Glutamine modulates acute dextran sulphate sodium-induced changes in small-intestinal intraepithelial γδ-T-lymphocyte expression in mice

Man Hui Pai, Jun Jen Liu, Sung Ling Yeh, Wei Jao Chen, Chiu Li Yeh

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5 Citations (Scopus)

Abstract

The present study investigated the effect of glutamine (Gln) on dextran sulphate sodium (DSS)-induced changes in the expression of small-intestinal intraepithelial lymphocyte (IEL) γδ-T cells in mice. Mice were randomly assigned to a normal control (NC) group and two DSS-treated groups. The NC group and one of the DSS-treated groups (DSS-C) were fed a common semi-purified diet, while the other DSS-treated group (DSS-G) was fed an identical diet, except that part of casein was replaced by Gln, which provided 25% of total amino acid nitrogen. After being fed the diets for 10d, mice in the NC group were given distilled water, while the DSS-treated groups were given distilled water containing 2.5% DSS for 5d. At the end of the experiment, the mice were killed. The small-intestinal IEL γδ-T-cell subset was isolated for further analysis. The results indicated that DSS treatment resulted in a lower percentage of small-intestinal IEL γδ-T cells and higher mRNA expressions of interferon-γ, TNF-α, IL-17, complement 5a receptor and keratinocyte growth factor in IEL γδ-T cells. Gln administration increased the proportion of small-intestinal IEL γδ-T cells, and the expression levels of immunomodulatory mediator genes in IEL γδ-T cells were lower in the DSS-treated mice. The histological findings indicated that the immunoreactive intensity of the tight junction protein ZO-1 in the small-intestinal mucosa was higher in the DSS-G group than in the DSS-C group. These results indicate that pretreatment with Gln increases the proportion of small-intestinal IEL γδ-T cells and down-regulates γδ-T-cell-expressed inflammatory mediators, which may consequently ameliorate the severity of DSS-induced small-intestinal epithelial injury.

Original languageEnglish
Pages (from-to)1032-1039
Number of pages8
JournalBritish Journal of Nutrition
Volume111
Issue number6
DOIs
Publication statusPublished - Mar 28 2014

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Keywords

  • Dextran sulphate sodium
  • Glutamine
  • Inflammatory mediators
  • Small-intestinal intraepithelial lymphocytes
  • ZO-1 tight junction protein
  • γδ-T cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

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