indicated that the immunoreactive intensity of the tight junction protein ZO-1 in the small-intestinal mucosa was higher in the DSS-G group than in the DSS-C group. These results indicate that pretreatment with Gln increases the proportion of small-intestinal IEL γδ-T cells and down-regulates γδ-T-cell-expressed inflammatory mediators, which may consequently ameliorate the severity of DSS-induced small-intestinal epithelial injury.

AB - The present study investigated the effect of glutamine (Gln) on dextran sulphate sodium (DSS)-induced changes in the expression of small-intestinal intraepithelial lymphocyte (IEL) γδ-T cells in mice. Mice were randomly assigned to a normal control (NC) group and two DSS-treated groups. The NC group and one of the DSS-treated groups (DSS-C) were fed a common semi-purified diet, while the other DSS-treated group (DSS-G) was fed an identical diet, except that part of casein was replaced by Gln, which provided 25% of total amino acid nitrogen. After being fed the diets for 10d, mice in the NC group were given distilled water, while the DSS-treated groups were given distilled water containing 2.5% DSS for 5d. At the end of the experiment, the mice were killed. The small-intestinal IEL γδ-T-cell subset was isolated for further analysis. The results indicated that DSS treatment resulted in a lower percentage of small-intestinal IEL γδ-T cells and higher mRNA expressions of interferon-γ, TNF-α, IL-17, complement 5a receptor and keratinocyte growth factor in IEL γδ-T cells. Gln administration increased the proportion of small-intestinal IEL γδ-T cells, and the expression levels of immunomodulatory mediator genes in IEL γδ-T cells were lower in the DSS-treated mice. The histological findings indicated that the immunoreactive intensity of the tight junction protein ZO-1 in the small-intestinal mucosa was higher in the DSS-G group than in the DSS-C group. These results indicate that pretreatment with Gln increases the proportion of small-intestinal IEL γδ-T cells and down-regulates γδ-T-cell-expressed inflammatory mediators, which may consequently ameliorate the severity of DSS-induced small-intestinal epithelial injury.

KW - Dextran sulphate sodium

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KW - Small-intestinal intraepithelial lymphocytes

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KW - Dextran sulphate sodium

KW - Glutamine

KW - Inflammatory mediators

KW - Small-intestinal intraepithelial lymphocytes

KW - ZO-1 tight junction protein

KW - γδ-T cells

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U2 - 10.1017/S0007114513003425

DO - 10.1017/S0007114513003425

M3 - Article

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JO - British Journal of Nutrition

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SN - 0007-1145

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